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    PRKCQ protein kinase C theta [ Homo sapiens (human) ]

    Gene ID: 5588, updated on 28-Oct-2024

    Summary

    Official Symbol
    PRKCQprovided by HGNC
    Official Full Name
    protein kinase C thetaprovided by HGNC
    Primary source
    HGNC:HGNC:9410
    See related
    Ensembl:ENSG00000065675 MIM:600448; AllianceGenome:HGNC:9410
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    PRKCT; nPKC-theta
    Summary
    Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. [provided by RefSeq, Jul 2008]
    Expression
    Broad expression in thyroid (RPKM 4.0), lymph node (RPKM 2.9) and 17 other tissues See more
    Orthologs
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    See PRKCQ in Genome Data Viewer
    Location:
    10p15.1
    Exon count:
    21
    Annotation release Status Assembly Chr Location
    RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 10 NC_000010.11 (6394097..6580646, complement)
    RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 10 NC_060934.1 (6394273..6580768, complement)
    RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 10 NC_000010.10 (6436059..6622608, complement)

    Chromosome 10 - NC_000010.11Genomic Context describing neighboring genes Neighboring gene long intergenic non-protein coding RNA 2649 Neighboring gene CRISPRi-FlowFISH-validated PFKFB3 regulatory element GRCh37_chr10:6389972-6390472 Neighboring gene developmental pluripotency associated 5 pseudogene 3 Neighboring gene long intergenic non-protein coding RNA 2656 Neighboring gene uncharacterized LOC124902370 Neighboring gene NANOG hESC enhancer GRCh37_chr10:6452056-6452557 Neighboring gene Neanderthal introgressed variant-containing enhancer experimental_13978 Neighboring gene Neanderthal introgressed variant-containing enhancer experimental_14068 Neighboring gene Neanderthal introgressed variant-containing enhancer experimental_14123 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 2968 Neighboring gene uncharacterized LOC107984202 Neighboring gene Neanderthal introgressed variant-containing enhancer experimental_14215 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 2108 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 2109 Neighboring gene Sharpr-MPRA regulatory region 12587 Neighboring gene PRKCQ antisense RNA 1 Neighboring gene OCT4-NANOG-H3K27ac hESC enhancer GRCh37_chr10:6671711-6672282 Neighboring gene long intergenic non-protein coding RNA 2648 Neighboring gene OCT4-NANOG hESC enhancer GRCh37_chr10:6690794-6691459 Neighboring gene NANOG hESC enhancer GRCh37_chr10:6720092-6720593 Neighboring gene MED14-independent group 3 enhancer GRCh37_chr10:6778731-6779930 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr10:6779977-6780522 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr10:6780523-6781066 Neighboring gene lncRNA in non-homologous end joining pathway 1

    Genomic regions, transcripts, and products

    Expression

    • Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?

    Phenotypes

    EBI GWAS Catalog

    Description
    A genome-wide association study of bronchodilator response in asthmatics.
    EBI GWAS Catalog
    Common variants at CD40 and other loci confer risk of rheumatoid arthritis.
    EBI GWAS Catalog
    Genetics of rheumatoid arthritis contributes to biology and drug discovery.
    EBI GWAS Catalog
    Genome-wide association study and meta-analysis find that over 40 loci affect risk of type 1 diabetes.
    EBI GWAS Catalog
    Genome-wide association study meta-analysis identifies seven new rheumatoid arthritis risk loci.
    EBI GWAS Catalog
    Genome-wide association study of antibody response to smallpox vaccine.
    EBI GWAS Catalog
    Genome-wide association study of body mass index in 23 000 individuals with and without asthma.
    EBI GWAS Catalog
    Genome-wide association study of celiac disease in North America confirms FRMD4B as new celiac locus.
    EBI GWAS Catalog
    Meta-analysis of genome-wide association study data identifies additional type 1 diabetes risk loci.
    EBI GWAS Catalog

