GEO help: Mouse over screen elements for information.
Status
Public on Nov 20, 2010
Title
A-UMCU-Y16k-1.3
Technology type
spotted oligonucleotide
Distribution
non-commercial
Organism
Saccharomyces cerevisiae
Manufacturer
Holstege Lab, UMC Utrecht, The Netherlands
Manufacture protocol
1. Oligos are resuspended and adjusted to 10µM in 150mM phosphatebuffer, pH8.5 in Genetix 384-wells plates 2. Array production is done using a Biorobotics MicroGridII spotter located in a low dust cleanroom (ISO7/ downflow ISO5) at 18C, at 48% humidity 3. Slide postprocessing is carried out according to CodeLink protocol: Printed slides are kept at 20C, 75% humidity for 24 hours 4. Residual reactive groups are blocked using pre-warmed blocking solution (50 mM ethanolamine, 0.1 M Tris (pH 9) at 50C for 30 min; slides are rinsed thoroughly with milliQ water; slides are washed with 4x SSC, 0.1% SDS (pre-warmed to 50C) for 30 min at 50C on a shaker 5. Slides are rinsed with milliQ water, centrifuged to dry and stored dessicated at room temperature.
Support
glass
Web link
http://microarrays.holstegelab.nl
Contributor(s)
van Leenen d , Groot Koerkamp M , Lijnzaad P , Bakker L , Sluiters E , van Hooff S , Kemmeren P , Holstege F
Submission date
Nov 19, 2010
Last update date
Mar 26, 2013
Contact name
Patrick Kemmeren
Organization name
UMC Utrecht
Department
Department of Molecular Cancer Research
Lab
Holstege Lab
Street address
Universiteitsweg 100
City
Utrecht
State/province
Utrecht
ZIP/Postal code
3584 CG
Country
Netherlands
Samples (4152)
GSM630089 , GSM630090 , GSM630091 , GSM630092 , GSM630093 , GSM630094
GSM630095 ,
GSM630096 ,
GSM630097 ,
GSM630098 ,
GSM630099 ,
GSM630100 ,
GSM630101 ,
GSM630102 ,
GSM630103 ,
GSM630104 ,
GSM630105 ,
GSM630106 ,
GSM630107 ,
GSM630108 ,
GSM630109 ,
GSM630110 ,
GSM630111 ,
GSM630112 ,
GSM630113 ,
GSM630114 ,
GSM630115 ,
GSM630116 ,
GSM630117 ,
GSM630118 ,
GSM630119 ,
GSM630120 ,
GSM630121 ,
GSM630122 ,
GSM630123 ,
GSM630124 ,
GSM630125 ,
GSM630126 ,
GSM630127 ,
GSM630128 ,
GSM630129 ,
GSM630130 ,
GSM630131 ,
GSM630132 ,
GSM630133 ,
GSM630134 ,
GSM630135 ,
GSM630136 ,
GSM630137 ,
GSM630138 ,
GSM630139 ,
GSM630140 ,
GSM630141 ,
GSM630142 ,
GSM630143 ,
GSM630144 ,
GSM630145 ,
GSM630146 ,
GSM630147 ,
GSM630148 ,
GSM630149 ,
GSM630150 ,
GSM630151 ,
GSM630152 ,
GSM630153 ,
GSM630154 ,
GSM630155 ,
GSM630156 ,
GSM630157 ,
GSM630158 ,
GSM630159 ,
GSM630160 ,
GSM630161 ,
GSM630162 ,
GSM630163 ,
GSM630164 ,
GSM630165 ,
GSM630166 ,
GSM630167 ,
GSM630168 ,
GSM630169 ,
GSM630170 ,
GSM630171 ,
GSM630172 ,
GSM630173 ,
GSM630174 ,
GSM630175 ,
GSM630176 ,
GSM630177 ,
GSM630178 ,
GSM630179 ,
GSM630180 ,
GSM630181 ,
GSM630182 ,
GSM630183 ,
GSM630184 ,
GSM630185 ,
GSM630186 ,
GSM630187 ,
GSM630188 ,
GSM630189 ,
GSM630190 ,
GSM630191 ,
