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MCM3 minichromosome maintenance complex component 3 [ Homo sapiens (human) ]

Gene ID: 4172, updated on 3-Nov-2024

Summary

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Official Symbol
MCM3provided by HGNC
Official Full Name
minichromosome maintenance complex component 3provided by HGNC
Primary source
HGNC:HGNC:6945
See related
Ensembl:ENSG00000112118 MIM:602693; AllianceGenome:HGNC:6945
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
HCC5; P1.h; RLFB; P1-MCM3
Summary
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2018]
Expression
Ubiquitous expression in lymph node (RPKM 33.0), appendix (RPKM 23.5) and 24 other tissues See more
Orthologs
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Genomic context

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See MCM3 in Genome Data Viewer
Location:
6p12.2
Exon count:
20
Annotation release Status Assembly Chr Location
RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 6 NC_000006.12 (52264015..52284742, complement)
RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 6 NC_060930.1 (52102764..52123487, complement)
RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 6 NC_000006.11 (52128813..52149540, complement)

Chromosome 6 - NC_000006.12Genomic Context describing neighboring genes Neighboring gene uncharacterized LOC124901328 Neighboring gene solute carrier family 25 member 20 pseudogene 1 Neighboring gene interleukin 17F Neighboring gene ReSE screen-validated silencer GRCh37_chr6:52129168-52129587 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 24670 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 24671 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 17279 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 24672 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 17280 Neighboring gene uncharacterized LOC124901329 Neighboring gene Sharpr-MPRA regulatory region 10632 Neighboring gene Sharpr-MPRA regulatory region 10484 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 24673 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 17281 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 17282 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 24674 Neighboring gene OCT4-NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr6:52190539-52191144 Neighboring gene OCT4-NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr6:52191145-52191751 Neighboring gene Neanderthal introgressed variant-containing enhancer experimental_92086 Neighboring gene Sharpr-MPRA regulatory region 5108 Neighboring gene H3K27ac hESC enhancer GRCh37_chr6:52217075-52217574 Neighboring gene ReSE screen-validated silencer GRCh37_chr6:52226353-52226538 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 17283 Neighboring gene progestin and adipoQ receptor family member 8 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 24676 Neighboring gene VISTA enhancer hs2064 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 24678

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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Related articles in PubMed

  1. MCM3 as a novel diagnostic marker in benign and malignant salivary gland tumors. Ashkavandi ZJ, et al. Asian Pac J Cancer Prev, 2013. PMID 23886132
  2. Cancer-associated expression of minichromosome maintenance 3 gene in several human cancers and its involvement in tumorigenesis. Ha SA, et al. Clin Cancer Res, 2004 Dec 15. PMID 15623617
  3. Minichromosome maintenance protein 3 elicits a cancer-restricted immune response in patients with brain malignancies and is a strong independent predictor of survival in patients with anaplastic astrocytoma. Söling A, et al. Clin Cancer Res, 2005 Jan 1. PMID 15671553
  4. Human cytomegalovirus IE86 protein binds to cellular Mcm3 protein but does not inhibit its binding to the Epstein-Barr virus oriP in U373MG-p220.2 cells. Song YJ, et al. Acta Virol, 2010. PMID 20545442
  5. MCM3 is a novel proliferation marker associated with longer survival for patients with tubo-ovarian high-grade serous carcinoma. Kang EY, et al. Virchows Arch, 2022 Apr. PMID 34782936, Free PMC Article

