RPL3 ribosomal protein L3 [Homo sapiens (human)] - Gene

Summary

Official Symbol: RPL3provided by HGNC
Official Full Name: ribosomal protein L3provided by HGNC
Primary source: HGNC:HGNC:10332
See related: Ensembl:ENSG00000100316 MIM:604163; AllianceGenome:HGNC:10332
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: L3; uL3; ASC-1; TARBP-B
Summary: Ribosomes, the complexes that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L3P family of ribosomal proteins and it is located in the cytoplasm. The protein can bind to the HIV-1 TAR mRNA, and it has been suggested that the protein contributes to tat-mediated transactivation. This gene is co-transcribed with several small nucleolar RNA genes, which are located in several of this gene's introns. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
Expression: Ubiquitous expression in ovary (RPKM 2560.0), skin (RPKM 1107.2) and 25 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 22q13.1

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCh38.p14 (GCF_000001405.40)	22	NC_000022.11 (39312882..39319623, complement)
RS_2024_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	22	NC_060946.1 (39783532..39790275, complement)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	22	NC_000022.10 (39708887..39715628, complement)

Chromosome 22 - NC_000022.11 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

BAIAP2L1 accelerates breast cancer progression and chemoresistance by activating AKT signaling through binding with ribosomal protein L3.
Title: BAIAP2L1 accelerates breast cancer progression and chemoresistance by activating AKT signaling through binding with ribosomal protein L3.
Suppression of RPL34 Inhibits Tumor Cell Proliferation and Promotes Apoptosis in Glioblastoma.
Title: Suppression of RPL34 Inhibits Tumor Cell Proliferation and Promotes Apoptosis in Glioblastoma.
METTL18-mediated histidine methylation of RPL3 modulates translation elongation for proteostasis maintenance.
Title: METTL18-mediated histidine methylation of RPL3 modulates translation elongation for proteostasis maintenance.
S-Adenosyl-l-Methionine Overcomes uL3-Mediated Drug Resistance in p53 Deleted Colon Cancer Cells.
Title: S-Adenosyl-l-Methionine Overcomes uL3-Mediated Drug Resistance in p53 Deleted Colon Cancer Cells.
DUOX2 promotes the progression of colorectal cancer cells by regulating the AKT pathway and interacting with RPL3.
Title: DUOX2 promotes the progression of colorectal cancer cells by regulating the AKT pathway and interacting with RPL3.
Human METTL18 is a histidine-specific methyltransferase that targets RPL3 and affects ribosome biogenesis and function.
Title: Human METTL18 is a histidine-specific methyltransferase that targets RPL3 and affects ribosome biogenesis and function.
uL3 Mediated Nucleolar Stress Pathway as a New Mechanism of Action of Antiproliferative G-quadruplex TBA Derivatives in Colon Cancer Cells.
Title: uL3 Mediated Nucleolar Stress Pathway as a New Mechanism of Action of Antiproliferative G-quadruplex TBA Derivatives in Colon Cancer Cells.
Ribosomal protein uL3 targets E2F1 and Cyclin D1 in cancer cell response to nucleolar stress.
Title: Ribosomal protein uL3 targets E2F1 and Cyclin D1 in cancer cell response to nucleolar stress.
The sensitivity of p53 null colon cancer cells to 5-FU can be enhanced using proapoptotic rpL3 gene.
Title: Enhancement of 5-FU sensitivity by the proapoptotic rpL3 gene in p53 null colon cancer cells through combined polymer nanoparticles.
nucleolar stress induced by 5-fluorouracil in colon cancer cells devoid of p53 leads to the activation of ribosomal protein L3 as proapoptotic factor
Title: 5-FU targets rpL3 to induce mitochondrial apoptosis via cystathionine-β-synthase in colon cancer cells lacking p53.

Submit: New GeneRIF Correction See all GeneRIFs (16)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Replication interactions

Interaction	Pubs
Knockdown of ribosomal protein L3 (RPL3) by shRNA library screening inhibits HIV-1 replication in cultured Jurkat T-cells	PubMed