    HIV-1 interactions

    Protein interactions

    Protein Gene Interaction Pubs
    Envelope surface glycoprotein gp120 env HIV-1 gp120 activates forward trafficking and surface clustering of NMDA receptors in membrane micro domains by a PKA-dependent phosphorylation of the NR1 C-terminal Ser897, followed by a PKC-dependent phosphorylation of Ser896 PubMed
    env Down modulation of the interaction between HIV-1 gp120 and CD4 by TPA is blocked by protein kinase C (PKC) inhibitors, suggesting PKC may play an important role in HIV-1 infection PubMed
    env c-FLIPL inhibits Bax activation via modulating PKC expression at the transcriptional level involving AP-2 during gp120 treatment PubMed
    env Pre-treatment of endothelial cells with fibroblast growth factor 2 (FGF2) protects cells from HIV-1 gp120 angiotoxicity; this protection is regulated by crosstalk among the ERK, PI3K-AKT and PKC signaling pathways PubMed
    env Induction of apoptosis in cell cultures through binding of HIV-1 gp120 or gp160 to CXCR4 involves protein kinase C, basic fibroblast growth factor, caspase-3, and the pro-apoptotic molecule Bax PubMed
    env HIV-1 gp120 increases the levels of calcium-dependent and -independent PKC isozymes; the most striking change is observed in PKC-zeta isozyme levels PubMed
    env A specific interaction between CD4 and HIV-1 gp120 is required for phosphorylation of CD4, which could involve protein kinase C PubMed
    env IL-16 induces rapid translocation of PKC from the cytosol to the membrane in CD4+ cells; PKC inhibitors completely block IL-16-induced lymphocyte migration as well as the motile response induced by HIV-1 gp120 and anti-CD4 antibody binding to CD4 PubMed
    Envelope surface glycoprotein gp160, precursor env HIV-1 gp160-induced AP-1 complex formation is dependent upon protein tyrosine phosphorylation and is abolished by inhibitors of protein kinase C, but it is unaffected by calcium channel blockers or cyclosporine A PubMed
    Envelope transmembrane glycoprotein gp41 env A synthetic peptide corresponding to cytoplasmic domain residues 828-848 of HIV-1 gp41 inhibits pKC-catalysed phosphorylation of a protein substrate PubMed
    env A synthetic peptide containing residues 581-597 from HIV-1 gp41 inhibits protein kinase C (pkC)-mediated phosphorylation of the CD3 gamma-chain in intact cells and directly inhibits partially purified pkC PubMed
    Nef nef PKCtheta is a downstream effector of LCK and is recruited by HIV-1 Nef to form the Nef/LCK/PKCtheta complex, which promotes HIV transcription PubMed
    nef The formation of Nef/LCK/PKCtheta complex activates the ERK MAPK signaling pathway PubMed
    nef Interactions between HIV-1 Nef and PKC isozymes in Jurkat cells show that Nef interacts preferentially with PKCtheta; the interaction of Nef and PKCtheta is independent of calcium, but enhanced by phospholipid activators of PKC PubMed
    Tat tat HIV-1 Tat relocalizes PRKCQ (PKC-theta) and RELA (NFkB) to the nucleus of Jurkat T cells that stably express Tat PubMed
    tat HIV-1 Tat induces phosphorylation of Threonine538 in PRKCQ (PKC-theta) in Jurkat T cells stably expressing Tat PubMed
    tat HIV-1 Tat activates PRKCQ (PKC-theta) kinase activity, which leads to RELA (NFkB), NRAS, RAF1, MAP2K1 (MEK1), MAP2K2 (MEK2), MAPK3 (ERK1), and MAPK1 (ERK2) activation, in Jurkat T cells that stably express Tat PubMed
    tat HIV-1 Tat upregulates PRKCQ (PKC-theta) expression in Jurkat T cells stably expressing Tat PubMed
    tat HIV-1 Tat activates protein kinase C, resulting in the induction of TNF-alpha, IL-6 and IL-10 expression and the secretion of MCP-1 PubMed
    tat Tat-triggered PKCdelta and PKCtheta activation results in the downstream signaling through the apoptosis pathways mediated by both ERK1/2 and caspase-3 PubMed
    tat Phospholipase C/protein kinase C signaling pathway-dependent phosphorylation of p44/42 and JNK MAP kinases participates partially in IL-1beta induction by TAT PubMed
    tat Protein kinase C is required for HIV-1 Tat-mediated transactivation of the viral LTR promoter, indicating protein kinase C regulates the process of HIV-1 transactivation and may play a role in the transition of HIV from latency to productive growth PubMed
    tat Protein kinase C phosphorylates HIV-1 Tat on serine residue 46 PubMed
    matrix gag Protein kinase C (PKC) phosphorylates HIV-1 Matrix on serine residue 111 resulting in a shift in localization of Matrix from the cytosol to the cellular membrane, suggesting a myristoyl-protein switch regulated by PKC phosphorylation PubMed
    retropepsin gag-pol Phosphorylation of human recombinant vimentin by PKC inhibits the proteolytic processing of the vimentin head domain by HIV-1 protease PubMed
    reverse transcriptase gag-pol HIV-1 RT heterodimer expressed in bacteria can be phosphorylated in vitro by several purified mammalian protein kinases including auto-activated protein kinase (PK), CKII, cytosolic protamine kinase (CPK), myelin basic protein kinase 1 (MBPK1), and PRKC PubMed

