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RAD18 RAD18 E3 ubiquitin protein ligase [ Homo sapiens (human) ]

Gene ID: 56852, updated on 3-Nov-2024

Summary

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Official Symbol
RAD18provided by HGNC
Official Full Name
RAD18 E3 ubiquitin protein ligaseprovided by HGNC
Primary source
HGNC:HGNC:18278
See related
Ensembl:ENSG00000070950 MIM:605256; AllianceGenome:HGNC:18278
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
RNF73
Summary
The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. [provided by RefSeq, Jul 2008]
Expression
Ubiquitous expression in duodenum (RPKM 3.3), lymph node (RPKM 2.7) and 25 other tissues See more
Orthologs
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Genomic context

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See RAD18 in Genome Data Viewer
Location:
3p25.3
Exon count:
13
Annotation release Status Assembly Chr Location
RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 3 NC_000003.12 (8877075..8963472, complement)
RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 3 NC_060927.1 (8868283..8960543, complement)
RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 3 NC_000003.11 (8918759..9005156, complement)

Chromosome 3 - NC_000003.12Genomic Context describing neighboring genes Neighboring gene uncharacterized LOC107986061 Neighboring gene uncharacterized LOC107984112 Neighboring gene P300/CBP strongly-dependent group 1 enhancer GRCh37_chr3:8911034-8912233 Neighboring gene small nucleolar RNA SNORA43 Neighboring gene Sharpr-MPRA regulatory region 4351 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 19395 Neighboring gene Sharpr-MPRA regulatory region 3594 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr3:9029229-9029729 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr3:9028728-9029228 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr3:9038453-9038952 Neighboring gene SLIT-ROBO Rho GTPase activating protein 3 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr3:9119367-9119867 Neighboring gene H3K27ac hESC enhancer GRCh37_chr3:9146925-9147681 Neighboring gene uncharacterized LOC124909343 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 19396 Neighboring gene H3K27ac hESC enhancer GRCh37_chr3:9221274-9221774 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 14030 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 14031 Neighboring gene SRGAP3 antisense RNA 2

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Transcription Factor E2F7 Hampers the Killing Effect of NK Cells against Colorectal Cancer Cells via Activating RAD18 Transcription.
    Title: Transcription Factor E2F7 Hampers the Killing Effect of NK Cells against Colorectal Cancer Cells via Activating RAD18 Transcription.
  2. Implication of E3 ligase RAD18 in UV-induced mutagenesis in human induced pluripotent stem cells and neuronal progenitor cells.
    Title: Implication of E3 ligase RAD18 in UV-induced mutagenesis in human induced pluripotent stem cells and neuronal progenitor cells.
  3. Analysis of the Mechanism of RAD18 in Glioma.
    Title: Analysis of the Mechanism of RAD18 in Glioma.
  4. RAD18 opposes transcription-associated genome instability through FANCD2 recruitment.
    Title: RAD18 opposes transcription-associated genome instability through FANCD2 recruitment.
  5. RAD18 confers radioresistance of esophagus squamous cell carcinoma through regulating p-DNA-PKcs.
    Title: RAD18 confers radioresistance of esophagus squamous cell carcinoma through regulating p-DNA-PKcs.
  6. Genome-wide CRISPR screen identified Rad18 as a determinant of doxorubicin sensitivity in osteosarcoma.
    Title: Genome-wide CRISPR screen identified Rad18 as a determinant of doxorubicin sensitivity in osteosarcoma.
  7. UBE2B promotes ovarian cancer growth via promoting RAD18 mediated ZMYM2 monoubiquitination and stabilization.
    Title: UBE2B promotes ovarian cancer growth via promoting RAD18 mediated ZMYM2 monoubiquitination and stabilization.
  8. REV1 promotes lung tumorigenesis by activating the Rad18/SERTAD2 axis.
    Title: REV1 promotes lung tumorigenesis by activating the Rad18/SERTAD2 axis.
  9. RAD18 mediates DNA double-strand break-induced ubiquitination of chromatin protein.
    Title: RAD18 mediates DNA double-strand break-induced ubiquitination of chromatin protein.
  10. Translesion Synthesis or Repair by Specialized DNA Polymerases Limits Excessive Genomic Instability upon Replication Stress.
    Title: Translesion Synthesis or Repair by Specialized DNA Polymerases Limits Excessive Genomic Instability upon Replication Stress.
Submit: New GeneRIF Correction See all GeneRIFs (83)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See Variation Viewer (GRCh37.p13)