Protein interactions

Protein	Gene	Interaction	Pubs
Envelope surface glycoprotein gp120	env	Tandem affinity purification and mass spectrometry analysis identify ribosomal protein L3 (RPL3; HIV-1 TAR RNA-binding protein B), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells	PubMed
Gag-Pol	gag-pol	Tandem affinity purification and mass spectrometry analysis identify ribosomal protein L3 (RPL3; HIV-1 TAR RNA-binding protein B), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells	PubMed
Nef	nef	Tandem affinity purification and mass spectrometry analysis identify ribosomal protein L3 (RPL3; HIV-1 TAR RNA-binding protein B), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells	PubMed
Pr55(Gag)	gag	Tandem affinity purification and mass spectrometry analysis identify ribosomal protein L3 (RPL3; HIV-1 TAR RNA-binding protein B), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells	PubMed
Rev	rev	HIV-1 Rev interacting protein, ribosomal protein L3 (RPL3), is identified by the in-vitro binding experiments involving cytosolic or nuclear extracts from HeLa cells	PubMed
Tat	tat	TARBP-b binds to HIV-1 TAR RNA and data suggests that TARBP-b interacts with HIV-1 Tat to mediate transactivation of the HIV-1 LTR promoter	PubMed
retropepsin	gag-pol	Positional proteomics analysis identifies the cleavage of human 60S ribosomal protein L3 (RPL3) at amino acid residues 331-332 and 381-382 by the HIV-1 protease	PubMed

Go to the HIV-1, Human Interaction Database

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RP_L3_1 (e-PCR)
- UniSTS:52743

RH66576 (e-PCR)
- UniSTS:90143

D8S2279 (e-PCR)
- UniSTS:473907

MARC_6327-6328:996689678:1 (e-PCR)
- UniSTS:269637

D22S1257 (e-PCR)
- UniSTS:29543

G65385 (e-PCR)
- UniSTS:166517

G65386 (e-PCR)
- UniSTS:166518

G65387 (e-PCR)
- UniSTS:166519

D17S1982 (e-PCR)
- UniSTS:32378

D14S1219 (e-PCR)
- UniSTS:47498

D11S3114 (e-PCR)
- UniSTS:152207

D17S891E (e-PCR)
- UniSTS:473040

SGC33142 (e-PCR)
- UniSTS:52725

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables RNA binding	HDA more info	PubMed
enables RNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables structural constituent of ribosome	IBA Inferred from Biological aspect of Ancestor more info
enables structural constituent of ribosome	IDA Inferred from Direct Assay more info	PubMed
enables structural constituent of ribosome	NAS Non-traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
involved_in cellular response to interleukin-4	IEA Inferred from Electronic Annotation more info
involved_in cytoplasmic translation	IC Inferred by Curator more info	PubMed
involved_in translation	IBA Inferred from Biological aspect of Ancestor more info
involved_in translation	NAS Non-traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info
located_in cytosol	TAS Traceable Author Statement more info
part_of cytosolic large ribosomal subunit	HDA more info	PubMed
part_of cytosolic large ribosomal subunit	IBA Inferred from Biological aspect of Ancestor more info
part_of cytosolic large ribosomal subunit	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytosolic ribosome	IDA Inferred from Direct Assay more info	PubMed
located_in cytosolic ribosome	IDA Inferred from Direct Assay more info	PubMed
located_in extracellular exosome	HDA more info	PubMed
located_in focal adhesion	HDA more info	PubMed
located_in nucleolus	HDA more info	PubMed
located_in nucleolus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	HDA more info	PubMed
part_of protein-containing complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: large ribosomal subunit protein uL3

Names: 60S ribosomal protein L3; HIV-1 TAR RNA-binding protein B

NP_000958.1

EC 3.6.5.3

NP_001029025.1

EC 3.6.5.3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_000967.4 → NP_000958.1 large ribosomal subunit protein uL3 isoform a

See identical proteins and their annotated locations for NP_000958.1

Status: REVIEWED

Description

Transcript Variant: This variant (1) encodes the longest isoform (a).

Source sequence(s)

AK130450, BC012146, BC071666

Consensus CDS

CCDS13988.1

UniProtKB/Swiss-Prot

B2RDV9, P39023, Q15548, Q5I0G0

UniProtKB/TrEMBL

Q96QL0

Related

ENSP00000346001.3, ENST00000216146.9

Conserved Domains (1) summary

PTZ00103
Location:1 → 400

PTZ00103; 60S ribosomal protein L3; Provisional
NM_001033853.2 → NP_001029025.1 large ribosomal subunit protein uL3 isoform b

See identical proteins and their annotated locations for NP_001029025.1

Status: REVIEWED

Description

Transcript Variant: This variant (2) uses alternate in-frame splice sites in two exons, compared to variant 1, resulting in a shorter protein (isoform b).

Source sequence(s)

AK092695, BC008492, BC071666

UniProtKB/TrEMBL

B3KS36

Conserved Domains (1) summary

cl00324
Location:1 → 351

Ribosomal_L3; Ribosomal protein L3