GSM630192 ,
GSM630193 ,
GSM630194 ,
GSM630195 ,
GSM630196 ,
GSM630197 ,
GSM630198 ,
GSM630199 ,
GSM630200 ,
GSM630201 ,
GSM630202 ,
GSM630203 ,
GSM630204 ,
GSM630205 ,
GSM630206 ,
GSM630207 ,
GSM630208 ,
GSM630209 ,
GSM630210 ,
GSM630211 ,
GSM630212 ,
GSM630213 ,
GSM630214 ,
GSM630215 ,
GSM630216 ,
GSM630217 ,
GSM630218 ,
GSM630219 ,
GSM630220 ,
GSM630221 ,
GSM630222 ,
GSM630223 ,
GSM630224 ,
GSM630225 ,
GSM630226 ,
GSM630227 ,
GSM630228 ,
GSM630229 ,
GSM630230 ,
GSM630231 ,
GSM630232 ,
GSM630233 ,
GSM630234 ,
GSM630235 ,
GSM630236 ,
GSM630237 ,
GSM630238 ,
GSM630239 ,
GSM630240 ,
GSM630241 ,
GSM630242 ,
GSM630243 ,
GSM630244 ,
GSM630245 ,
GSM630246 ,
GSM630247 ,
GSM630248 ,
GSM630249 ,
GSM630250 ,
GSM630251 ,
GSM630252 ,
GSM630253 ,
GSM630254 ,
GSM630255 ,
GSM630256 ,
GSM630257 ,
GSM630258 ,
GSM630259 ,
GSM630260 ,
GSM630261 ,
GSM630262 ,
GSM630263 ,
GSM630264 ,
GSM630265 ,
GSM630266 ,
GSM630267 ,
GSM630268 ,
GSM630269 ,
GSM630270 ,
GSM630271 ,
GSM630272 ,
GSM630273 ,
GSM630274 ,
GSM630275 ,
GSM630276 ,
GSM630277 ,
GSM630278 ,
GSM630279 ,
GSM630280 ,
GSM630281 ,
GSM630282 ,
GSM630283 ,
GSM630284 ,
GSM630285 ,
GSM630286 ,
GSM630287 ,
GSM630288 ,
GSM630289 ,
GSM630290 ,
GSM630291 ,
GSM630292 ,
GSM630293 ,
GSM630294 ,
GSM630295 ,
GSM630296 ,
GSM630297 ,
GSM630298 ,
GSM630299 ,
GSM630300 ,
GSM630301 ,
GSM630302 ,
GSM630303 ,
GSM630304 ,
GSM630305 ,
GSM630306 ,
GSM630307 ,
GSM630308 ,
GSM630309 ,
GSM630310 ,
GSM630311 ,
GSM630312 ,
GSM630313 ,
GSM630314 ,
GSM630315 ,
GSM630316 ,
GSM630317 ,
GSM630318 ,
GSM630319 ,
GSM630320 ,
GSM630321 ,
GSM630322 ,
GSM630323 ,
GSM630324 ,
GSM630325 ,
GSM630326 ,
GSM630327 ,
GSM630328 ,
GSM630329 ,
GSM630330 ,
GSM630331 ,
GSM630332 ,
GSM630333 ,
GSM630334 ,
GSM630335 ,
GSM630336 ,
GSM630337 ,
GSM630338 ,
GSM630339 ,
GSM630340 ,
GSM630341 ,
GSM630342 ,
GSM630343 ,
GSM630344 ,
GSM630345 ,
GSM630346 ,
GSM630347 ,
GSM630348 ,
GSM630349 ,
GSM630350 ,
GSM630351 ,
GSM630352 ,
GSM630353 ,
GSM630354 ,
GSM630355 ,
GSM630356 ,
GSM630357 ,
GSM630358 ,
GSM630359 ,
GSM630360 ,
GSM630361 ,
GSM630362 ,
GSM630363 ,
GSM630364 ,
GSM630365 ,
GSM630366 ,
GSM630367 ,
GSM630368 ,
GSM630369 ,
GSM630370 ,
GSM630371 ,
GSM630372 ,
GSM630373 ,
GSM630374 ,
GSM630375 ,
GSM630376 ,
GSM630377 ,
GSM630378 ,
GSM630379 ,
GSM630380 ,
GSM630381 ,
GSM630382 ,
GSM630383 ,
GSM630384 ,
GSM630385 ,
GSM630386 ,
GSM630387 ,
GSM630388 ,
GSM630389 ,
GSM630390 ,
GSM630391 ,
GSM630392 ,
GSM630393 ,
GSM630394 ,
GSM630395 ,
GSM630396 ,
GSM630397 ,
GSM630398 ,
GSM630399 ,
GSM630400 ,
GSM630401 ,
GSM630402 ,
GSM630403 ,
GSM630404 ,
GSM630405 ,
GSM630406 ,
GSM630407 ,
GSM630408 ,
GSM630409 ,
GSM630410 ,
GSM630411 ,
GSM630412 ,
GSM630413 ,
GSM630414 ,
GSM630415 ,
GSM630416 ,
GSM630417 ,
GSM630418 ,
GSM630419 ,
GSM630420 ,
GSM630421 ,
GSM630422 ,
