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TAF3 TATA-box binding protein associated factor 3 [ Homo sapiens (human) ]

Gene ID: 83860, updated on 5-Mar-2024

Summary

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Official Symbol
TAF3provided by HGNC
Official Full Name
TATA-box binding protein associated factor 3provided by HGNC
Primary source
HGNC:HGNC:17303
See related
Ensembl:ENSG00000165632 MIM:606576; AllianceGenome:HGNC:17303
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
TAF140; TAFII140; TAFII-140
Summary
The highly conserved RNA polymerase II transcription factor TFIID (see TAF1; MIM 313650) comprises the TATA box-binding protein (TBP; MIM 600075) and a set of TBP-associated factors (TAFs), including TAF3. TAFs contribute to promoter recognition and selectivity and act as antiapoptotic factors (Gangloff et al., 2001 [PubMed 11438666]).[supplied by OMIM, May 2009]
Expression
Ubiquitous expression in testis (RPKM 5.8), ovary (RPKM 3.8) and 25 other tissues See more
Orthologs
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Genomic context

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Location:
10p14
Exon count:
7
Annotation release Status Assembly Chr Location
RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 10 NC_000010.11 (7818505..8016631)
RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 10 NC_060934.1 (7819254..8017393)
105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 10 NC_000010.10 (7860468..8058594)

Chromosome 10 - NC_000010.11Genomic Context describing neighboring genes Neighboring gene ReSE screen-validated silencer GRCh37_chr10:7809082-7809283 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 2981 Neighboring gene H3K27ac hESC enhancer GRCh37_chr10:7830681-7831224 Neighboring gene Kin17 DNA and RNA binding protein Neighboring gene uncharacterized LOC105376392 Neighboring gene ATP synthase F1 subunit gamma Neighboring gene H3K27ac hESC enhancer GRCh37_chr10:7860144-7860662 Neighboring gene H3K27ac hESC enhancer GRCh37_chr10:7860663-7861180 Neighboring gene Sharpr-MPRA regulatory region 6244 Neighboring gene ReSE screen-validated silencer GRCh37_chr10:8003953-8004117 Neighboring gene Sharpr-MPRA regulatory region 13617 Neighboring gene uncharacterized LOC105376394 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr10:8084925-8085676 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr10:8085677-8086428 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr10:8086674-8087217 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr10:8087218-8087760 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 2983 Neighboring gene OCT4-NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr10:8091667-8092510 Neighboring gene OCT4-NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr10:8092511-8093354 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr10:8093355-8094198 Neighboring gene GATA3 antisense RNA 1 Neighboring gene GATA binding protein 3

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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Related articles in PubMed

  1. H3K4me3 interactions with TAF3 regulate preinitiation complex assembly and selective gene activation. Lauberth SM, et al. Cell, 2013 Feb 28. PMID 23452851, Free PMC Article
  2. Control of embryonic stem cell lineage commitment by core promoter factor, TAF3. Liu Z, et al. Cell, 2011 Sep 2. PMID 21884934, Free PMC Article
  3. Switching of the core transcription machinery during myogenesis. Deato MD, et al. Genes Dev, 2007 Sep 1. PMID 17704303, Free PMC Article
  4. Genome wide association analysis of a founder population identified TAF3 as a gene for MCHC in humans. Pistis G, et al. PLoS One, 2013. PMID 23935956, Free PMC Article
  5. TATA binding protein associated factor 3 (TAF3) interacts with p53 and inhibits its function. Bereczki O, et al. BMC Mol Biol, 2008 Jun 12. PMID 18549481, Free PMC Article

See all (62) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Data show that transcription cofactor TAF3 is required for transcription of the alpha spectrin SPTA1 gene.
    Title: Genome wide association analysis of a founder population identified TAF3 as a gene for MCHC in humans.
  2. Study shows that H3K4me3-TAF3 interactions direct global TFIID recruitment to active genes, some of which are p53 targets. Further analyses show that H3K4me3 enhances p53-dependent transcription by stimulating preinitiation complex formation.
    Title: H3K4me3 interactions with TAF3 regulate preinitiation complex assembly and selective gene activation.
  3. Study shows that this TAF3 activity is critical for early lineage segregation during stem cell differentiation.
    Title: Control of embryonic stem cell lineage commitment by core promoter factor, TAF3.
  4. Observational study and genome-wide association study of gene-disease association. (HuGE Navigator)
    Title: Novel association strategy with copy number variation for identifying new risk Loci of human diseases.
  5. Observational study of gene-disease association. (HuGE Navigator)
    Title: Coeliac disease-associated risk variants in TNFAIP3 and REL implicate altered NF-kappaB signalling.
  6. Data identified TAF3 as an evolutionarily conserved negative regulator of p53 transcription activation function.
    Title: TATA binding protein associated factor 3 (TAF3) interacts with p53 and inhibits its function.
Submit: New GeneRIF Correction

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
Loci associated with N-glycosylation of human immunoglobulin G show pleiotropy with autoimmune diseases and haematological cancers.
EBI GWAS Catalog

