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Platform GPL10337 Query DataSets for GPL10337
Status Public on Dec 31, 2010
Title RayBio Homo sapiens 0.1K apoptosis array
Technology type antibody
Distribution custom-commercial
Organism Homo sapiens
Manufacturer RayBiotech, Inc.
Manufacture protocol see manufacturer's website
Catalog number AAH-APO-1
Support nylon
Coating unknown
Description Principle of the Assay
With RayBio® Human Apoptosis Antibody Array kit, researchers can now simultaneously detect the relative level of 43 apoptosis related proteins in cell lysates. By monitoring the changes in protein levels in different experimental model systems, researchers can study pathway activation without spending excess time and effort in performing immunoprecipitations and/or Western Blotting.
With RayBio® Human Apoptosis Antibody Array kit, each of the capture antibodies is printed on the membranes, and then treated or untreated cell lysate is added into antibody array membranes. After extensive washing, the membranes are incubated with a cocktail of biotin-conjugated anti-apoptotic protein antibodies. After incubation with HRP-Streptavidin, the signals are visualized by chemiluminescence
Web link
Contributor(s) Spadavecchia S, Driscoll Carroll K, Palmeri D, Lukac DM
Submission date Apr 17, 2010
Last update date Dec 31, 2010
Contact name David M. Lukac
Phone 973 972-4868
Organization name Rutgers Univ./NJ Medical School
Department Microbiology, Biochemistry and Molecular Genetics
Street address 225 Warren St.; ICPH E350C; PO Box 1709
City Newark
State/province NJ
ZIP/Postal code 07101
Country USA
Samples (4) GSM534203, GSM534204, GSM534205, GSM534206
Series (1)
GSE21375 Convergence of KSHV Reactivation with EBV latency and cellular growth mediated by the Notch signaling pathway in co-infected cells.

Data table header descriptions
Description protein targeted by antibody

Data table
ID Description PT_ACC SPOT_ID
Pos a Positive Control a --Positive Control a
Pos b Positive Control b --Positive Control b
CD40 a CD40 a ABI49511.1
CD40 b CD40 b ABI49511.1
IGFBP-1 a Insulin-like growth factor-binding protein 1 a AAA52783.1
IGFBP-1 b Insulin-like growth factor-binding protein 1 b AAA52783.1
TNF-R2 a Tumor Necrosis Factor-Receptor 2 a BAA89055.1
TNF-R2 b Tumor Necrosis Factor-Receptor 2 b BAA89055.1
Pos c Positive Control c --Positive Control c
Pos d Positive Control d --Positive Control d
CD40L a CD40 Ligand a NP_000065.1
CD40L b CD40 Ligand b NP_000065.1
IGFBP-2 a Insulin-like growth factor-binding protein 2 a AAA03246.1
IGFBP-2 b Insulin-like growth factor-binding protein 2 b AAA03246.1
TNF-alpha a Tumor Necrosis Factor Alpha a NP_000585.2
TNF-alpha b Tumor Necrosis Factor Alpha b NP_000585.2
Neg a Negative Control a --Negative Control a
Neg b Negative Control b --Negative Control b
cIAP2 a Cellular Inhibitor of Apoptosis 2 a NP_001156.1
cIAP2 b Cellular Inhibitor of Apoptosis 2 b NP_001156.1

Total number of rows: 112

Table truncated, full table size 5 Kbytes.

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp

Supplementary data files not provided

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