GSM630423 ,
GSM630424 ,
GSM630425 ,
GSM630426 ,
GSM630427 ,
GSM630428 ,
GSM630429 ,
GSM630430 ,
GSM630431 ,
GSM630432 ,
GSM630433 ,
GSM630434 ,
GSM630435 ,
GSM630436 ,
GSM630437 ,
GSM630438 ,
GSM630439 ,
GSM630440 ,
GSM630441 ,
GSM630442 ,
GSM630443 ,
GSM630444 ,
GSM630445 ,
GSM630446 ,
GSM630447 ,
GSM630448 ,
GSM630449 ,
GSM630450 ,
GSM630451 ,
GSM630452 ,
GSM630453 ,
GSM630454 ,
GSM630455 ,
GSM630456 ,
GSM630457 ,
GSM630458 ,
GSM630459 ,
GSM630460 ,
GSM630461 ,
GSM630462 ,
GSM630463 ,
GSM630464 ,
GSM630465 ,
GSM630466 ,
GSM630467 ,
GSM630468 ,
GSM630469 ,
GSM630470 ,
GSM630471 ,
GSM630472 ,
GSM630473 ,
GSM630474 ,
GSM630475 ,
GSM630476 ,
GSM630477 ,
GSM630478 ,
GSM630479 ,
GSM630480 ,
GSM630481 ,
GSM630482 ,
GSM630483 ,
GSM630484 ,
GSM630485 ,
GSM630486 ,
GSM630487 ,
GSM630488 ,
GSM630489 ,
GSM630490 ,
GSM630491 ,
GSM630492 ,
GSM630493 ,
GSM630494 ,
GSM630495 ,
GSM630496 ,
GSM630497 ,
GSM630498 ,
GSM630499 ,
GSM630500 ,
GSM630501 ,
GSM630502 ,
GSM630503 ,
GSM630504 ,
GSM630505 ,
GSM630506 ,
GSM630507 ,
GSM630508 ,
GSM630509 ,
GSM630510 ,
GSM630511 ,
GSM630512 ,
GSM630513 ,
GSM630514 ,
GSM630515 ,
GSM630516 ,
GSM630517 ,
GSM630518 ,
GSM630519 ,
GSM630520 ,
GSM630521 ,
GSM630522 ,
GSM630523 ,
GSM630524 ,
GSM630525 ,
GSM630526 ,
GSM630527 ,
GSM630528 ,
GSM630529 ,
GSM630530 ,
GSM630531 ,
GSM630532 ,
GSM630533 ,
GSM630534 ,
GSM630535 ,
GSM630536 ,
GSM630537 ,
GSM630538 ,
GSM630539 ,
GSM630540 ,
GSM630541 ,
GSM630542 ,
GSM630543 ,
GSM630544 ,
GSM630545 ,
GSM630546 ,
GSM630547 ,
GSM630548 ,
GSM630549 ,
GSM630550 ,
GSM630551 ,
GSM630552 ,
GSM636333 ,
GSM636334 ,
GSM636335 ,
GSM636336 ,
GSM636337 ,
GSM636338 ,
GSM636339 ,
GSM636340 ,
GSM636341 ,
GSM636342 ,
GSM636343 ,
GSM636344 ,
GSM636345 ,
GSM636346 ,
GSM636347 ,
GSM636348 ,
GSM636349 ,
GSM636350 ,
GSM636351 ,
GSM636352 ,
GSM636353 ,
GSM636354 ,
GSM636355 ,
GSM636356 ,
GSM636357 ,
GSM636358 ,
GSM636359 ,
GSM636360 ,
GSM636361 ,
GSM636362 ,
GSM636363 ,
GSM636364 ,
GSM636365 ,
GSM636366 ,
GSM636367 ,
GSM636368 ...
Accession list truncated, click here to browse through all related public accessions You can also download a list of all accessions here
Series (34)
GSE25644
Expression profiling of yeast kinase and phosphatase knockouts
GSE25909
Gene deletion expression profiles of yeast chromatin modifiers
GSE30148
GS003: Tight cooperation between Mot1p and NC2β in regulating genome-wide transcription, repression of transcription following heat shock induction and genetic interaction with SAGA.
GSE30168
Expression profiles of the NuB4/HAT-B complex and histone knockouts
GSE33097
Deletion mutant analysis of established glucose signaling and metabolic pathway members in Saccharomyces cerevisiae
GSE33098
Glucose-depletion time-course experiment in Saccharomyces cerevisiae wild-type cells.
