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Platform GPL1055

Query DataSets for GPL1055

Status

Public on Mar 03, 2004

Title

NIAID - Hsbc

Technology type

spotted oligonucleotide

Distribution

non-commercial

Organism

Description

Printing by NIAID / Microarray Research Facility
Surface type: non-adsorptive, epoxy-coated glass
Attachment: covalent
Array description: The custom microarrays are manufactured by the Microarray Research Facility of the National Institute of Allergy and Infectious Diseases (NIH/NIAID). Microarrays are printed with an Omnigrid robotic arrayer by Genomic Solutions (http://www.genomicsolutions.com ) that is fitted with a printhead and 48 SMP3 print tips from Telechem (http://www.arrayit.com ). The human microarray (Hsbc) probe set is based on a subset of the library of 70mer oligonucleotides from Qiagen's Human Genome Oligo Set V2.0 ( http://oligos.qiagen.com/arrays/omad.php ). A comprehensive gene list is also available at http://madb.niaid.nih.gov/
Oligonucleotide probes are distributed into 384-well polypropylene plates (Genetix, 384 micro array) and dissolved to a normalized 25 micromolar concentration in print buffer (150mM sodium phosphate, pH 8.5, 4% sodium sulfate, 0.0001% N-lauroylsarcosine). The Hsbc microarray spot densities are 205 microns (center-to-center). The microarrays are printed on MWG Epoxy Slides (MWG Biotech Inc., # 1150-000000) and treated by a method that is an adaptation of the manufacturer's instructions to allow higher throughput processing.

Submission date

Mar 01, 2004

Last update date

Dec 28, 2005

Contact name

Michael Wilson

E-mail(s)

mwilson@ambion.com

Phone

301-496-4287

URL

http://madb.niaid.nih.gov/

Organization name

National Institute of Allergy and Infectious Diseases

Department

Division of Intramural Research

Lab

Research Technologies Branch / Microarray Research Facility

Street address

50 South Drive, Room 5509

City

Bethesda

State/province

MD

ZIP/Postal code

20892

Country

USA

Data table header descriptions
ID	NIH mAdb well id plus replicate number
MADB_WELL_ID	NIH mAdb plate-well identifier for oligo sample
OLIGO_ID	Oligo identifier
GENE	Gene Name
UniGene	UniGene Identifier Build #Hs.166
DESCRIPTION	Gene Description
SPOT_ID	spot identifier

Data table
ID	MADB_WELL_ID	OLIGO_ID	GENE	UniGene	DESCRIPTION	SPOT_ID
1310604_1	1310604	MRF3_50-a12			Tamara prelabeled MRF3_50 Control_4	Tamara prelabeled MRF3_50 Control_4
1310628_1	1310628	MRF3_50-c12			Tamara prelabeled MRF3_50 Control_4	Tamara prelabeled MRF3_50 Control_4
1310652_1	1310652	MRF1_50-e12			MRF1_50 Control_4	MRF1_50 Control_4
1310676_1	1310676	MRF2_50-g12			MRF2_50 Control_4	MRF2_50 Control_4
1310598_1	1310598	AMB_6F			AMB_6F Control_3 Ambion spiked standard	AMB_6F Control_3 Ambion spiked standard
1310622_1	1310622	Alien_10			Alien_10 Control_3 Stratagene spiked standard	Alien_10 Control_3 Stratagene spiked standard
1310646_1	1310646	Empty/Unknown			EmptyWell	EmptyWell
1310670_1	1310670	Empty/Unknown			EmptyWell	EmptyWell
1173596_1	1173596	H200000106	ADM	441047	adrenomedullin	ADM adrenomedullin
1173620_1	1173620	H200000130	ISL1	505	ISL1 transcription factor, LIM/homeodomain, (islet-1)	ISL1 ISL1 transcription factor, LIM/homeodomain, (islet-1)
1173644_1	1173644	H200000154	LGALS3	411701	lectin, galactoside-binding, soluble, 3 (galectin 3)	LGALS3 lectin, galactoside-binding, soluble, 3 (galectin 3)
1173668_1	1173668	H200000178	THRA	724	thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene homolog, avian)	THRA thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene homolog, avian)
1173590_1	1173590	H200000100	OPRD1	372	opioid receptor, delta 1	OPRD1 opioid receptor, delta 1
1173614_1	1173614	H200000124	ATF3	460	activating transcription factor 3	ATF3 activating transcription factor 3
1173638_1	1173638	H200000148	APOB	280226	apolipoprotein B (including Ag(x) antigen)	APOB apolipoprotein B (including Ag(x) antigen)
1173662_1	1173662	H200000172	CSTB	695	cystatin B (stefin B)	CSTB cystatin B (stefin B)
1173980_1	1173980	H200000486	KLKB1	1901	kallikrein B, plasma (Fletcher factor) 1	KLKB1 kallikrein B, plasma (Fletcher factor) 1
1174004_1	1174004	H200000510	SRY	1992	sex determining region Y	SRY sex determining region Y
1174028_1	1174028	H200000534	PTGER2	2090	prostaglandin E receptor 2 (subtype EP2), 53kDa	PTGER2 prostaglandin E receptor 2 (subtype EP2), 53kDa
1174052_1	1174052	H200000558	PROL3	2207	proline rich 3	PROL3 proline rich 3

Total number of rows: 19200

Table truncated, full table size 2260 Kbytes.

Download family	Format
SOFT formatted family file(s)	SOFT
MINiML formatted family file(s)	MINiML

Supplementary data files not provided

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