HIV-1 interactions

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Protein interactions

Protein Gene Interaction Pubs
integrase gag-pol HIV-1 Integrase interacts with (co-immunoprecipitates) and co-localizes with hRad18 suggesting a role for hRad18 in the DNA repair events associated with the HIV-1 integration process PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables Y-form DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables damaged DNA binding NAS
Non-traceable Author Statement
more info
 PubMed 
enables identical protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables polyubiquitin modification-dependent protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein-containing complex binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables single-stranded DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables ubiquitin protein ligase activity IEA
Inferred from Electronic Annotation
more info
  
enables ubiquitin protein ligase binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within DNA damage response IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in DNA damage response IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in DNA repair NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in positive regulation of chromosome segregation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in postreplication repair IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within protein autoubiquitination IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in protein monoubiquitination IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
part_of Rad6-Rad18 complex IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in centrosome IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in nuclear body IDA
Inferred from Direct Assay
more info
  
located_in nuclear inclusion body IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleoplasm IDA
Inferred from Direct Assay
more info
  
located_in nucleoplasm TAS
Traceable Author Statement
more info
  
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus NAS
Non-traceable Author Statement
more info
 PubMed 
located_in replication fork IDA
Inferred from Direct Assay
more info
 PubMed 
located_in site of double-strand break IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
E3 ubiquitin-protein ligase RAD18
Names
RAD18 homolog
RAD18, S. cerevisiae, homolog
RING finger protein 73
RING-type E3 ubiquitin transferase RAD18
hHR18
hRAD18
postreplication repair protein RAD18
postreplication repair protein hRAD18p
NP_064550.3
XP_016862362.1
XP_054203240.1
XP_054203241.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_020165.4 → NP_064550.3  E3 ubiquitin-protein ligase RAD18

    See identical proteins and their annotated locations for NP_064550.3

    Status: REVIEWED

    Source sequence(s)
    AC008151, AC034186, AK023075, DW433240
    Consensus CDS
    CCDS2571.1
    UniProtKB/Swiss-Prot
    Q58F55, Q9NRT6, Q9NS91
    UniProtKB/TrEMBL
    Q53H10
    Related
    ENSP00000264926.2, ENST00000264926.7
    Conserved Domains (4) summary
    smart00734
    Location:201 → 224
    ZnF_Rad18; Rad18-like CCHC zinc finger
    cd00162
    Location:25 → 63
    RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...
    TIGR00599
    Location:13 → 363
    rad18; DNA repair protein rad18
    pfam02037
    Location:248 → 282
    SAP; SAP domain

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000003.12 Reference GRCh38.p14 Primary Assembly

    Range
    8877075..8963472 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_017006873.2 → XP_016862362.1  E3 ubiquitin-protein ligase RAD18 isoform X1

    UniProtKB/TrEMBL
    Q53H10

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060927.1 Alternate T2T-CHM13v2.0

    Range
    8868283..8960543 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_054347265.1 → XP_054203240.1  E3 ubiquitin-protein ligase RAD18 isoform X2

  2. XM_054347266.1 → XP_054203241.1  E3 ubiquitin-protein ligase RAD18 isoform X1

Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q9NS91.2 GenPept UniProtKB/Swiss-Prot:Q9NS91

Gene LinkOut

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