    Go to the HIV-1, Human Interaction Database

    Pathways from PubChem

    Interactions

    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Markers

    Clone Names

    • MGC126514, MGC141919

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
     
    enables diacylglycerol-dependent serine/threonine kinase activity EXP
    Inferred from Experiment
    more info
    PubMed 
    enables diacylglycerol-dependent serine/threonine kinase activity TAS
    Traceable Author Statement
    more info
     
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
     
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    enables protein kinase activity IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables protein serine kinase activity IEA
    Inferred from Electronic Annotation
    more info
     
    enables protein serine/threonine kinase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    enables protein serine/threonine kinase activity NAS
    Non-traceable Author Statement
    more info
    PubMed 
    Process Evidence Code Pubs
    involved_in CD4-positive, alpha-beta T cell proliferation IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in Fc-epsilon receptor signaling pathway TAS
    Traceable Author Statement
    more info
     
    involved_in axon guidance TAS
    Traceable Author Statement
    more info
     
    involved_in cell chemotaxis IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in inflammatory response IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in intracellular signal transduction IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    involved_in intracellular signal transduction NAS
    Non-traceable Author Statement
    more info
    PubMed 
    involved_in membrane protein ectodomain proteolysis ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    involved_in negative regulation of T cell apoptotic process IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in negative regulation of insulin receptor signaling pathway IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in positive regulation of CD4-positive, alpha-beta T cell proliferation IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in positive regulation of NF-kappaB transcription factor activity IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in positive regulation of T cell activation ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    involved_in positive regulation of T-helper 17 type immune response ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    involved_in positive regulation of T-helper 2 cell activation ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    involved_in positive regulation of interleukin-17 production ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    involved_in positive regulation of interleukin-2 production IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in positive regulation of interleukin-4 production ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    acts_upstream_of_or_within positive regulation of telomere maintenance IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in protein phosphorylation IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in regulation of DNA-templated transcription ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    involved_in regulation of cell growth NAS
    Non-traceable Author Statement
    more info
    PubMed 
    involved_in regulation of platelet aggregation ISS
    Inferred from Sequence or Structural Similarity
    more info
     
    Component Evidence Code Pubs
    located_in aggresome IDA
    Inferred from Direct Assay
    more info
     
    located_in centriolar satellite IDA
    Inferred from Direct Assay
    more info
     
    located_in cytosol TAS
    Traceable Author Statement
    more info
     
    located_in immunological synapse IEA
    Inferred from Electronic Annotation
    more info
     
    located_in plasma membrane TAS
    Traceable Author Statement
    more info
     

    General protein information

    Preferred Names
    protein kinase C theta type
    NP_001229342.1
    NP_001269573.1
    NP_001269574.1
    NP_001310194.1
    NP_001310195.1
    NP_001310196.1
    NP_006248.1
    XP_005252553.1
    XP_005252554.1
    XP_054222269.1
    XP_054222270.1

    NCBI Reference Sequences (RefSeq)

    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001242413.2NP_001229342.1  protein kinase C theta type isoform 2

      See identical proteins and their annotated locations for NP_001229342.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (2) lacks an in-frame exon in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.
      Source sequence(s)
      AA234440, AK293935, AK308099, AK312411, AY702977, BX647657, L01087, R40214
      Consensus CDS
      CCDS55701.1
      UniProtKB/Swiss-Prot
      Q04759
      Related
      ENSP00000380361.2, ENST00000397176.6
      Conserved Domains (3) summary
      PTZ00263
      Location:376631
      PTZ00263; protein kinase A catalytic subunit; Provisional
      pfam00130
      Location:232284
      C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
      cl21453
      Location:374641
      PKc_like; Protein Kinases, catalytic domain
    2. NM_001282644.2NP_001269573.1  protein kinase C theta type isoform 3