See all (328) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. MCM3 is a novel proliferation marker associated with longer survival for patients with tubo-ovarian high-grade serous carcinoma.
    Title: MCM3 is a novel proliferation marker associated with longer survival for patients with tubo-ovarian high-grade serous carcinoma.
  2. MCM complex members MCM3 and MCM7 are associated with a phenotypic spectrum from Meier-Gorlin syndrome to lipodystrophy and adrenal insufficiency.
    Title: MCM complex members MCM3 and MCM7 are associated with a phenotypic spectrum from Meier-Gorlin syndrome to lipodystrophy and adrenal insufficiency.
  3. Single-cell chromatin accessibility landscape of human umbilical cord blood in trisomy 18 syndrome.
    Title: Single-cell chromatin accessibility landscape of human umbilical cord blood in trisomy 18 syndrome.
  4. Bioinformatics Analysis Reveals MCM3 as an Important Prognostic Marker in Cervical Cancer.
    Title: Bioinformatics Analysis Reveals MCM3 as an Important Prognostic Marker in Cervical Cancer.
  5. The Immunohistochemical Expression of MCM-3, -5, and -7 Proteins in the Uterine Fibroids.
    Title: The Immunohistochemical Expression of MCM-3, -5, and -7 Proteins in the Uterine Fibroids.
  6. PLK1 promotes proliferation and suppresses apoptosis of renal cell carcinoma cells by phosphorylating MCM3.
    Title: PLK1 promotes proliferation and suppresses apoptosis of renal cell carcinoma cells by phosphorylating MCM3.
  7. MCM3 proliferative index is worthier over Ki-67 in the characterization of salivary gland tumors.
    Title: MCM3 proliferative index is worthier over Ki-67 in the characterization of salivary gland tumors.
  8. Elevated expression of minichromosome maintenance 3 indicates poor outcomes and promotes G1/S cell cycle progression, proliferation, migration and invasion in colorectal cancer.
    Title: Elevated expression of minichromosome maintenance 3 indicates poor outcomes and promotes G1/S cell cycle progression, proliferation, migration and invasion in colorectal cancer.
  9. Gene expression profiling revealed MCM3 to be a better marker than Ki67 in prognosis of invasive ductal breast carcinoma patients.
    Title: Gene expression profiling revealed MCM3 to be a better marker than Ki67 in prognosis of invasive ductal breast carcinoma patients.
  10. Results show that found DNA replication initiation protein MCM3 was upregulated in hepatocellular carcinoma (HCC) tissues and cells and its expression correlated with poor outcome and radioresistance. MCM3 promoted radioresistance through activating NF-kappaB pathway, strengthening the role of MCM subunits in the tumor progression. These Findings revealed an independent prognostic factor of MCM3 for HCC.
    Title: Minichromosome maintenance 3 promotes hepatocellular carcinoma radioresistance by activating the NF-κB pathway.
Submit: New GeneRIF Correction See all GeneRIFs (45)

Phenotypes

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Review eQTL and phenotype association data in this region using PheGenI

Variation

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See Variation Viewer (GRCh37.p13)

HIV-1 interactions

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Protein interactions

Protein Gene Interaction Pubs
Rev rev HIV-1 Rev co-localizes with minichromosome maintenance complex component 3 (MCM3) in the cytoplasmic and nucleoplasmic compartments of cells PubMed
Tat tat HIV-1 Tat upregulates the expression of minichromosome maintenance complex component 3 (MCM3) in Jurkat cells PubMed
tat Interaction of HIV-1 Tat with MCM3 in T-cells is identified by a proteomic strategy based on affinity chromatography coupled with mass spectrometry PubMed
Vpr vpr HIV-1 Vpr downregulates MCM3 in HeLa cells within 12 hours of exposure PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Clone Names

  • MGC1157

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables ATP hydrolysis activity IEA
Inferred from Electronic Annotation
more info
  
enables DNA binding TAS
Traceable Author Statement
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables single-stranded DNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
contributes_to single-stranded DNA helicase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
Process Evidence Code Pubs
involved_in DNA replication initiation TAS
Traceable Author Statement
more info
 PubMed 
involved_in DNA strand elongation involved in DNA replication IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in DNA unwinding involved in DNA replication IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in double-strand break repair via break-induced replication IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in mitotic DNA replication initiation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in regulation of DNA-templated DNA replication initiation NAS
Non-traceable Author Statement
more info
 PubMed 
Component Evidence Code Pubs
part_of CMG complex IPI
Inferred from Physical Interaction
more info
 PubMed 
part_of CMG complex ISS
Inferred from Sequence or Structural Similarity
more info
  
part_of MCM complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of MCM complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of MCM complex IPI
Inferred from Physical Interaction
more info
 PubMed 
part_of alpha DNA polymerase:primase complex TAS
Traceable Author Statement
more info
 PubMed 
located_in centrosome IDA
Inferred from Direct Assay
more info
  
located_in chromosome, telomeric region HDA PubMed 
located_in membrane HDA PubMed 
NOT located_in nucleolus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleoplasm IDA
Inferred from Direct Assay
more info
  
located_in nucleoplasm TAS
Traceable Author Statement
more info
  
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in perinuclear region of cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
DNA replication licensing factor MCM3
Names
DNA polymerase alpha holoenzyme-associated protein P1
DNA replication factor MCM3
MCM3 minichromosome maintenance deficient 3
RLF subunit beta
cervical cancer proto-oncogene 5
hRlf beta subunit
minichromosome maintenance deficient 3
p102
replication licensing factor, beta subunit
NP_001257401.2
NP_001353298.1
NP_001353299.1
NP_001353300.1
NP_001353301.1
NP_001353302.1
NP_001353303.1
NP_001353304.1
NP_002379.4

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001270472.3 → NP_001257401.2  DNA replication licensing factor MCM3 isoform 2

