MPH1 3'-5' DNA helicase [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: MPH1
Official Full Name: 3'-5' DNA helicase
Primary source: SGD:S000001441
Locus tag: YIR002C
See related: AllianceGenome:SGD:S000001441; FungiDB:YIR002C; VEuPathDB:YIR002C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables 3'-5' DNA helicase activity; DNA/RNA helicase activity; and flap-structured DNA binding activity. Involved in DNA metabolic process; donor selection; and negative regulation of strand invasion. Located in nucleus. Used to study Fanconi anemia. Human ortholog(s) of this gene implicated in primary ovarian insufficiency 15 and spermatogenic failure 28. Orthologous to human FANCM (FA complementation group M). [provided by Alliance of Genome Resources, Oct 2024]
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Genomic context

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See MPH1 in Genome Data Viewer

Location:: chromosome: IX

Exon count:: 1

Sequence:: Chromosome: IX; NC_001141.2 (357415..360396, complement)

Chromosome IX - NC_001141.2 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001141.2

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Interstitial telomere sequences disrupt break-induced replication and drive formation of ectopic telomeres.
Title: Interstitial telomere sequences disrupt break-induced replication and drive formation of ectopic telomeres.
Mph1 helicase localizes to the mitochondria and contributes to mtDNA stability
Title: The FANCM family Mph1 helicase localizes to the mitochondria and contributes to mtDNA stability.
Through molecular genetic analysis of this defined fork-stalling event, coupled with the use of a genome-wide genetic screen, we identified an important role for the SUMO-like domain protein, Esc2, in limiting genome rearrangements at a telomere. Moreover, we showed that these rearrangements are driven by the combined action of the Mph1 helicase and the homologous recombination machinery
Title: Esc2 promotes telomere stability in response to DNA replication stress.
Mph1 is a double-edged sword whose action at hybrids must be regulated by the Smc5/6 complex. This is underlined by the observation that simultaneous inactivation of RNase H2 and Smc5/6 results in Mph1-dependent synthetic lethality.
Title: The Smc5/6 complex regulates the yeast Mph1 helicase at RNA-DNA hybrid-mediated DNA damage.
mms21RINGDelta mutant exhibits (1) reduced ribosomal RNA production; (2) nuclear accumulation of ribosomal proteins; (3) elevated Gcn4 translation, indicating translational stress; We identified RPL19A as a novel genetic suppressor of the mms21RINGDelta mutant; Deletion of a previously identified strong suppressor, MPH1, rescues both the accumulation of ribosome subunits and translational stress
Title: Mms21 SUMO Ligase Activity Promotes Nucleolar Function in Saccharomyces cerevisiae.
Mutator phenotype of MPH1 overexpression suggests a novel gain-of-function mechanism. DEAH-box function was required for hypermutation, mutagenesis by MPH1 overexpression was independent of translesion synthesis, but was suppressed by overexpression of RAD27.
Title: Dosage Mutator Genes in Saccharomyces cerevisiae: A Novel Mutator Mode-of-Action of the Mph1 DNA Helicase.
Mph1regulates the REC-mediated choice between gene conversion and break-induced replication (BIR). Double deletion of Sgs1 and Mph1 abolishes the REC-mediated lag observed during the repair of large gaps and BIR substrates.
Title: Sgs1 and Mph1 Helicases Enforce the Recombination Execution Checkpoint During DNA Double-Strand Break Repair in Saccharomyces cerevisiae.
Mph1 and Mte1 interdependently colocalize at DNA damage-induced foci and dysfunctional telomeres, and MTE1 deletion results in elongated telomeres
Title: Mte1 interacts with Mph1 and promotes crossover recombination and telomere maintenance.
Mte1 functions with Mph1 and the associated MHF complex in replication fork repair
Title: Differential regulation of the anti-crossover and replication fork regression activities of Mph1 by Mte1.
Data support the hypothesis that Mph1 is the main helicase responsible for template switching during break-induced replication.
Title: Template switching during break-induced replication is promoted by the Mph1 helicase in Saccharomyces cerevisiae.

Submit: New GeneRIF Correction See all GeneRIFs (22)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 3'-5' DNA helicase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 3'-5' DNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
enables 3'-5' DNA helicase activity	IEA Inferred from Electronic Annotation more info
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA/RNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
enables flap-structured DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables four-way junction DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables four-way junction helicase activity	IBA Inferred from Biological aspect of Ancestor more info
enables helicase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA duplex unwinding	IEA Inferred from Electronic Annotation more info
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in DNA replication, Okazaki fragment processing	IGI Inferred from Genetic Interaction more info	PubMed
involved_in donor selection	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in donor selection	IPI Inferred from Physical Interaction more info	PubMed
involved_in double-strand break repair via synthesis-dependent strand annealing	IBA Inferred from Biological aspect of Ancestor more info
involved_in interstrand cross-link repair	IBA Inferred from Biological aspect of Ancestor more info
involved_in interstrand cross-link repair	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of strand invasion	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of strand invasion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in recombinational repair	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: 3'-5' DNA helicase

NP_012267.1

EC 3.6.4.12

3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds flap DNA, stimulates activity of Rad27p and Dna2p; prevents crossovers between ectopic sequences by removing substrates for Mus81-Mms4 or Rad1-Rad10 cleavage; homolog of human FANCM Fanconi anemia protein that is involved in stabilizing and remodeling blocked replication forks; member of SF2 DExD/H superfamily of helicases; nonsense or missense mutations in FANCM predispose individuals to breast cancer

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001141.2 Reference assembly

Range

357415..360396 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179524.1 → NP_012267.1 TPA: 3'-5' DNA helicase [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012267.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VVT2, P40562

UniProtKB/TrEMBL

B3LTK7, C7GJH0, C8ZAQ1, N1P973

Conserved Domains (1) summary

COG1111
Location:72 → 675

MPH1; ERCC4-related helicase [Replication, recombination and repair]