UBE2I ubiquitin conjugating enzyme E2 I [Homo sapiens (human)] - Gene

Summary

Official Symbol: UBE2Iprovided by HGNC
Official Full Name: ubiquitin conjugating enzyme E2 Iprovided by HGNC
Primary source: HGNC:HGNC:12485
See related: Ensembl:ENSG00000103275 MIM:601661; AllianceGenome:HGNC:12485
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: P18; UBC9; C358B7.1
Summary: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Expression: Ubiquitous expression in ovary (RPKM 23.2), endometrium (RPKM 17.8) and 25 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 16p13.3

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCh38.p14 (GCF_000001405.40)	16	NC_000016.10 (1309152..1327017)
RS_2024_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	16	NC_060940.1 (1325332..1343194)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	16	NC_000016.9 (1359153..1377018)

Chromosome 16 - NC_000016.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Repression of the SUMO-conjugating enzyme UBC9 is associated with lowered double minutes and reduced tumor progression.
Title: Repression of the SUMO-conjugating enzyme UBC9 is associated with lowered double minutes and reduced tumor progression.
The Interaction between SARS-CoV-2 Nucleocapsid Protein and UBC9 Inhibits MAVS Ubiquitination by Enhancing Its SUMOylation.
Title: The Interaction between SARS-CoV-2 Nucleocapsid Protein and UBC9 Inhibits MAVS Ubiquitination by Enhancing Its SUMOylation.
Stabilization of Pin1 by USP34 promotes Ubc9 isomerization and protein sumoylation in glioma stem cells.
Title: Stabilization of Pin1 by USP34 promotes Ubc9 isomerization and protein sumoylation in glioma stem cells.
Knockdown of UBE2I inhibits tumorigenesis and enhances chemosensitivity of cholangiocarcinoma via modulating p27kip1 nuclear export.
Title: Knockdown of UBE2I inhibits tumorigenesis and enhances chemosensitivity of cholangiocarcinoma via modulating p27kip1 nuclear export.
Upregulation of miRNA-10a-5p promotes tumor progression in cervical cancer by suppressing UBE2I signaling.
Title: Upregulation of miRNA-10a-5p promotes tumor progression in cervical cancer by suppressing UBE2I signaling.
Combined Evaluation of mRNA and Protein Expression, Promoter Methylation, and Immune Infiltration of UBE2I in Pan-Digestive System Tumors.
Title: Combined Evaluation of mRNA and Protein Expression, Promoter Methylation, and Immune Infiltration of UBE2I in Pan-Digestive System Tumors.
SUMOylation stabilizes hSSB1 and enhances the recruitment of NBS1 to DNA damage sites.
Title: SUMOylation stabilizes hSSB1 and enhances the recruitment of NBS1 to DNA damage sites.
Adipose-specific knockout of ubiquitin-conjugating enzyme E2L6 (Ube2l6) reduces diet-induced obesity, insulin resistance, and hepatic steatosis.
Title: Adipose-specific knockout of ubiquitin-conjugating enzyme E2L6 (Ube2l6) reduces diet-induced obesity, insulin resistance, and hepatic steatosis.
HRM screening of the UBC9 gene encoding the SUMO-E2-conjugating enzyme - case-control study in breast cancer.
Title: HRM screening of the UBC9 gene encoding the SUMO-E2-conjugating enzyme - case-control study in breast cancer.
UBC9 coordinates inflammation affecting development of bladder cancer.
Title: UBC9 coordinates inflammation affecting development of bladder cancer.

Submit: New GeneRIF Correction See all GeneRIFs (125)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Protein interactions

Protein	Gene	Interaction	Pubs
Envelope surface glycoprotein gp120	env	Levels of HIV-1 gp120 and Endo Hf-resistant gp120 are reduced in the absence of UBC9 expression. Degradation of gp120 occurs before transport to the plasma membrane and the lipid rafts in the UBC9 knockdown cells	PubMed
	env	UBC9 contributes to viral glycoproteins gp120/gp41 stability in the presence of Gag expression and affects incorporation of the gp120/gp41 into virions	PubMed
Envelope transmembrane glycoprotein gp41	env	UBC9 contributes to viral glycoproteins gp120/gp41 stability in the presence of Gag expression and affects incorporation of the gp120/gp41 into released virions	PubMed
Pr55(Gag)	gag	HIV-1 Gag trafficking and association with the plasma membrane is altered in the absebce of UBC9	PubMed
	gag	UBC9 contributes to viral glycoproteins gp120/gp41 stability in the presence of Gag expression and affects incorporation of the gp120/gp41 into released virions	PubMed
	gag	Disrupting the function of KIF4 leads Gag transient accumulation in discrete, perinuclear, nonendocytic clusters that co-localize with endogenous KIF4, Ubc9, and SUMO	PubMed
	gag	Coimmunoprecipitation shows that HIV-1 Gag and human UBC9 interact in vivo. HIV-1 Gag and human UBC9 colocalize in HIV-1-expressing cells	PubMed
integrase	gag-pol	HIV-1 IN interacts with SUMO1/SUMO2 and Ubc9 in cells. Upregulations of SUMO1/SUMO2 and Ubc9 inhibit HIV-1 integration, while downregulations of SUMO1/SUMO2 and Ubc9 increase HIV-1 integration	PubMed
p6	gag	HIV-1 p6 interacts with Ubc9 in a yeast two-hybrid system	PubMed

