Ppp1r9a protein phosphatase 1, regulatory subunit 9A [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Ppp1r9aprovided by RGD
Official Full Name: protein phosphatase 1, regulatory subunit 9Aprovided by RGD
Primary source: RGD:632280
See related: Ensembl:ENSRNOG00000008869 AllianceGenome:RGD:632280
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Neb1; p180; PP1bp175
Summary: Enables several functions, including enzyme binding activity; protein C-terminus binding activity; and transmembrane transporter binding activity. Involved in several processes, including modulation of chemical synaptic transmission; regulation of actin filament organization; and regulation of synapse organization. Acts upstream of or within neuron projection development. Located in several cellular components, including dendritic spine neck; growth cone lamellipodium; and postsynaptic density. Is active in glutamatergic synapse and postsynaptic actin cytoskeleton. Used to study transient cerebral ischemia. Biomarker of hepatocellular carcinoma. Orthologous to human PPP1R9A (protein phosphatase 1 regulatory subunit 9A). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Brain (RPKM 210.6), Testes (RPKM 72.0) and 7 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 4q21

Exon count:: 25

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	4	NC_086022.1 (33936949..34258927)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	4	NC_051339.1 (32970501..33292360)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	4	NC_005103.4 (29976485..30247371)

Chromosome 4 - NC_086022.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

PP1 bound on spinophilin, a close homolog of neurabin and another major synaptic PP1 anchoring protein, does not play a role in long term depression [LTD] induction, which suggests that neurabin plays a privileged role in nanodomain targeting of PP1 in LTD induction.
Title: Distinct Roles of Protein Phosphatase 1 Bound on Neurabin and Spinophilin and Its Regulation in AMPA Receptor Trafficking and LTD Induction.
Data show that the PP1:spinophilin holoenzyme is dynamic in solution, which allows for an increased capture radius of spinophilin and is likely important for its biological role.
Title: Flexibility in the PP1:spinophilin holoenzyme.
The spinophilin interactome was charaterized in subcellular fractions solubilized from adult rodent striatum.
Title: Identification and validation of novel spinophilin-associated proteins in rodent striatum using an enhanced ex vivo shotgun proteomics approach.
selective targeting of PP1gamma1 to F-actin by neurabins 1& 2 in intact cells requires both the canonical PP1-binding motif and an auxiliary PP1gamma1-selectivity determinant
Title: Selective targeting of the gamma1 isoform of protein phosphatase 1 to F-actin in intact cells requires multiple domains in spinophilin and neurabin.
Mutation of the Cdk5 phosphorylation site reduces the phenotypic consequences of Neurabin-I overexpression both in vitro and in vivo
Title: Neurabin-I is phosphorylated by Cdk5: implications for neuronal morphogenesis and cortical migration.
study determined the structure of the neurabin SAM domain by solution state nuclear magnetic resonance spectroscopy
Title: Structural characterization of the neurabin sterile alpha motif domain.
NrbI-targeted PP1, in response to distinct synaptic activities, regulates the synaptic trafficking of specific AMPAR subunits
Title: Differential regulation of AMPA receptor trafficking by neurabin-targeted synaptic protein phosphatase-1 in synaptic transmission and long-term depression in hippocampus.
recruit specific isoforms of protein phosphatase-1 catalytic subunits
Title: The neuronal actin-binding proteins, neurabin I and neurabin II, recruit specific isoforms of protein phosphatase-1 catalytic subunits.
Postsynaptic actin-bound neurabin-I-PP1 complex regulates synaptic transmission and bidirectional changes in hippocampal plasticity.
Title: Actin-associated neurabin-protein phosphatase-1 complex regulates hippocampal plasticity.
there is a novel PP1gamma1-selective interaction domain in neurabin that may allow for selective regulation and/or subcellular targeting of PP1 isoforms
Title: A protein phosphatase-1gamma1 isoform selectivity determinant in dendritic spine-associated neurabin.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

