Oligonucleotides are synthesized directly on glass slides using standard phosphoramidite chemistry. Photo-lithography, driven by micro-mirror technology, is used to control the precise location of photo-generated acid production from an acid precursor. The acid removes a protection group (which prevents monomer oligomerization) on the terminal monomer. Only the deprotected spots can react with a new monomer. Uniquely, MYcroarray’s proprietary technology allows synthesis in liquid phase without physical barriers, masks or ink jets.
Circular features are ~55um in diameter. 4,280 E. coli genes are surveyed. The number of unique probe sequences per gene is either three (n = 3723), two (n =352) or one (n = 205). Consequently, there are 12,078 unique probe sequences surveying E. coli genes on the array. All probe sequences are replicated 6 times, so there are 72,468 E. coli probes on the array. In addition, there are 1,440 proprietary QC probe sequences on the array, 224 are for assessing hybridization efficiency. 8,012 potential feature locations contain no probe sequence.
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