Go to the HIV-1, Human Interaction Database

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH79139 (e-PCR)
- UniSTS:19458

UBE2I_3883 (e-PCR)
- UniSTS:462608

RH70600 (e-PCR)
- UniSTS:35364

SHGC-60704 (e-PCR)
- UniSTS:6269

G32807 (e-PCR)
- UniSTS:117386

SHGC-60764 (e-PCR)
- UniSTS:55209

SHGC-61088 (e-PCR)
- UniSTS:68785

A009S20 (e-PCR)
- UniSTS:73167

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables RING-like zinc finger domain binding	IPI Inferred from Physical Interaction more info	PubMed
enables RNA binding	HDA more info	PubMed
enables SUMO conjugating enzyme activity	IBA Inferred from Biological aspect of Ancestor more info
enables SUMO conjugating enzyme activity	IDA Inferred from Direct Assay more info	PubMed
enables SUMO transferase activity	EXP Inferred from Experiment more info	PubMed
enables SUMO transferase activity	TAS Traceable Author Statement more info
enables enzyme binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables small protein activating enzyme binding	IPI Inferred from Physical Interaction more info	PubMed
enables transcription coregulator binding	IPI Inferred from Physical Interaction more info	PubMed
enables transcription factor binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in chromosome segregation	IEA Inferred from Electronic Annotation more info
involved_in mitotic nuclear membrane reassembly	TAS Traceable Author Statement more info
involved_in negative regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	TAS Traceable Author Statement more info
involved_in nuclear export	EXP Inferred from Experiment more info	PubMed
involved_in positive regulation of SUMO transferase activity	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein modification process	TAS Traceable Author Statement more info	PubMed
involved_in protein sumoylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein sumoylation	IDA Inferred from Direct Assay more info	PubMed
involved_in protein sumoylation	IEA Inferred from Electronic Annotation more info
involved_in protein sumoylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein sumoylation	TAS Traceable Author Statement more info
involved_in ubiquitin-dependent protein catabolic process	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
located_in PML body	IDA Inferred from Direct Assay more info	PubMed
part_of SUMO ligase complex	IPI Inferred from Physical Interaction more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	TAS Traceable Author Statement more info
located_in nuclear envelope	TAS Traceable Author Statement more info
part_of nuclear pore	NAS Non-traceable Author Statement more info	PubMed
located_in nucleoplasm	TAS Traceable Author Statement more info
located_in nucleus	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in perinuclear region of cytoplasm	IEA Inferred from Electronic Annotation more info
located_in synaptonemal complex	TAS Traceable Author Statement more info	PubMed
part_of transferase complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: SUMO-conjugating enzyme UBC9

Names: RING-type E3 SUMO transferase UBC9; SUMO-1-protein ligase; SUMO-protein ligase; ubiquitin carrier protein 9; ubiquitin carrier protein I; ubiquitin conjugating enzyme 9; ubiquitin conjugating enzyme E2I; ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast); ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9); ubiquitin-conjugating enzyme UbcE2A; ubiquitin-like protein SUMO-1 conjugating enzyme; ubiquitin-protein ligase E2I; ubiquitin-protein ligase I

NP_003336.1

EC 6.3.2.19

NP_919235.1

EC 6.3.2.19

NP_919236.1

EC 6.3.2.19

NP_919237.1

EC 6.3.2.19

XP_005255597.1

EC 6.3.2.19

XP_011520946.1

EC 6.3.2.19

XP_011520947.1

EC 6.3.2.19

XP_047290540.1

EC 6.3.2.19

XP_047290541.1

EC 6.3.2.19

XP_054169802.1

EC 6.3.2.19

XP_054169803.1

EC 6.3.2.19

XP_054169804.1

EC 6.3.2.19

XP_054169805.1

EC 6.3.2.19

XP_054169806.1

EC 6.3.2.19

XP_054169807.1

EC 6.3.2.19

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_003345.5 → NP_003336.1 SUMO-conjugating enzyme UBC9

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the predominant transcript. Variants 1-4 encode the same protein.

