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Platform GPL10227 Query DataSets for GPL10227
Status Public on Mar 19, 2010
Title ARK-Genomics G. gallus 20K v2 (single gene version)
Technology type spotted oligonucleotide
Distribution non-commercial
Organism Gallus gallus
Manufacturer ARK-Genomics, University of Edinburgh, UK
Manufacture protocol Oligos were resuspended in Corning Pronto Epoxide spotting solution to give a final concentration of 20uM. DNA was printed on Corning Epoxide slides using a Genomic Solutions MicroGrid II arrayer and the MicroSpot 10K pins, according to manufacturer's instructions. All printing was carried out in conditions of constant humidity (52% RH) and temperature (20C) in a room supplied with HEPA filtered air. Prior to printing the pins were cleaned by ultra-sonication in water and if necessary 50mM potassium hydroxide. A test print of all pins was carried out by printing Cy3 streptavidin conjugate dye in printing buffer onto epoxide slides and printing 100 features per pin. When all pins were printing consistent size spots for more than 80 features they were accepted as ready to print. The MicroGrid II was then programmed for the array print run, with a grid layout file prepared to allow duplicate printing atrandom within a patch. At the end of the print run the slides were removed following the program instructions in preparation for post processing. Slides dessicated overnight and then transferred to a humid chamber (45% humidity, 42C) for 8 hours. The slides were then washed in 0.2% SDS (room temperature) for 2 minutes with agitation before being rinsed in three water washes (30seconds, 30 seconds, 1 minute) with agitation. After these steps the slides were then incubated in water at 50C for 20 minutes before spin drying. The slides were then stored in a desiccator in the dark ready for use.
 
 
Submission date Mar 18, 2010
Last update date Mar 19, 2010
Contact name Zhangrui Cheng
E-mail(s) zcheng@rvc.ac.uk
Phone 01707666663
Organization name Royal Veterinary College
Department Production and Population Health
Lab Reproduction
Street address Hawkshead Lane
City Hatfield
State/province Hertfordshire
ZIP/Postal code AL9 7TA
Country United Kingdom
 
Samples (20) GSM523940, GSM523941, GSM523942, GSM523943, GSM523944, GSM523945 
Series (1)
GSE20953 Expression data from broiler chickens in response to the combined stressors of catching, transport and slaughter
Relations
Alternative to GPL8861

Data table header descriptions
ID
oligo ID
GB_ACC GenBank accession number
SEQUENCE probe sequence
SPOT_ID
Name

Data table
ID oligo ID GB_ACC SEQUENCE SPOT_ID Name
1 landing lights --landing lights
2 landing lights --landing lights
3 RIGG00061 AJ719513.1 AGTAGCTCTTCAGGTAATATCTTTAGTCCACAGCAGTCAAGTGGCCATCTAAAATCCCCAACTCCTCCAC "Gallus gallus mRNA for hypothetical protein, clone 2p6"
4 RIGG00332 AJ720994.1 ATGGACATCGAACGCGTCAACATCGCTTTCAACTACGACATGCCCGAGGATTCCGACACCTACCT "Gallus gallus mRNA for hypothetical protein, clone 32b9"
5 RIGG00059 AJ719500.1 CCTTGACTTGGAGTTTTATCCTCTCAGTATGAGTGTGCATGGCTATCTGTTGTGATAAAGGAGATCTGGG "Gallus gallus mRNA for hypothetical protein, clone 2o2"
6 RIGG00330 AJ720984.1 TTCTGAGATGTTCGGGAGAGAGGACCGATTTCTCAATCATTATGCGAACATGCGCTGGTTTGGTGGTGTG "Gallus gallus mRNA for hypothetical protein, clone 31o2"
7 RIGG00248 AJ720468.1 GCAGGATGTTGAATAAGTCTGATTTGGTAACCATGCTTCAAAAGTGTGTGGAAATTCTTGTGTCATCTCCTGG "Gallus gallus mRNA for hypothetical protein, clone 18f16"
8 RIGG00141 AJ719893.1 ACTCTTCAATGAAATGCTTCAGAGGGATTTTGGTGTCAGAATTTATAAAGCACTGATTTCCCTCCCAGAGAG "Gallus gallus partial mRNA for hypothetical protein, clone 7o17"
9 RIGG00153 AJ719971.1 ACTTATCAGCTGGAGAGACTATATGCCAGGTCTGCTTTCAGATATTCCCAAATAATGAGCAACTGGAGCAG "Gallus gallus mRNA for hypothetical protein, clone 8o12"
10 RIGG00236 AJ720432.1 TGTGCATTCTCCTGGAGAACAGTTGTTGATTGACAGGTCCTCTTATATATCCATACAGTGGAGAAGCAGTTTATT "Gallus gallus mRNA for hypothetical protein, clone 17i17"
11 RIGG00343 AJ721038.1 TACTTCAATCTCATCTCCTCGGTGGACCCCAAATTCCTGACGCTCACCAAAGTGGACGAGCAGATCTATG "Gallus gallus mRNA for hypothetical protein, clone 33c16"
12 RIGG00048 AJ719424.1 TCACTCCGAACTGTCTTGGATTTTAGGACCGGTATGCTCCATCACTTTATAACTGGAATGTACATGGTATT "Gallus gallus mRNA for hypothetical protein, clone 2c16"
13 RIGG00249 AJ720482.1 TAAATAACCAGCAGGGCTAAGGTGACTGTTAGTAGCCATCAGGTCTATTGAAAGCATGTGAGAGCAGCAA "Gallus gallus mRNA for hypothetical protein, clone 18k24"
14 RIGG00142 AJ719894.1 TGCAGAAGGCAGATACCCAAAATCGTTAATAGGGAAGAACTTCTGTGCCTATCTGTTGGAGCTGAGGAAC "Gallus gallus mRNA for hypothetical protein, clone 7o22"
15 RIGG00060 AJ719503.1 CTCACAGCAAAGAAGCTTGAAGAAGATATGTAACAGGGTGAAATGGACACGTGATGAGGATGAAAAGCTAAAG "Gallus gallus partial mRNA for hypothetical protein, clone 2o13"
16 RIGG00331 AJ720992.1 AGGCATGCAGGTCAAAATGTTTCGCATACGTTTTCAACCAACTAAAGGATTTGGGCAGCATCTGAGTTTA "Gallus gallus mRNA for hypothetical protein, clone 32a22"
17 RIGG00155 AJ719977.1 CGTGGAAGAGATGAAGAGCAAGAAATTCCTATTGAGTATCTGGGAGAAGCTTCCTACAAACATGAAAAGCT "Gallus gallus mRNA for hypothetical protein, clone 9a8"
18 RIGG00238 AJ720446.1 TGTAGAGGCTGTGTCTGTAAGATAGCATTGTATTTTCATGTCATTTTCTGTCCTGTGTATTTGCCAGCCTC "Gallus gallus mRNA for hypothetical protein, clone 17m23"
19 RIGG00344 AJ721043.1 AGGACTCCTCCAAGCCTCAAAGCGACTTTTGGATGAAGGTGGATTCCTAAAAGACTGAAACAGAATAGAC "Gallus gallus mRNA for hypothetical protein, clone 33e6"
20 RIGG00049 AJ719427.1 AGTCCAAGGCTCTAGGAGAAAGCTGATGGTTATTGAGAGTGATGCTTCTTGTATATTGTCAATGTGATTCCT "Gallus gallus mRNA for hypothetical protein, clone 2d1"

Total number of rows: 21888

Table truncated, full table size 2822 Kbytes.




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