PYK2 pyruvate kinase PYK2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: PYK2
Official Full Name: pyruvate kinase PYK2
Primary source: SGD:S000005874
Locus tag: YOR347C
See related: AllianceGenome:SGD:S000005874; FungiDB:YOR347C; VEuPathDB:YOR347C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables melatonin binding activity and pyruvate kinase activity. Involved in pyruvate metabolic process. Located in mitochondrion. Human ortholog(s) of this gene implicated in Gaucher's disease; congenital nonspherocytic hemolytic anemia; malaria; pyruvate kinase deficiency of red cells; and type 2 diabetes mellitus. Orthologous to human PKLR (pyruvate kinase L/R) and PKM (pyruvate kinase M1/2). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See PYK2 in Genome Data Viewer

Location:: chromosome: XV

Exon count:: 1

Sequence:: Chromosome: XV; NC_001147.6 (984942..986462, complement)

Chromosome XV - NC_001147.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001147.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Integrin alphaVbeta1-activated PYK2 promotes the progression of non-small-cell lung cancer via the STAT3-VGF axis.
Title: Integrin αVβ1-activated PYK2 promotes the progression of non-small-cell lung cancer via the STAT3-VGF axis.
Studies of Thr298Cys mutant pyruvate kinase (PK) active site conclude that residue Thr298 is not critical for PK activity but plays a role in the activation of the water molecule and affects the pK that modulates catalytic activity.
Title: Proton donor in yeast pyruvate kinase: chemical and kinetic properties of the active site Thr 298 to Cys mutant.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables magnesium ion binding	IEA Inferred from Electronic Annotation more info
enables melatonin binding	IDA Inferred from Direct Assay more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables potassium ion binding	IEA Inferred from Electronic Annotation more info
enables pyruvate kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables pyruvate kinase activity	IEA Inferred from Electronic Annotation more info
enables pyruvate kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in glycolytic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in glycolytic process	IEA Inferred from Electronic Annotation more info
involved_in monocarboxylic acid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in pyruvate metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: pyruvate kinase PYK2

NP_014992.3

EC 2.7.1.40

Pyruvate kinase; appears to be modulated by phosphorylation; transcription repressed by glucose, and Pyk2p may be active under low glycolytic flux; PYK2 has a paralog, CDC19, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001147.6 Reference assembly

Range

984942..986462 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183767.3 → NP_014992.3 TPA: pyruvate kinase PYK2 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014992.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W342, P52489

UniProtKB/TrEMBL

A6ZPH7, B3LK04, B5VSM3, C7GNS1, C8ZH98, G2WNK7, N1P3V3

Conserved Domains (1) summary

cd00288
Location:20 → 502

Pyruvate_Kinase; Pyruvate kinase (PK): Large allosteric enzyme that regulates glycolysis through binding of the substrate, phosphoenolpyruvate, and one or more allosteric effectors. Like other allosteric enzymes, PK has a high substrate affinity R state and a low ...