U66888 (e-PCR)
- UniSTS:181038

AW530668 (e-PCR)
- UniSTS:244553

BF393278 (e-PCR)
- UniSTS:244052

AU026172 (e-PCR)
- UniSTS:114542

NoName (e-PCR)
- UniSTS:532171

Ppp1r9a (e-PCR), detects polymorphism
- UniSTS:532171

RH143674 (e-PCR)
- UniSTS:232920

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables GTPase binding	IDA Inferred from Direct Assay more info	PubMed
enables actin filament binding	IBA Inferred from Biological aspect of Ancestor more info
enables actin filament binding	IDA Inferred from Direct Assay more info	PubMed
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein domain specific binding	IDA Inferred from Direct Assay more info	PubMed
enables protein kinase binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein phosphatase 1 binding	IDA Inferred from Direct Assay more info	PubMed
enables protein phosphatase 1 binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-containing complex binding	IPI Inferred from Physical Interaction more info	PubMed
enables transmembrane transporter binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in actin filament organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in actin filament organization	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within actin filament organization	ISO Inferred from Sequence Orthology more info
involved_in calcium-mediated signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in calcium-mediated signaling	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within calcium-mediated signaling	ISO Inferred from Sequence Orthology more info
involved_in cellular response to toxic substance	IDA Inferred from Direct Assay more info	PubMed
involved_in excitatory postsynaptic potential	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in modulation of chemical synaptic transmission	IEA Inferred from Electronic Annotation more info
involved_in modulation of chemical synaptic transmission	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of long-term synaptic potentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of spontaneous neurotransmitter secretion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of stress fiber assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nervous system development	IEA Inferred from Electronic Annotation more info
involved_in neuron development	IEP Inferred from Expression Pattern more info	PubMed
involved_in neuron projection development	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within neuron projection development	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of dendritic spine development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of long-term synaptic depression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of neuron projection development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in postsynaptic actin cytoskeleton organization	IDA Inferred from Direct Assay more info	PubMed
involved_in postsynaptic actin cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of actin filament polymerization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of dendritic spine morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of filopodium assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of synapse assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of synapse structural plasticity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in synapse organization	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in actin cytoskeleton	IBA Inferred from Biological aspect of Ancestor more info
located_in actin cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
located_in cortical actin cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in cortical actin cytoskeleton	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
is_active_in dendrite	IBA Inferred from Biological aspect of Ancestor more info
located_in dendrite	IDA Inferred from Direct Assay more info	PubMed
located_in dendritic spine	IDA Inferred from Direct Assay more info	PubMed
located_in dendritic spine	IEA Inferred from Electronic Annotation more info
located_in dendritic spine	ISO Inferred from Sequence Orthology more info
located_in dendritic spine neck	IDA Inferred from Direct Assay more info	PubMed
located_in filopodium	IDA Inferred from Direct Assay more info	PubMed
located_in filopodium	IEA Inferred from Electronic Annotation more info
located_in filopodium	ISO Inferred from Sequence Orthology more info
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
located_in growth cone	IDA Inferred from Direct Assay more info	PubMed
located_in growth cone lamellipodium	IDA Inferred from Direct Assay more info	PubMed
located_in lamellipodium	IDA Inferred from Direct Assay more info	PubMed
located_in neuromuscular junction	IDA Inferred from Direct Assay more info	PubMed
located_in neuronal cell body	IDA Inferred from Direct Assay more info	PubMed
is_active_in postsynaptic actin cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
is_active_in postsynaptic actin cytoskeleton	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in postsynaptic density	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: neurabin-1

Names: actin-binding protein neurabin; neurabin-I; neural tissue-specific F-actin-binding protein I; protein phosphatase 1, regulatory (inhibitor) subunit 9A

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001401497.1 → NP_001388426.1 neurabin-1 isoform 1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000004

Conserved Domains (4) summary

cd09512
Location:1208 → 1277

SAM_Neurabin-like; SAM domain of SAM_Neurabin-like subfamily

smart00228
Location:502 → 593

PDZ; Domain present in PSD-95, Dlg, and ZO-1/2

TIGR02168
Location:681 → 920

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

pfam17817
Location:435 → 498

PDZ_5; PDZ domain
NM_053473.3 → NP_445925.1 neurabin-1 isoform 2

See identical proteins and their annotated locations for NP_445925.1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000004

UniProtKB/Swiss-Prot

O35867

UniProtKB/TrEMBL

A6IDS9

Related

ENSRNOP00000011756.4, ENSRNOT00000011756.5

Conserved Domains (4) summary

cd09512
Location:983 → 1052

SAM_Neurabin-like; SAM domain of SAM_Neurabin-like subfamily

cd00992
Location:506 → 589

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

TIGR02168
Location:659 → 904

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

pfam17817
Location:435 → 498

PDZ_5; PDZ domain

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086022.1 Reference GRCr8

Range

33936949..34258927

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039108474.2 → XP_038964402.1 neurabin-1 isoform X1

Conserved Domains (4) summary

cd09512
Location:1208 → 1277

SAM_Neurabin-like; SAM domain of SAM_Neurabin-like subfamily

smart00228
Location:502 → 593

PDZ; Domain present in PSD-95, Dlg, and ZO-1/2

TIGR02168
Location:681 → 920

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

pfam17817
Location:435 → 498

PDZ_5; PDZ domain
XM_063286778.1 → XP_063142848.1 neurabin-1 isoform X10
XM_063286768.1 → XP_063142838.1 neurabin-1 isoform X2

UniProtKB/TrEMBL

A6IDS7
XM_063286775.1 → XP_063142845.1 neurabin-1 isoform X8
XM_063286770.1 → XP_063142840.1 neurabin-1 isoform X4
XM_063286772.1 → XP_063142842.1 neurabin-1 isoform X5