Source sequence(s)

AK291118, AL031714, BC000427, BI793063

Consensus CDS

CCDS10433.1

UniProtKB/Swiss-Prot

D3DU69, P50550, P63279, Q15698, Q59GX1, Q86VB3

UniProtKB/TrEMBL

A8K503

Related

ENSP00000380649.3, ENST00000397514.8

Conserved Domains (1) summary

pfam00179
Location:8 → 152

UQ_con; Ubiquitin-conjugating enzyme
NM_194259.3 → NP_919235.1 SUMO-conjugating enzyme UBC9

Status: REVIEWED

Description

Transcript Variant: This variant (2) has an additional exon in the 5' UTR, as compared to variant 1.

Source sequence(s)

AK291118, AL031714, BF315618, BI793063, D45050

Consensus CDS

CCDS10433.1

UniProtKB/Swiss-Prot

D3DU69, P50550, P63279, Q15698, Q59GX1, Q86VB3

UniProtKB/TrEMBL

A8K503

Related

ENSP00000380650.2, ENST00000397515.7

Conserved Domains (1) summary

pfam00179
Location:8 → 152

UQ_con; Ubiquitin-conjugating enzyme
NM_194260.3 → NP_919236.1 SUMO-conjugating enzyme UBC9

Status: REVIEWED

Description

Transcript Variant: This variant (3) differs in the 5' UTR, as compared to variant 1.

Source sequence(s)

AC120498, AL031714, BI793063, CX788607

Consensus CDS

CCDS10433.1

UniProtKB/Swiss-Prot

D3DU69, P50550, P63279, Q15698, Q59GX1, Q86VB3

UniProtKB/TrEMBL

A8K503

Related

ENSP00000348056.4, ENST00000355803.9

Conserved Domains (1) summary

pfam00179
Location:8 → 152

UQ_con; Ubiquitin-conjugating enzyme
NM_194261.3 → NP_919237.1 SUMO-conjugating enzyme UBC9

Status: REVIEWED

Description

Transcript Variant: This variant (4) differs in the 5' UTR, as compared to variant 1.

Source sequence(s)

AB208988, AC120498, AL031714, BI793063

Consensus CDS

CCDS10433.1

UniProtKB/Swiss-Prot

D3DU69, P50550, P63279, Q15698, Q59GX1, Q86VB3

UniProtKB/TrEMBL

A8K503

Related

ENSP00000324897.5, ENST00000325437.10

Conserved Domains (1) summary

pfam00179
Location:8 → 152

UQ_con; Ubiquitin-conjugating enzyme

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

NC_000016.10 Reference GRCh38.p14 Primary Assembly

Range

1309152..1327017

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_047434585.1 → XP_047290541.1 SUMO-conjugating enzyme UBC9 isoform X1
XM_011522645.2 → XP_011520947.1 SUMO-conjugating enzyme UBC9 isoform X1

Conserved Domains (2) summary

COG5078
Location:1 → 137

COG5078; Ubiquitin-protein ligase [Posttranslational modification, protein turnover, chaperones]

pfam00179
Location:8 → 137

UQ_con; Ubiquitin-conjugating enzyme
XM_047434584.1 → XP_047290540.1 SUMO-conjugating enzyme UBC9 isoform X1
XM_011522644.3 → XP_011520946.1 SUMO-conjugating enzyme UBC9 isoform X1

Conserved Domains (2) summary

COG5078
Location:1 → 137

COG5078; Ubiquitin-protein ligase [Posttranslational modification, protein turnover, chaperones]

pfam00179
Location:8 → 137

UQ_con; Ubiquitin-conjugating enzyme
XM_005255540.3 → XP_005255597.1 SUMO-conjugating enzyme UBC9 isoform X1

Conserved Domains (2) summary

COG5078
Location:1 → 137

COG5078; Ubiquitin-protein ligase [Posttranslational modification, protein turnover, chaperones]

pfam00179
Location:8 → 137

UQ_con; Ubiquitin-conjugating enzyme

Alternate T2T-CHM13v2.0

Genomic

NC_060940.1 Alternate T2T-CHM13v2.0

Range

1325332..1343194

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_054313832.1 → XP_054169807.1 SUMO-conjugating enzyme UBC9 isoform X1

UniProtKB/TrEMBL

Q7KZS0
XM_054313831.1 → XP_054169806.1 SUMO-conjugating enzyme UBC9 isoform X1

UniProtKB/TrEMBL

Q7KZS0
XM_054313827.1 → XP_054169802.1 SUMO-conjugating enzyme UBC9 isoform X1

UniProtKB/TrEMBL

Q7KZS0
XM_054313830.1 → XP_054169805.1 SUMO-conjugating enzyme UBC9 isoform X1

UniProtKB/TrEMBL

Q7KZS0
XM_054313828.1 → XP_054169803.1 SUMO-conjugating enzyme UBC9 isoform X1

UniProtKB/TrEMBL

Q7KZS0
XM_054313829.1 → XP_054169804.1 SUMO-conjugating enzyme UBC9 isoform X1

UniProtKB/TrEMBL

Q7KZS0