GSE33099
Glucose signaling
GSE33929
Slx5_Slx8_Genome_Stability
GSE39861
Gene deletion expression profiles of SAGA, TREX2 and Sem1
GSE39903
Expression profiles of histone point mutants
GSE40254
Ctk1 + CTD mutants
GSE42215
yeast wt pool
GSE42217
yeast wt pool - plate reader
GSE42240
yeast wt-matA pool
GSE42241
yeast wt-matA pool - plate reader
GSE42526
Compendium of deletion mutant gene expression profiles, 784 non-responsive mutants [hs1990]
GSE42527
Compendium of deletion mutant gene expression profiles, 700 responsive mutants [hs1991]
GSE42528
Compendium of deletion mutant gene expression profiles
GSE42536
Transcription profiling by array of yeast single and double deletion mutants of gene-specific transcription factors
GSE43120
RNAPII CTD dataset
GSE45115
Expression profiling of 376 wildtypes to assess day-to-day variance
GSE47429
Kaplan2013: High-Resolution, Quantitative Genetic Analysis of RNA Polymerase II
GSE54527
wt yeast expression changes in low phosphate conditions
GSE54528
Heatshock-Coldshock
GSE54539
wt expression changes in deletion strain-conditioned media
GSE55373
BP001-002: The genomic landscape of compensatory evolution
GSE75725
XB001: Shortening of membrane lipid acyl chains compensates for phosphatidylcholine deficiency in choline‐auxotroph yeast
GSE81479
HSF1-expression: Molecular mechanisms that distinguish TFIID housekeeping from regulatable SAGA promoters
GSE81480
MOT1-expression: Molecular mechanisms that distinguish TFIID housekeeping from regulatable SAGA promoters
GSE81481
HSF1 and MOT1-expression and binding: Molecular mechanisms that distinguish TFIID housekeeping from regulatable SAGA promoters
GSE98205
Promoter DNA sequence guides factors that position the +1 nucleosome and facilitate TBP binding [array]
GSE98260
Promoter DNA sequence guides factors that position the +1 nucleosome and facilitate TBP binding
GSE128936
A role for the RNA Polymerase II-CTD phosphatase, FCP1 in the oxidative stress response
GSE137261
A balancing act: Interactions within NuA4/TIP60 regulate picNuA4 function in Saccharomyces cerevisiae and humans
Data table header descriptions
ID
primary identifier
METACOLUMN
metagrid column position
METAROW
metagrid row position
COLUMN
grid column position
ROW
grid row position
PROBE_ID
alternative spot identifier
SUPPLIER_ID
original supplier id, e.g. from agilent or operon
REPORTER_GROUP
class the reporter belongs to, e.g. controls or genes
SEQUENCE
nucleotide sequence
SYSTEMATIC_NAME
systematic name of the gene
ORF
ORF Name
SGD_ID
Saccharomyces Genome Database Identifier
GENE_SYMBOL
Gene symbol for the gene
SYNONYMS
Synonyms for the gene, semicolon separated
ORGANISM
organism
PRIMARY_DB
primay external database identifier, format is <extdb>:<identifier>
SPOT_ID
Data table
ID
METACOLUMN
METAROW
COLUMN
ROW
PROBE_ID
SUPPLIER_ID
REPORTER_GROUP
SEQUENCE
SYSTEMATIC_NAME
ORF
SGD_ID
GENE_SYMBOL
SYNONYMS
ORGANISM
PRIMARY_DB
SPOT_ID
1
1
1
1
1
ECAA000001
ex_control
TTTATCGGCTGTAATAGAGCTGGTGCCGTTTTTAGATAAACAGAAGGCAAAAGAAACCCACCATTCAATA