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Genotypes

HIV-1 interactions

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Protein interactions

Protein Gene Interaction Pubs
Tat tat A novel function of LBP-1, restricting HIV-1 transcription at the level of elongation by preventing the binding of TFIID to the promoter, is suppressed by HIV-1 Tat, indicating Tat helps recruit TFIID to the HIV-1 LTR PubMed
tat HIV-1 Tat stabilizes the interaction of TFIIA with TFIID, and TFIIA and TFIID are required to reconstitute Tat-specific and TAR-dependent activation of HIV transcription in vitro PubMed
tat HIV-1 Tat binds, through amino acids 36-50, directly to the TBP subunit of the TFIID holoenzyme complex (which includes at least TFIID subunits p250, p125, p70, TBP, and p30), and increases the interaction of TFIID with the HIV-1 LTR promoter PubMed
tat Interaction of TFIID with the HIV-1 LTR, and therefore presumably HIV-1 Tat protein, is primarily dependent on the LTR TATA element and may also be stabilized or regulated by flanking E box motifs and basic helix-loop-helix proteins such as AP-4 and E47 PubMed
tat TATA binding protein (TBP)-associated factors (TAFs) in the TFIID complex are required for activation by transcription factors, but are dispensable for HIV-1 Tat LTR transactivation function PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Clone Names

  • MGC25063

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables RNA polymerase II general transcription initiation factor activity IC
Inferred by Curator
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables p53 binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein heterodimerization activity IEA
Inferred from Electronic Annotation
more info
  
enables transcription factor binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables transcription regulator inhibitor activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in RNA polymerase II preinitiation complex assembly IPI
Inferred from Physical Interaction
more info
 PubMed 
involved_in RNA polymerase II preinitiation complex assembly ISS
Inferred from Sequence or Structural Similarity
more info
  
involved_in mRNA transcription by RNA polymerase II IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in maintenance of protein location in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in negative regulation of DNA-binding transcription factor activity IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in negative regulation of transcription by RNA polymerase II IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in positive regulation of transcription by RNA polymerase II IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in positive regulation of transcription initiation by RNA polymerase II IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
located_in male germ cell nucleus IEA
Inferred from Electronic Annotation
more info
  
located_in nuclear membrane IDA
Inferred from Direct Assay
more info
  
located_in nucleoplasm IDA
Inferred from Direct Assay
more info
  
located_in nucleoplasm TAS
Traceable Author Statement
more info
  
located_in nucleus EXP
Inferred from Experiment
more info
 PubMed 
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus ISS
Inferred from Sequence or Structural Similarity
more info
  
part_of transcription factor TFIID complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of transcription factor TFIID complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of transcription factor TFIID complex IPI
Inferred from Physical Interaction
more info
 PubMed 
part_of transcription factor TFIID complex ISS
Inferred from Sequence or Structural Similarity
more info
  

General protein information

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Preferred Names
transcription initiation factor TFIID subunit 3
Names
140 kDa TATA box-binding protein-associated factor
RNA polymerase II transcription factor TAFII140
TAF(II)140
TAF3 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 140kDa
TBP-associated factor 3
transcription initiation factor TFIID 140 kDa subunit

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_031923.4 → NP_114129.1  transcription initiation factor TFIID subunit 3

    See identical proteins and their annotated locations for NP_114129.1

    Status: VALIDATED

    Source sequence(s)
    AL159172, AL353754, AL390294
    Consensus CDS
    CCDS41487.1
    UniProtKB/Swiss-Prot
    Q05DA0, Q5VWG9, Q6GMS5, Q6P6B5, Q86VY6, Q9BQS9, Q9UFI8
    Related
    ENSP00000340271.5, ENST00000344293.6
    Conserved Domains (4) summary
    smart00576
    Location:5 → 79
    BTP; Bromodomain transcription factors and PHD domain containing proteins
    PTZ00449
    Location:229 → 388
    PTZ00449; 104 kDa microneme/rhoptry antigen; Provisional
    PRK12323
    Location:777 → 847
    PRK12323; DNA polymerase III subunit gamma/tau
    cd15522
    Location:867 → 912
    PHD_TAF3; PHD finger found in transcription initiation factor TFIID subunit 3 (TAF3)

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000010.11 Reference GRCh38.p14 Primary Assembly

    Range
    7818505..8016631
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_011519741.2 → XP_011518043.2  transcription initiation factor TFIID subunit 3 isoform X1

    UniProtKB/TrEMBL
    A0A8I5KR98
    Related
    ENSP00000508875.1, ENST00000687522.1
    Conserved Domains (4) summary
    smart00576
    Location:5 → 79
    BTP; Bromodomain transcription factors and PHD domain containing proteins
    cd15522
    Location:866 → 911
    PHD_TAF3; PHD finger found in transcription initiation factor TFIID subunit 3 (TAF3)
    cl25751
    Location:228 → 387
    DUF4045; Domain of unknown function (DUF4045)
    cl26386
    Location:776 → 846
    DNA_pol3_gamma3; DNA polymerase III subunits gamma and tau domain III

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060934.1 Alternate T2T-CHM13v2.0

    Range
    7819254..8017393
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_054366902.1 → XP_054222877.1  transcription initiation factor TFIID subunit 3 isoform X1

Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q5VWG9.1 GenPept UniProtKB/Swiss-Prot:Q5VWG9

Gene LinkOut

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