      Status: VALIDATED

      Description
      Transcript Variant: This variant (3) uses an alternate splice site in the 5' region. This difference results in translation initiation at an alternate AUG and results in an isoform (3) with a shorter and distinct N-terminus, compared to isoform 1.
      Source sequence(s)
      AA234440, AK308099, BX647657, R40214
      UniProtKB/Swiss-Prot
      Q04759
      Conserved Domains (3) summary
      smart00220
      Location:344598
      S_TKc; Serine/Threonine protein kinases, catalytic domain
      cd05619
      Location:338668
      STKc_nPKC_theta; Catalytic domain of the Serine/Threonine Kinase, Novel Protein Kinase C theta
      pfam00130
      Location:196248
      C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
    3. NM_001282645.1NP_001269574.1  protein kinase C theta type isoform 4

      See identical proteins and their annotated locations for NP_001269574.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (4) lacks an exon in the 5' region. This difference results in translation initiation at an alternate AUG and results in an isoform (4) with a shorter and distinct N-terminus, compared to isoform 1.
      Source sequence(s)
      AA234440, AK293935, AK308099, BX647657, L01087, R40214
      Consensus CDS
      CCDS60482.1
      UniProtKB/Swiss-Prot
      Q04759
      Related
      ENSP00000441752.1, ENST00000539722.5
      Conserved Domains (2) summary
      cd05619
      Location:249579
      STKc_nPKC_theta; Catalytic domain of the Serine/Threonine Kinase, Novel Protein Kinase C theta
      pfam00130
      Location:107159
      C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
    4. NM_001323265.1NP_001310194.1  protein kinase C theta type isoform 1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (5) and variant 1 both encode isoform 1.
      Source sequence(s)
      AL137145, AL158043
      Consensus CDS
      CCDS7079.1
      UniProtKB/Swiss-Prot
      B4DF52, Q04759, Q14DH6, Q3MJF1, Q64FY5, Q9H508, Q9H549
      Conserved Domains (2) summary
      cd05619
      Location:374704
      STKc_nPKC_theta; Catalytic domain of the Serine/Threonine Kinase, Novel Protein Kinase C theta
      pfam00130
      Location:232284
      C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
    5. NM_001323266.2NP_001310195.1  protein kinase C theta type isoform 4

      Status: VALIDATED

      Description
      Transcript Variant: This variant (6) and variant 4 both encode isoform 4.
      Source sequence(s)
      AL137145, AL158043
      Consensus CDS
      CCDS60482.1
      UniProtKB/Swiss-Prot
      Q04759
      Conserved Domains (2) summary
      cd05619
      Location:249579
      STKc_nPKC_theta; Catalytic domain of the Serine/Threonine Kinase, Novel Protein Kinase C theta
      pfam00130
      Location:107159
      C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
    6. NM_001323267.2NP_001310196.1  protein kinase C theta type isoform 5

      Status: VALIDATED

      Source sequence(s)
      AL137145, AL158043
    7. NM_006257.5NP_006248.1  protein kinase C theta type isoform 1

      See identical proteins and their annotated locations for NP_006248.1

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) encodes the longest isoform (1). Variants 1 and 5 both encode the same isoform (1).
      Source sequence(s)
      AA234440, AK293935, AK308099, AK312411, BX647657, R40214
      Consensus CDS
      CCDS7079.1
      UniProtKB/Swiss-Prot
      B4DF52, Q04759, Q14DH6, Q3MJF1, Q64FY5, Q9H508, Q9H549
      Related
      ENSP00000263125.5, ENST00000263125.10
      Conserved Domains (2) summary
      cd05619
      Location:374704
      STKc_nPKC_theta; Catalytic domain of the Serine/Threonine Kinase, Novel Protein Kinase C theta
      pfam00130
      Location:232284
      C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000010.11 Reference GRCh38.p14 Primary Assembly

      Range
      6394097..6580646 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_005252497.5XP_005252554.1  protein kinase C theta type isoform X2

      Conserved Domains (3) summary
      smart00220
      Location:414668
      S_TKc; Serine/Threonine protein kinases, catalytic domain
      pfam00130
      Location:266318
      C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
      cl21453
      Location:408695
      PKc_like; Protein Kinases, catalytic domain
    2. XM_005252496.5XP_005252553.1  protein kinase C theta type isoform X1

      Conserved Domains (3) summary
      smart00220
      Location:414668
      S_TKc; Serine/Threonine protein kinases, catalytic domain
      cd05619
      Location:408738
      STKc_nPKC_theta; Catalytic domain of the Serine/Threonine Kinase, Novel Protein Kinase C theta
      pfam00130
      Location:266318
      C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060934.1 Alternate T2T-CHM13v2.0

      Range
      6394273..6580768 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_054366295.1XP_054222270.1  protein kinase C theta type isoform X2

    2. XM_054366294.1XP_054222269.1  protein kinase C theta type isoform X1