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    TOP2B DNA topoisomerase II beta [ Homo sapiens (human) ]

    Gene ID: 7155, updated on 28-Oct-2024

    Summary

    Official Symbol
    TOP2Bprovided by HGNC
    Official Full Name
    DNA topoisomerase II betaprovided by HGNC
    Primary source
    HGNC:HGNC:11990
    See related
    Ensembl:ENSG00000077097 MIM:126431; AllianceGenome:HGNC:11990
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    BILU; TOPIIB; top2beta
    Summary
    This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016]
    Expression
    Ubiquitous expression in urinary bladder (RPKM 32.3), brain (RPKM 31.7) and 25 other tissues See more
    Orthologs
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    See TOP2B in Genome Data Viewer
    Location:
    3p24.2
    Exon count:
    36
    Annotation release Status Assembly Chr Location
    RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 3 NC_000003.12 (25597905..25664907, complement)
    RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 3 NC_060927.1 (25600752..25667754, complement)
    RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 3 NC_000003.11 (25639396..25706398, complement)

    Chromosome 3 - NC_000003.12Genomic Context describing neighboring genes Neighboring gene retinoic acid receptor beta Neighboring gene H3K4me1 hESC enhancer GRCh37_chr3:25491906-25492406 Neighboring gene NANOG-H3K27ac hESC enhancer GRCh37_chr3:25495987-25496611 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr3:25496710-25497250 Neighboring gene uncharacterized LOC124909356 Neighboring gene H3 histone pseudogene 10 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr3:25609805-25610306 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr3:25614420-25614948 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr3:25614949-25615476 Neighboring gene NUP98-TOP2B recombination region Neighboring gene ATAC-STARR-seq lymphoblastoid active region 19608 Neighboring gene ReSE screen-validated silencer GRCh37_chr3:25705465-25705653 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 14146 Neighboring gene H3K27ac hESC enhancer GRCh37_chr3:25706135-25706698 Neighboring gene microRNA 4442 Neighboring gene cysteine rich protein 1 pseudogene 2 Neighboring gene ribosomal protein L32 pseudogene 11 Neighboring gene N-glycanase 1

    Genomic regions, transcripts, and products

    Expression

    • Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?

    HIV-1 interactions

    Replication interactions

    Interaction Pubs
    HIV-1 replication is enhanced by over-expression of TOP2B (TopoisomeraseIIb) PubMed
    HIV-1 replication requires TOP2B (TopoisomeraseIIb) as shown through inhibition by ICRF-193 or merbarone treatment or siRNA knockdown PubMed
    Knockdown of TOP2A, TOP2B, or TOP2A/2B by siRNA significantly decreases HIV-1 replication in Sup T1 cells PubMed

    Protein interactions

    Protein Gene Interaction Pubs
    Tat tat HIV-1 Tat binds TOP2B (topoisomeraseIIb), XRCC6 (Ku70), and PARP1 during transactivation and viral replication PubMed
    capsid gag Knockdown of TOP2A, TOP2B, or TOP2A/2B by siRNA significantly decreases p24 levels in HIV-1 infected cell culture supernatants when compared to control PubMed
    reverse transcriptase gag-pol HIV-1 RT co-localizes with TOP2A and TOP2B in cytosol of HIV-1 infected Sup T1 and PBMC cells PubMed

    Go to the HIV-1, Human Interaction Database

    Pathways from PubChem

    Interactions

    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Markers

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
     
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
     
    enables DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    enables chromatin binding IDA
    Inferred from Direct Assay
    more info
    PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
     
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    enables ribonucleoprotein complex binding IEA
    Inferred from Electronic Annotation
    more info
     
    Process Evidence Code Pubs
    involved_in B cell differentiation IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in DNA topological change IDA
    Inferred from Direct Assay
    more info
    PubMed 
    involved_in axonogenesis IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in cellular response to ATP IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in cellular response to hydrogen peroxide IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in cellular senescence IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in forebrain development IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in neuron migration IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in positive regulation of double-strand break repair via nonhomologous end joining IEA
    Inferred from Electronic Annotation
    more info
     
    involved_in positive regulation of single stranded viral RNA replication via double stranded DNA intermediate IMP
    Inferred from Mutant Phenotype
    more info
    PubMed 
    involved_in resolution of meiotic recombination intermediates IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    involved_in sister chromatid segregation IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    Component Evidence Code Pubs
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    PubMed 
    colocalizes_with heterochromatin IDA
    Inferred from Direct Assay
    more info
    PubMed 
    NOT located_in nucleolus IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in nucleolus IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in nucleoplasm IDA
    Inferred from Direct Assay
    more info
    PubMed 
    located_in nucleoplasm TAS
    Traceable Author Statement
    more info
     
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    PubMed 
    part_of ribonucleoprotein complex ISS
    Inferred from Sequence or Structural Similarity
    more info
     

    General protein information

    Preferred Names
    DNA topoisomerase 2-beta
    Names
    DNA topoisomerase II, 180 kD
    DNA topoisomerase II, beta isozyme
    U937 associated antigen
    antigen MLAA-44
    topo II beta
    topoisomerase (DNA) II beta 180kDa
    topoisomerase II beta
    topoisomerase IIb
    NP_001059.2
    NP_001317629.1
    XP_011532359.1
    XP_047304777.1
    XP_047304778.1
    XP_054203683.1
    XP_054203684.1
    XP_054203685.1