    Status: REVIEWED

    Source sequence(s)
    AL034343
    Consensus CDS
    CCDS75468.2
    UniProtKB/TrEMBL
    B4DUQ9, J3KQ69
    Related
    ENSP00000229854.6, ENST00000229854.12
    Conserved Domains (1) summary
    smart00350
    Location:63 → 608
    MCM; minichromosome maintenance proteins

  2. NM_001366369.2 → NP_001353298.1  DNA replication licensing factor MCM3 isoform 3

    Status: REVIEWED

    Source sequence(s)
    AL034343
    UniProtKB/TrEMBL
    A0A0S2Z492, Q53HJ4
    Conserved Domains (2) summary
    smart00350
    Location:109 → 654
    MCM; minichromosome maintenance proteins
    pfam14551
    Location:19 → 104
    MCM_N; MCM N-terminal domain

  3. NM_001366370.2 → NP_001353299.1  DNA replication licensing factor MCM3 isoform 4

    Status: REVIEWED

    Source sequence(s)
    AL034343
    UniProtKB/TrEMBL
    Q53HJ4
    Conserved Domains (2) summary
    smart00350
    Location:109 → 671
    MCM; minichromosome maintenance proteins
    pfam14551
    Location:19 → 104
    MCM_N; MCM N-terminal domain

  4. NM_001366371.2 → NP_001353300.1  DNA replication licensing factor MCM3 isoform 5

    Status: REVIEWED

    Source sequence(s)
    AL034343
    UniProtKB/TrEMBL
    Q53HJ4
    Conserved Domains (2) summary
    smart00350
    Location:109 → 637
    MCM; minichromosome maintenance proteins
    pfam14551
    Location:19 → 104
    MCM_N; MCM N-terminal domain

  5. NM_001366372.2 → NP_001353301.1  DNA replication licensing factor MCM3 isoform 6

    Status: REVIEWED

    Source sequence(s)
    AL034343
    UniProtKB/TrEMBL
    Q53HJ4
    Conserved Domains (2) summary
    smart00350
    Location:109 → 637
    MCM; minichromosome maintenance proteins
    pfam14551
    Location:19 → 104
    MCM_N; MCM N-terminal domain

  6. NM_001366373.2 → NP_001353302.1  DNA replication licensing factor MCM3 isoform 7

    Status: REVIEWED

    Source sequence(s)
    AL034343
    UniProtKB/TrEMBL
    B4DS46
    Conserved Domains (1) summary
    cl25443
    Location:6 → 481
    MCM_OB; MCM OB domain

  7. NM_001366374.2 → NP_001353303.1  DNA replication licensing factor MCM3 isoform 8

    Status: REVIEWED

    Source sequence(s)
    AL034343
    UniProtKB/TrEMBL
    B4DS46
    Conserved Domains (1) summary
    cl25443
    Location:1 → 451
    MCM_OB; MCM OB domain

  8. NM_001366375.2 → NP_001353304.1  DNA replication licensing factor MCM3 isoform 9

    Status: REVIEWED

    Source sequence(s)
    AL034343
    Conserved Domains (2) summary
    COG1241
    Location:17 → 165
    Mcm2; DNA replicative helicase MCM subunit Mcm2, Cdc46/Mcm family [Replication, recombination and repair]
    cl25443
    Location:177 → 373
    MCM_OB; MCM OB domain

  9. NM_002388.6 → NP_002379.4  DNA replication licensing factor MCM3 isoform 1

    Status: REVIEWED

    Source sequence(s)
    AL034343
    Consensus CDS
    CCDS4940.3
    UniProtKB/Swiss-Prot
    B4DWW4, P25205, Q92660, Q9BTR3, Q9NUE7
    UniProtKB/TrEMBL
    A0A0S2Z4T1, Q53HJ4
    Related
    ENSP00000472940.2, ENST00000596288.7
    Conserved Domains (2) summary
    smart00350
    Location:109 → 654
    MCM; minichromosome maintenance proteins
    pfam14551
    Location:16 → 102
    MCM_N; MCM N-terminal domain

RNA

  1. NR_158963.2 RNA Sequence

    Status: REVIEWED

    Source sequence(s)
    AL034343

  2. NR_158964.2 RNA Sequence

    Status: REVIEWED

    Source sequence(s)
    AL034343

  3. NR_158965.2 RNA Sequence

    Status: REVIEWED

    Source sequence(s)
    AL034343

  4. NR_158966.2 RNA Sequence

    Status: REVIEWED

    Source sequence(s)
    AL034343

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000006.12 Reference GRCh38.p14 Primary Assembly

    Range
    52264015..52284742 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060930.1 Alternate T2T-CHM13v2.0

    Range
    52102764..52123487 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P25205.3 GenPept UniProtKB/Swiss-Prot:P25205

Gene LinkOut

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