2
1
1
2
1
SCAB005915
YPL060W_01
gene
AACAGAAACGAATTAATGCTGTTAGGGATAAGATACGCCATAGGAATGCTTTCGTTGGGAGGCGCTCTAT
YPL060W
YPL060W
S000005981
LPE10
LPE10
Saccharomyces cerevisiae
sgd:S000005981
3
1
1
3
1
SCAB005939
YPL084W_01
gene
CAATTTCAAAATCTCAAAATAAGTGCCGGCAGTGATTTACCTCAAGGACCCGGTATTCCACCAAGAACTT
YPL084W
YPL084W
S000006005
BRO1
ASI6;BRO1;LPF2;NPI3;VPS31
Saccharomyces cerevisiae
sgd:S000006005
4
1
1
4
1
QCAA000002
qc_control
--qc_control
5
1
1
5
1
SCAB005963
YPL108W_01
gene
GAATAAAATGAAGAGGGAGCTTGAAGAGTTGGAATATATGATCGAGCGATGTCGTGGGCATGTATTATAA
YPL108W
YPL108W
S000006029
YPL108W
Saccharomyces cerevisiae
sgd:S000006029
6
1
1
6
1
ECAA000007
ex_control
AATGGATTGCGCTTCAGTAAAAAACATTTAACTGATGAGTTGTACCGGTTTAATGCTTTTACATCAGTTC
7
1
1
7
1
SCAB005987
YPL132W_01
gene
TCACCAGTGAAGTATCACAAATTTTACCGTGGAAATTCGTTCCTCAACAACGTGAGGTTTACGTTCTGCC
YPL132W
YPL132W
S000006053
COX11
COX11;LPI13;PSO7
Saccharomyces cerevisiae
sgd:S000006053
8
1
1
8
1
SCAB006295
YPR147C_01
gene
AAATGACAGCGGCACTTCGTTATATTCCACCATTGGCTCATGTGGTATCATTGTTCAGTTATATTTTCTT
YPR147C
YPR147C
S000006351
YPR147C
Saccharomyces cerevisiae
sgd:S000006351
9
1
1
9
1
SCAB006319
YPR167C_01
gene
AGTGGAACCTGCACATCGTGCCTACAAAGAGCTACATATAAGTGCTGTGTTTACTGGTAGAAGAAAATCA
YPR167C
YPR167C
S000006371
MET16
MET16
Saccharomyces cerevisiae
sgd:S000006371
10
1
1
10
1
ECAA000008
ex_control
AGTACGAGCCCAAAAAATCAAAAAAGGATTAAAACGCCTGCAAGAGAAGCATTATTTAGACAGACAACTG
11
1
1
11
1
SCAB006343
YPR191W_01
gene
ACTTATCCCCTGCAATAAATTACACAAAGTTAAAGAATGCCGTCCAAAATGAATCTGTTTCCAGCCCAAT
YPR191W
YPR191W
S000006395
QCR2
COR2;QCR2;UCR2
Saccharomyces cerevisiae
sgd:S000006395
12
1
1
12
1
QCAA000001
qc_control
--qc_control
13
1
1
13
1
ECAA000006
ex_control
GACAGGAAAAGACACTTCTGTCATTGTCAGCTTAATTGTATTAGCTGCTTTGTTATTTTCCTTAAGGGCT
14
1
1
14
1
SCAB006301
YPR153W_01
gene
CCATAGAAAGAAGGCTGGAAGTGTATGGATATTCGTCATGTACGTCTTATACGGAGGCGTTCAAGGCTTG
YPR153W
YPR153W
S000006357
YPR153W
Saccharomyces cerevisiae
sgd:S000006357
15
1
1
15
1
SCAB006325
YPR173C_01
gene
TCCATGCGTATTGACCAAGGAAGATTATAGAACTTTAGGTGCAATGACTGAAGGTTATTCCGGGAGTGAT
YPR173C
YPR173C
S000006377
VPS4
CSC1;DID6;END13;GRD13;VPL4;VPS4;VPT10
Saccharomyces cerevisiae
sgd:S000006377
16
1
1
16
1
SCAB006349
YPR197C_01
gene
ACTTCTATTTAAAGTAGTTCCAAGATGTCATTTCTTTTTACGAAGCGCAGCCGTTTATCAACCATTCTAA
YPR197C
YPR197C
S000006401
YPR197C
Saccharomyces cerevisiae
sgd:S000006401
17
1
1
17
1
QCAA000001
qc_control
--qc_control
18
1
1
18
1
ECAA000001
ex_control
TTTATCGGCTGTAATAGAGCTGGTGCCGTTTTTAGATAAACAGAAGGCAAAAGAAACCCACCATTCAATA
19
1
1
1
2
SCAB005215
YNR016C_01
gene
GAAGATTGAACGAAGAATATTTGATTAAAAGGTTGAGCCATCAGGTAGGCGAAGCATCAAGATTAGAAAA
YNR016C
YNR016C
S000005299
ACC1
ABP2;ACC1;FAS3;MTR7
Saccharomyces cerevisiae
sgd:S000005299
20
1
1
2
2
SCAB005524
YOR073W_01
gene
AATCGTCGCCTTTACTTGACATTACAAATAAATCGGAGAATAAGAAAAAGTCAACAAGAACTAAAAAATT
YOR073W
YOR073W
S000005599
SGO1
SGO1;YOR29-24
Saccharomyces cerevisiae
sgd:S000005599
Total number of rows: 15552 Table truncated, full table size 2654 Kbytes .
Supplementary data files not provided