    NCBI Reference Sequences (RefSeq)

    NEW Try the new Transcript table

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    Genomic

    1. NG_052961.1 RefSeqGene

      Range
      4466..71389
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001068.3NP_001059.2  DNA topoisomerase 2-beta isoform 2

      See identical proteins and their annotated locations for NP_001059.2

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2) contains an alternate in-frame splice site in the 5' coding region, compared to variant 1. It encodes isoform 2, which is shorter than isoform 1.
      Source sequence(s)
      AB208879, BF514624, BM150930, X68060
      Consensus CDS
      CCDS46776.1
      UniProtKB/TrEMBL
      Q71UH4
      Related
      ENSP00000396704.2, ENST00000435706.6
      Conserved Domains (7) summary
      cd03365
      Location:471591
      TOPRIM_TopoIIA; TOPRIM_TopoIIA: topoisomerase-primase (TOPRIM) nucleotidyl transferase/hydrolase domain of the type found in proteins of the type IIA family of DNA topoisomerases similar to Saccharomyces cerevisiae Topoisomerase II. TopoIIA enzymes cut both strands of ...
      cd00187
      Location:7271187
      TOP4c; DNA Topoisomerase, subtype IIA; domain A'; bacterial DNA topoisomerase IV (C subunit, ParC), bacterial DNA gyrases (A subunit, GyrA),mammalian DNA toposiomerases II. DNA topoisomerases are essential enzymes that regulate the conformational changes in DNA ...
      cd03481
      Location:281433
      TopoIIA_Trans_ScTopoIIA; TopoIIA_Trans_ScTopoIIA: Transducer domain, having a ribosomal S5 domain 2-like fold, of the type found in proteins of the type IIA family of DNA topoisomerases similar to Saccharomyces cerevisiae Topo IIA. S. cerevisiae Topo IIA is a homodimer encoded ...
      PTZ00108
      Location:411466
      PTZ00108; DNA topoisomerase 2-like protein; Provisional
      pfam02518
      Location:96193
      HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
      pfam08070
      Location:15031604
      DTHCT; DTHCT (NUC029) region
      pfam16898
      Location:589727
      TOPRIM_C; C-terminal associated domain of TOPRIM
    2. NM_001330700.2NP_001317629.1  DNA topoisomerase 2-beta isoform 1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
      Source sequence(s)
      AC093416
      Consensus CDS
      CCDS82746.1
      UniProtKB/Swiss-Prot
      Q02880, Q13600, Q9UMG8, Q9UQP8
      UniProtKB/TrEMBL
      Q71UH4
      Related
      ENSP00000264331.4, ENST00000264331.9

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000003.12 Reference GRCh38.p14 Primary Assembly

      Range
      25597905..25664907 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_047448821.1XP_047304777.1  DNA topoisomerase 2-beta isoform X2

      Related
      ENSP00000514098.1, ENST00000699043.1
    2. XM_011534057.4XP_011532359.1  DNA topoisomerase 2-beta isoform X1

      UniProtKB/TrEMBL
      Q71UH4
      Conserved Domains (7) summary
      cd03365
      Location:476596
      TOPRIM_TopoIIA; TOPRIM_TopoIIA: topoisomerase-primase (TOPRIM) nucleotidyl transferase/hydrolase domain of the type found in proteins of the type IIA family of DNA topoisomerases similar to Saccharomyces cerevisiae Topoisomerase II. TopoIIA enzymes cut both strands of ...
      cd00187
      Location:7321192
      TOP4c; DNA Topoisomerase, subtype IIA; domain A'; bacterial DNA topoisomerase IV (C subunit, ParC), bacterial DNA gyrases (A subunit, GyrA),mammalian DNA toposiomerases II. DNA topoisomerases are essential enzymes that regulate the conformational changes in DNA ...
      cd03481
      Location:286438
      TopoIIA_Trans_ScTopoIIA; TopoIIA_Trans_ScTopoIIA: Transducer domain, having a ribosomal S5 domain 2-like fold, of the type found in proteins of the type IIA family of DNA topoisomerases similar to Saccharomyces cerevisiae Topo IIA. S. cerevisiae Topo IIA is a homodimer encoded ...
      PTZ00108
      Location:461466
      PTZ00108; DNA topoisomerase 2-like protein; Provisional
      pfam02518
      Location:101198
      HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
      pfam08070
      Location:14711572
      DTHCT; DTHCT (NUC029) region
      pfam16898
      Location:594732
      TOPRIM_C; C-terminal associated domain of TOPRIM
    3. XM_047448822.1XP_047304778.1  DNA topoisomerase 2-beta isoform X3

      Related
      ENSP00000391112.1, ENST00000424225.1

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060927.1 Alternate T2T-CHM13v2.0

      Range
      25600752..25667754 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_054347709.1XP_054203684.1  DNA topoisomerase 2-beta isoform X2

    2. XM_054347708.1XP_054203683.1  DNA topoisomerase 2-beta isoform X1

    3. XM_054347710.1XP_054203685.1  DNA topoisomerase 2-beta isoform X3