wt1a WT1 transcription factor a [Danio rerio (zebrafish)] - Gene

Summary

Official Symbol: wt1aprovided by ZNC
Official Full Name: WT1 transcription factor aprovided by ZNC
Primary source: ZFIN:ZDB-GENE-980526-558
See related: Ensembl:ENSDARG00000031420 AllianceGenome:ZFIN:ZDB-GENE-980526-558
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Danio rerio
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; Danionidae; Danioninae; Danio
Also known as: wt1; zwt1
Summary: Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Acts upstream of or within animal organ development. Predicted to be located in cytoplasm and nuclear speck. Is expressed in several structures, including cardiovascular system; mesoderm; renal system; reproductive system; and spleen. Human ortholog(s) of this gene implicated in several diseases, including Denys-Drash syndrome; Frasier syndrome; malignant mesothelioma; nephroblastoma; and nephrotic syndrome type 4. Orthologous to human WT1 (WT1 transcription factor). [provided by Alliance of Genome Resources, Apr 2022]
Orthologs: human mouse all
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Genomic context

Location:: chromosome: 25

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCz11 (GCF_000002035.6)	25	NC_007136.7 (15176481..15214335, complement)
105	previous assembly	GRCz10 (GCF_000002035.5)	25	NC_007136.6 (15081825..15117648, complement)

Chromosome 25 - NC_007136.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
Description: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
BioProject: PRJEB1986
Analysis date: Fri Dec 8 19:48:10 2017

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Wt1 transcription factor impairs cardiomyocyte specification and drives a phenotypic switch from myocardium to epicardium.
Title: Wt1 transcription factor impairs cardiomyocyte specification and drives a phenotypic switch from myocardium to epicardium.
Analysis of wt1a reporter line expression levels during proepicardium formation in the zebrafish.
Title: Analysis of wt1a reporter line expression levels during proepicardium formation in the zebrafish.
Study summarizes the different roles of WT1a gene in the embryonic zebrafish kidney, the adult kidney and the cardiac development as well. [review]
Title: Function and Regulation of the Wilms' Tumor Suppressor 1 (WT1) Gene in Fish.
Results indicate that Jagged-Notch signaling is required for segregation between wt1-expressing cells and differentiated steroidogenic tissue.
Title: Aberrant Global and Jagged-Mediated Notch Signaling Disrupts Segregation Between wt1-Expressing and Steroidogenic Tissues in Zebrafish.
In summary, our results show that WT1 is a crucial transcription factor for podocyte maturation and maintenance.
Title: Integration of Cistromic and Transcriptomic Analyses Identifies Nphs2, Mafb, and Magi2 as Wilms' Tumor 1 Target Genes in Podocyte Differentiation and Maintenance.
Knockdown of wt1a disrupted podocyte differentiation and prevented expression of osr1.
Title: osr1 is required for podocyte development downstream of wt1a.
embryos treated with morpholinos against wt1a, foxc1a, or the Notch transcriptional mediator rbpj develop fewer podocytes, as determined by wt1b, hey1 and nephrin expression, while embryos deficient in any two of these factors completely lack podocytes
Title: Wt1a, Foxc1a, and the Notch mediator Rbpj physically interact and regulate the formation of podocytes in zebrafish.
Results describe the first functional demonstration of defined regulatory elements controlling Wt1 expression in vivo.
Title: A highly conserved retinoic acid responsive element controls wt1a expression in the zebrafish pronephros.
While wt1a has a more fundamental and early role in pronephros development and is essential for the formation of glomerular structures, wt1b functions at later stages of nephrogenesis.
Title: The Wilms tumor genes wt1a and wt1b control different steps during formation of the zebrafish pronephros.
Both wt1a and wt1b are expressed in the intermediate mesoderm, with wt1b being restricted to a smaller area lying at the caudal end of the wt1a expression domain
Title: Identification and comparative expression analysis of a second wt1 gene in zebrafish.

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General gene information

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Markers

X85734 (e-PCR)
- UniSTS:205449

wt1 (e-PCR), detects polymorphism
- UniSTS:238224

X85735 (e-PCR)
- UniSTS:205465

Gene Ontology Provided by ZFIN

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within adrenal gland development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within adrenal gland development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in anatomical structure morphogenesis	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within glomerulus development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of apoptotic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of cell population proliferation	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within podocyte cell fate commitment	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within podocyte differentiation	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within podocyte differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within pronephric duct development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within pronephric glomerulus development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within pronephric glomerulus development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within pronephros development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within pronephros development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of blood-brain barrier permeability	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within visceral serous pericardium development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within visceral serous pericardium development	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in nuclear speck	IEA Inferred from Electronic Annotation more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: WT1 transcription factor a

Names: wilms tumor 1a

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_131046.2 → NP_571121.1 WT1 transcription factor a

See identical proteins and their annotated locations for NP_571121.1

Status: VALIDATED

Source sequence(s)

CR762411, CU928058

UniProtKB/TrEMBL

B3DGC5, Q9PUT7

Related

ENSDARP00000029174.8, ENSDART00000031499.9

Conserved Domains (4) summary

COG5048
Location:286 → 411

COG5048; FOG: Zn-finger [General function prediction only]

sd00017
Location:298 → 317

ZF_C2H2; C2H2 Zn finger [structural motif]

pfam02165
Location:1 → 291

WT1; Wilm's tumour protein

pfam13465
Location:309 → 336

zf-H2C2_2; Zinc-finger double domain