UniProtKB/TrEMBL

A0A8I5ZL80
XM_063286780.1 → XP_063142850.1 neurabin-1 isoform X11
XM_006236058.5 → XP_006236120.1 neurabin-1 isoform X1

See identical proteins and their annotated locations for XP_006236120.1

Conserved Domains (4) summary

cd09512
Location:1208 → 1277

SAM_Neurabin-like; SAM domain of SAM_Neurabin-like subfamily

smart00228
Location:502 → 593

PDZ; Domain present in PSD-95, Dlg, and ZO-1/2

TIGR02168
Location:681 → 920

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

pfam17817
Location:435 → 498

PDZ_5; PDZ domain
XM_039108475.2 → XP_038964403.1 neurabin-1 isoform X2

UniProtKB/TrEMBL

A6IDS7

Conserved Domains (5) summary

cd09512
Location:1200 → 1269

SAM_Neurabin-like; SAM domain of SAM_Neurabin-like subfamily

smart00228
Location:502 → 593

PDZ; Domain present in PSD-95, Dlg, and ZO-1/2

TIGR02168
Location:681 → 920

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

pfam09730
Location:606 → 752

BicD; Microtubule-associated protein Bicaudal-D

pfam17817
Location:435 → 498

PDZ_5; PDZ domain
XM_039108479.2 → XP_038964407.1 neurabin-1 isoform X7

Conserved Domains (5) summary

cd09512
Location:1005 → 1074

SAM_Neurabin-like; SAM domain of SAM_Neurabin-like subfamily

cd00992
Location:506 → 589

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

TIGR02168
Location:681 → 920

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

pfam09730
Location:606 → 752

BicD; Microtubule-associated protein Bicaudal-D

pfam17817
Location:435 → 498

PDZ_5; PDZ domain
XM_039108480.2 → XP_038964408.1 neurabin-1 isoform X8

Conserved Domains (5) summary

cd09512
Location:997 → 1066

SAM_Neurabin-like; SAM domain of SAM_Neurabin-like subfamily

cd00992
Location:506 → 589

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

TIGR02168
Location:681 → 920

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

pfam09730
Location:606 → 752

BicD; Microtubule-associated protein Bicaudal-D

pfam17817
Location:435 → 498

PDZ_5; PDZ domain
XM_039108476.2 → XP_038964404.1 neurabin-1 isoform X3

Conserved Domains (4) summary

cd09512
Location:1190 → 1259

SAM_Neurabin-like; SAM domain of SAM_Neurabin-like subfamily

smart00228
Location:502 → 593

PDZ; Domain present in PSD-95, Dlg, and ZO-1/2

COG1196
Location:704 → 819

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

pfam17817
Location:435 → 498

PDZ_5; PDZ domain
XM_039108477.2 → XP_038964405.1 neurabin-1 isoform X4

Conserved Domains (4) summary

cd09512
Location:1186 → 1255

SAM_Neurabin-like; SAM domain of SAM_Neurabin-like subfamily

smart00228
Location:502 → 593

PDZ; Domain present in PSD-95, Dlg, and ZO-1/2

TIGR02168
Location:659 → 898

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

pfam17817
Location:435 → 498

PDZ_5; PDZ domain
XM_039108481.2 → XP_038964409.1 neurabin-1 isoform X9

UniProtKB/Swiss-Prot

O35867

UniProtKB/TrEMBL

A6IDS9

Conserved Domains (4) summary

cd09512
Location:983 → 1052

SAM_Neurabin-like; SAM domain of SAM_Neurabin-like subfamily

cd00992
Location:506 → 589

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

TIGR02168
Location:659 → 904

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

pfam17817
Location:435 → 498

PDZ_5; PDZ domain
XM_063286773.1 → XP_063142843.1 neurabin-1 isoform X6
XM_063286779.1 → XP_063142849.1 neurabin-1 isoform X11
XM_017592928.3 → XP_017448417.1 neurabin-1 isoform X1

Conserved Domains (4) summary

cd09512
Location:1208 → 1277

SAM_Neurabin-like; SAM domain of SAM_Neurabin-like subfamily

smart00228
Location:502 → 593

PDZ; Domain present in PSD-95, Dlg, and ZO-1/2

TIGR02168
Location:681 → 920

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

pfam17817
Location:435 → 498

PDZ_5; PDZ domain
XM_063286771.1 → XP_063142841.1 neurabin-1 isoform X4
XM_063286777.1 → XP_063142847.1 neurabin-1 isoform X9

UniProtKB/Swiss-Prot

O35867

UniProtKB/TrEMBL

A6IDS9
XM_063286774.1 → XP_063142844.1 neurabin-1 isoform X6