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    TRIM45 tripartite motif containing 45 [ Homo sapiens (human) ]

    Gene ID: 80263, updated on 28-Oct-2024

    Summary

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    Official Symbol
    TRIM45provided by HGNC
    Official Full Name
    tripartite motif containing 45provided by HGNC
    Primary source
    HGNC:HGNC:19018
    See related
    Ensembl:ENSG00000134253 MIM:609318; AllianceGenome:HGNC:19018
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    RNF99
    Summary
    Enables ubiquitin protein ligase activity. Involved in negative regulation of inflammatory response; proteasome-mediated ubiquitin-dependent protein catabolic process; and protein K48-linked ubiquitination. Located in cytosol; intercellular bridge; and nucleoplasm. Is active in cytoplasm. [provided by Alliance of Genome Resources, Oct 2024]
    Expression
    Ubiquitous expression in endometrium (RPKM 2.4), thyroid (RPKM 2.2) and 24 other tissues See more
    Orthologs
    mouse all
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    Genomic context

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    See TRIM45 in Genome Data Viewer
    Location:
    1p13.1
    Exon count:
    8
    Annotation release Status Assembly Chr Location
    RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 1 NC_000001.11 (117111060..117123927, complement)
    RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 1 NC_060925.1 (117121483..117134348, complement)
    RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 1 NC_000001.10 (117653682..117664371, complement)

    Chromosome 1 - NC_000001.11Genomic Context describing neighboring genes Neighboring gene CD101 molecule Neighboring gene CD101 antisense RNA 1 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 1578 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 1236 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 1579 Neighboring gene CDK7 strongly-dependent group 2 enhancer GRCh37_chr1:117619501-117620700 Neighboring gene transcription termination factor 2 Neighboring gene microRNA 942 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 1237 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 1580 Neighboring gene uncharacterized LOC124904387 Neighboring gene MPRA-validated peak386 silencer Neighboring gene CDK7 strongly-dependent group 2 enhancer GRCh37_chr1:117689065-117690264 Neighboring gene ribosomal protein S15a pseudogene 9 Neighboring gene V-set domain containing T cell activation inhibitor 1 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr1:117714175-117714737 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr1:117714738-117715299

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

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    Related articles in PubMed

    1. TRIM45 negatively regulates NF-κB-mediated transcription and suppresses cell proliferation. Shibata M, et al. Biochem Biophys Res Commun, 2012 Jun 22. PMID 22634006
    2. TRIM45 Suppresses the Development of Non-small Cell Lung Cancer. Peng X, et al. Curr Mol Med, 2020. PMID 31663468
    3. TRIM45 facilitates NASH-progressed HCC by promoting fatty acid synthesis via catalyzing FABP5 ubiquitylation. Li X, et al. Oncogene, 2024 Jun. PMID 38755308
    4. TRIM45, a novel human RBCC/TRIM protein, inhibits transcriptional activities of ElK-1 and AP-1. Wang Y, et al. Biochem Biophys Res Commun, 2004 Oct 8. PMID 15351693
    5. TRIM45 functions as a tumor suppressor in the brain via its E3 ligase activity by stabilizing p53 through K63-linked ubiquitination. Zhang J, et al. Cell Death Dis, 2017 May 25. PMID 28542145, Free PMC Article

    See all (27) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. TRIM45 facilitates NASH-progressed HCC by promoting fatty acid synthesis via catalyzing FABP5 ubiquitylation.
      Title: TRIM45 facilitates NASH-progressed HCC by promoting fatty acid synthesis via catalyzing FABP5 ubiquitylation.
    2. TRIM45 aggravates microglia pyroptosis via Atg5/NLRP3 axis in septic encephalopathy.
      Title: TRIM45 aggravates microglia pyroptosis via Atg5/NLRP3 axis in septic encephalopathy.
    3. TRIM45 Suppresses the Development of Non-small Cell Lung Cancer.
      Title: TRIM45 Suppresses the Development of Non-small Cell Lung Cancer.
    4. These findings suggest that TRIM45 is a novel tumor suppressor that stabilizes and activates p53 in glioma.
      Title: TRIM45 functions as a tumor suppressor in the brain via its E3 ligase activity by stabilizing p53 through K63-linked ubiquitination.
    5. The TRIM-FLMN protein TRIM45 directly interacts with RACK1 and negatively regulates PKC-mediated signaling pathway.
      Title: The TRIM-FLMN protein TRIM45 directly interacts with RACK1 and negatively regulates PKC-mediated signaling pathway.
    6. These findings suggest that TRIM45 acts as a repressor for the NF-kappaB signal and regulates cell growth.
      Title: TRIM45 negatively regulates NF-κB-mediated transcription and suppresses cell proliferation.
    7. TRIM45 may act as a new transcriptional repressor in mitogen-activated protein kinase signaling pathway
      Title: TRIM45, a novel human RBCC/TRIM protein, inhibits transcriptional activities of ElK-1 and AP-1.
    Submit: New GeneRIF Correction

