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Status
Public on Jun 16, 2011
Title
Fusarium graminearum febit in situ oligonucleotide array
Technology type
in situ oligonucleotide
Distribution
non-commercial
Organism
Fusarium graminearum Manufacturer
febit biomed
Manufacture protocol
DNA-Synthesis took place in the Geniom One Instrument - a benchtop device that utilizes microfluidics for light activated oligonucleotide synthesis - at febit biomed facilities (Heidelberg, Germany). One probe for each gene was synthesized on the array. Blank probes consisting of only one T were used for background correction. Probes were 50-mers.
Support
glass
Coating
unknown
Description
Geniom One, all-in-one instrument for sample loading, hybridization, washing, staining, and detection of biochips.
Contributor(s)
Carapito R Submission date
Jun 14, 2011
Last update date
Jun 16, 2011
Contact name
Raphael Carapito
Organization name
Ecole Supérieure de Biotechnologie de Strasbourg
Street address
Bd Sébastien Brandt
City
Illkirch
ZIP/Postal code
67400
Country
France
Samples (16)
GSM741353 , GSM741920 , GSM741921 , GSM741922 , GSM741923 , GSM741924
Series (1)
GSE29973 Transcriptional responses of Fusarium graminearum to plant cell wall substrates
Data table header descriptions ID ORF ORF Name
SEQUENCE oligonucleotide probe sequence
SPOT_ID
Data table ID ORF SEQUENCE SPOT_ID F2.1-MM-control
GCAGTGCTGCCAAAACCATGAGTGA
--F2.1-MM-control
F2.1-PM-control
GCAGTGCTGCCATAACCATGAGTGA
--F2.1-PM-control
Chloramphenicol
CAAACTGAAACGTTTTCATCGCTCT
--Chloramphenicol
Kanamycin
GCGGACCGCTATCAGGACATAGCGT
--Kanamycin
Blank-control
--Blank-control
FG00001
FG00001 TTTTATCACACATCTACGGACCTTTTGCCTGCCGACATCCGCGGTTTCTT
FG00002
FG00002 CGTTGTGTTATCGGTACAAGGACTTCGTGCTTCAATTTGGAGGTTTGCCC
FG00003
FG00003 AACGAATAGAAGGCGTCTCGGAAGACGCGTTGGTCACTTTATCAATGCTG
FG00004
FG00004 ATGTACTACGGTGACCTGAAGAGGAACTTTCTCGCTCAAGGAGAAACTGG
FG00005
FG00005 AATGTTATACCTTGCATACCTGTCCACCTGTTGGTGCTGCTGGACTGACT
FG00006
FG00006 TAACTTCTACGGGAAAGAAGCCCAGCGCAAAGTTTCGTAACCGCCATATC
FG00007
FG00007 CCAAGGAATGCAAAGTGTGTGGACCTGATTAGAGCTGTGGAGAAGGAACA
FG00008
FG00008 GTGGGGAGAAGATCGATGAAGCATGGTGGTTTGCTACCAAGAACGGTTTT
FG00009
FG00009 CTTGGACGTGAACTGGAATGTAAATGGCCCTGTCAAAGGTGATCCGGTTT
FG00010
FG00010 GTCCGTAAGAATGCTTTCGGGAGATCTCCACATGAGTCTGGGAAACTCAA
FG00011
FG00011 ACCCTTGTAGGTGGATATTGCATAAGAGACCCATGTGAACAGACCCATGC
FG00012
FG00012 TTGCCATGCCTACCAAGGACTTTAAGGTGTCGCGGTTTGAAGCTTTCAAC
FG00013
FG00013 ATTGGAGCAGATAGATGAGGTCTTTAATGACCATGCGGCCCAGGAAGAGT
FG00014
FG00014 ATTCTTGACTACTATGGTCTACTCACTGTCAGCCGAGAGGGTTGGTTGAC
FG00015
FG00015 TATACTTGCAGTGGAGAACTCAACTGCTACGGAGCTCATGTCGAGAGACT
11644 773 Kbytes .Supplementary data files not provided
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