Slc32a1 solute carrier family 32 member 1 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Slc32a1provided by RGD
Official Full Name: solute carrier family 32 member 1provided by RGD
Primary source: RGD:621402
See related: EnsemblRapid:ENSRNOG00000015393 AllianceGenome:RGD:621402
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Vgat; Viaat; RUNC-47
Summary: Enables gamma-aminobutyric acid:proton antiporter activity and glycine transmembrane transporter activity. Involved in carboxylic acid transport; hippocampus development; and neurotransmitter loading into synaptic vesicle. Located in inhibitory synapse and synaptic vesicle. Is active in GABA-ergic synapse and synaptic vesicle membrane. Human ortholog(s) of this gene implicated in developmental and epileptic encephalopathy and generalized epilepsy with febrile seizures plus. Orthologous to human SLC32A1 (solute carrier family 32 member 1). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Restricted expression toward (RPKM 98.9) See more
Orthologs: human mouse all
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Genomic context

Location:: 3q42

Exon count:: 2

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	3	NC_086021.1 (167687245..167691714)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	3	NC_051338.1 (147267373..147271842)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	3	NC_005102.4 (155102980..155107815)

Chromosome 3 - NC_086021.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

cortical axon terminals co-expressing VGLUT1 and VGAT can evoke simultaneously monosynaptic glutamate and gamma-aminobutyric acid responses.
Title: Co-expression of VGLUT1 and VGAT sustains glutamate and GABA co-release and is regulated by activity in cortical neurons.
Data show that VIAAT is a vesicular Cl(-) transporter that co-transports Cl(-) with GABA or glycine in a membrane-potential-dependent manner, and that Cl(-) plays an essential role in vesicular storage of GABA and glycine.
Title: Vesicular inhibitory amino acid transporter is a Cl-/gamma-aminobutyrate Co-transporter.
GABAergic synapses in cortical axon terminals express both vesicular transporters VGAT and VGLUT1.
Title: VGLUT1 and VGAT are sorted to the same population of synaptic vesicles in subsets of cortical axon terminals.
VGLUT1 is sorted diurnally to the plasma membrane to modulate glutamate transmission during a day/night cycle, whereas VGAT expression is not oscillating but is increased in the presence of a light/dark cycle.
Title: Time of day-dependent sorting of the vesicular glutamate transporter to the plasma membrane.
Concomitantly with the loss of some subpopulations of VGAT-containing neurons, there was an up-regulation of VGAT synthesis in all remaining GABA neurons as early as 1 week after pilocarpine injection.
Title: Changes in vesicular transporters for gamma-aminobutyric acid and glutamate reveal vulnerability and reorganization of hippocampal neurons following pilocarpine-induced seizures.
In mature neocortex neuronal cultures, VGLUT1 and VIAAT exhibit bidirectional and opposite regulation by prolonged activity changes.
Title: Homeostatic scaling of vesicular glutamate and GABA transporter expression in rat neocortical circuits.
functional neuronal VIAAT is present in glucagon-containing secretory granules in pancreatic alpha-cells
Title: Vesicular inhibitory amino acid transporter is present in glucagon-containing secretory granules in alphaTC6 cells, mouse clonal alpha-cells, and alpha-cells of islets of Langerhans.
Glycine is released from synaptic vesicles when VIAAT is coexpressed with the neural transporter GlyT2.
Title: The transporters GlyT2 and VIAAT cooperate to determine the vesicular glycinergic phenotype.
As a whole, the results provide original evidence that vGAT expression is altered in the 6-hydroxydopamine model of Parkinson's disease.
Title: Unilateral 6-hydroxydopamine lesion of dopamine neurons and subchronic L-DOPA administration in the adult rat alters the expression of the vesicular GABA transporter in different subsets of striatal neurons and in the substantia nigra, pars reticulata.
VIAAT is expressed in rat islets and is more abundant in the mantle
Title: Expression of the vesicular inhibitory amino acid transporter in pancreatic islet cells: distribution of the transporter within rat islets.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Slc32a1 (e-PCR), detects polymorphism
- UniSTS:507228

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables gamma-aminobutyric acid transmembrane transporter activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables gamma-aminobutyric acid:proton antiporter activity	IDA Inferred from Direct Assay more info	PubMed
enables gamma-aminobutyric acid:proton antiporter activity	ISO Inferred from Sequence Orthology more info
enables glycine transmembrane transporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables glycine transmembrane transporter activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables glycine transmembrane transporter activity	ISO Inferred from Sequence Orthology more info
enables glycine transmembrane transporter activity	TAS Traceable Author Statement more info	PubMed
enables glycine:proton antiporter activity	ISO Inferred from Sequence Orthology more info
enables glycine:proton antiporter activity	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
involved_in beta-alanine transport	IDA Inferred from Direct Assay more info	PubMed
involved_in gamma-aminobutyric acid import	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in gamma-aminobutyric acid import	ISO Inferred from Sequence Orthology more info
involved_in gamma-aminobutyric acid import	ISS Inferred from Sequence or Structural Similarity more info
involved_in gamma-aminobutyric acid transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in gamma-aminobutyric acid transport	IDA Inferred from Direct Assay more info	PubMed
involved_in gamma-aminobutyric acid transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in glycine transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in glycine transport	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within glycine transport	ISO Inferred from Sequence Orthology more info
involved_in glycine transport	ISO Inferred from Sequence Orthology more info
involved_in glycine transport	ISS Inferred from Sequence or Structural Similarity more info
involved_in glycine transport	TAS Traceable Author Statement more info	PubMed
involved_in hippocampus development	IEP Inferred from Expression Pattern more info	PubMed
involved_in neurotransmitter loading into synaptic vesicle	IBA Inferred from Biological aspect of Ancestor more info
involved_in neurotransmitter loading into synaptic vesicle	IDA Inferred from Direct Assay more info	PubMed
involved_in neurotransmitter loading into synaptic vesicle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neurotransmitter transport	TAS Traceable Author Statement more info	PubMed
involved_in proton transmembrane transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in GABA-ergic synapse	IDA Inferred from Direct Assay more info	PubMed
located_in cell surface	ISO Inferred from Sequence Orthology more info
located_in cell tip	ISO Inferred from Sequence Orthology more info
located_in cone cell pedicle	ISO Inferred from Sequence Orthology more info
located_in dendrite	ISO Inferred from Sequence Orthology more info
is_active_in dendrite terminus	IBA Inferred from Biological aspect of Ancestor more info
located_in dendrite terminus	ISO Inferred from Sequence Orthology more info
located_in inhibitory synapse	IDA Inferred from Direct Assay more info	PubMed
located_in inhibitory synapse	ISO Inferred from Sequence Orthology more info
located_in neuron projection	ISO Inferred from Sequence Orthology more info
is_active_in neuron projection terminus	IBA Inferred from Biological aspect of Ancestor more info
located_in neuron projection terminus	ISO Inferred from Sequence Orthology more info
located_in presynapse	IDA Inferred from Direct Assay more info	PubMed
located_in presynaptic active zone	ISO Inferred from Sequence Orthology more info
located_in synapse	IDA Inferred from Direct Assay more info	PubMed
located_in synaptic vesicle	IDA Inferred from Direct Assay more info	PubMed
is_active_in synaptic vesicle membrane	IBA Inferred from Biological aspect of Ancestor more info
is_active_in synaptic vesicle membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: vesicular inhibitory amino acid transporter

Names: GABA and glycine transporter; UNC-47 homolog; rGVAT; solute carrier family 32 (GABA vesicular transporter), member 1; vesicular GABA transporter; vesicular inhibitory amino acid transporter-like

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.