    Phenotypes

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    Find tests for this gene in the NIH Genetic Testing Registry (GTR)

    Review eQTL and phenotype association data in this region using PheGenI

    Variation

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    See variants in ClinVar

    See studies and variants in dbVar

    See Variation Viewer (GRCh37.p13)

    See Variation Viewer (GRCh38)

    Genotypes

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Clone Names

    • FLJ13181

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables ubiquitin protein ligase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables ubiquitin protein ligase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables zinc ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in bone development IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in negative regulation of inflammatory response IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in proteasome-mediated ubiquitin-dependent protein catabolic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in protein K48-linked ubiquitination IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    is_active_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		  
    located_in intercellular bridge IDA
    Inferred from Direct Assay
    more info
    		  
    is_active_in nucleoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in nucleoplasm IDA
    Inferred from Direct Assay
    more info
    		  

    General protein information

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    Preferred Names
    E3 ubiquitin-protein ligase TRIM45
    Names
    ring finger protein 99
    NP_001139107.1
    NP_079464.2
    XP_047286870.1
    XP_054194842.1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    Genomic

    1. NG_028008.1 RefSeqGene

      Range
      5041..15730
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001145635.2 → NP_001139107.1  E3 ubiquitin-protein ligase TRIM45 isoform 2

      See identical proteins and their annotated locations for NP_001139107.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.
      Source sequence(s)
      AK222901, AY669488, BC034943, BU165629, EC541819
      Consensus CDS
      CCDS44200.1
      UniProtKB/TrEMBL
      Q5T2K3
      Related
      ENSP00000358476.3, ENST00000369464.7
      Conserved Domains (4) summary
      smart00557
      Location:391 → 479
      IG_FLMN; Filamin-type immunoglobulin domains
      cd00021
      Location:189 → 226
      BBOX; B-Box-type zinc finger; zinc binding domain (CHC3H2); often present in combination with other motifs, like RING zinc finger, NHL motif, coiled-coil or RFP domain in functionally unrelated proteins, most likely mediating protein-protein interaction.
      pfam00630
      Location:391 → 476
      Filamin; Filamin/ABP280 repeat
      cl23765
      Location:240 → 357
      iSH2_PI3K_IA_R; Inter-Src homology 2 (iSH2) helical domain of Class IA Phosphoinositide 3-kinase Regulatory subunits

    2. NM_025188.4 → NP_079464.2  E3 ubiquitin-protein ligase TRIM45 isoform 1

      See identical proteins and their annotated locations for NP_079464.2

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
      Source sequence(s)
      AK222901, AY669488, BU165629, EC541819
      Consensus CDS
      CCDS893.1
      UniProtKB/Swiss-Prot
      Q53GN0, Q5T2K4, Q5T2K5, Q8IYV6, Q9H8W5
      UniProtKB/TrEMBL
      Q5T2K3
      Related
      ENSP00000256649.4, ENST00000256649.9
      Conserved Domains (4) summary
      smart00557
      Location:399 → 497
      IG_FLMN; Filamin-type immunoglobulin domains
      cd00021
      Location:189 → 226
      BBOX; B-Box-type zinc finger; zinc binding domain (CHC3H2); often present in combination with other motifs, like RING zinc finger, NHL motif, coiled-coil or RFP domain in functionally unrelated proteins, most likely mediating protein-protein interaction.
      pfam00630
      Location:397 → 494
      Filamin; Filamin/ABP280 repeat
      cl23765
      Location:240 → 357
      iSH2_PI3K_IA_R; Inter-Src homology 2 (iSH2) helical domain of Class IA Phosphoinositide 3-kinase Regulatory subunits

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000001.11 Reference GRCh38.p14 Primary Assembly

      Range
      117111060..117123927 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_047430914.1 → XP_047286870.1  E3 ubiquitin-protein ligase TRIM45 isoform X1

      Related
      ENSP00000358473.3, ENST00000369461.3

    RNA

    1. XR_007063980.1 RNA Sequence

    2. XR_246297.2 RNA Sequence

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060925.1 Alternate T2T-CHM13v2.0

      Range
      117121483..117134348 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_054338867.1 → XP_054194842.1  E3 ubiquitin-protein ligase TRIM45 isoform X1

    RNA

    1. XR_008486169.1 RNA Sequence

    2. XR_008486168.1 RNA Sequence

    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9H8W5.2 GenPept UniProtKB/Swiss-Prot:Q9H8W5

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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