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Platform GPL14184 Query DataSets for GPL14184
Status Public on Oct 01, 2011
Title OSU-CCC Human and Mouse MicroRNA Microarray Version 4.0
Technology type spotted oligonucleotide
Distribution non-commercial
Organisms Homo sapiens; Mus musculus
Manufacturer Microarray Shared Resource, Comprehensive Cancer Center, The Ohio State University (OSU-CCC)
Manufacture protocol Manufacured using GE Healthcare's CodeLink (TM) 3-D Gel Matrix Technology. CodeLink(tm) Activated Slides are designed to covalently immoblize amine-modified DNA for microarrays. The slides are coated with a 3-D surface chemistry comprised of a long-chain, hydrophilic polymer containing amine-reactive groups. This polymer is covalently crosslinked to itself and to the surface of the slide. The crosslinked polymer, combined with end-point attachment, orients the immobilized DNA, and holds it away from the surface of the slide. The 1x printing buffer consists of 50 mM sodium phosphate at pH 8.5. Codelink slides are manufactured by SurModics and were formally sold as 3D-Link(tm) Activated Slides.   Microarrays are printed with an Omnigrid robotic arrayer by Genomic Solutions (http://www.genomicsolutions.com ) that is fitted with a printhead and 16 SMP3 print tips from Telechem (http://www.arrayit.com). The microRNA microarray probe set is based on 40mer oligonucleotides from Sigma-Genosys.   Oligonucleotide probes are distributed into 384-well polypropylene plates (Genetix, 384 micro array) and dissolved in print buffer (150mM sodium phosphate, pH 8.5, 4% sodium sulfate, 0.0001% N-lauryl sarcosyl) to a normalized 25 micromolar concentration. The miRNA microarray spot densities are 200 microns (center-to-center). The microarrays are printed on Codelink Activated Slide (PN300011)) and treated by a method that is an adaptation of the manufacturer's instructions to allow higher throughput processing.
 
 
Submission date Aug 23, 2011
Last update date Oct 01, 2011
Contact name Robert Scott Hudson
Organization name NIH
Department NCI
Lab The Laboratory of Human Carcinogenesis
Street address National Cancer Institute Room Blgd.37/3044
City Bethesda
State/province MD
ZIP/Postal code 20852
Country USA
 
Samples (19) GSM785505, GSM785506, GSM785507, GSM785508, GSM785509, GSM785510 
Series (1)
GSE31620 MicroRNA-1 is a candidate tumor suppressor and prognostic marker
Relations
Alternative to GPL13716

Data table header descriptions
ID
PROBE NAME
miRNA_ID
SEQUENCE
SPOT_ID

Data table
ID PROBE NAME miRNA_ID SEQUENCE SPOT_ID
1 hsa-mir-125b2-A hsa-mir-125b2-a \5AmMC6\ACCAGACTTTTCCTAGTCCCTGAGACCCTAACTTGTGAGG
2 hsa-mir-125b2-A hsa-mir-125b2-a \5AmMC6\ACCAGACTTTTCCTAGTCCCTGAGACCCTAACTTGTGAGG
3 hsa-mir-181a2-P hsa-mir-181a2-p \5AmMC6\GAAAAAACCACTGACCGTTGACTGTACCTTGGGGTCCTTA
4 hsa-mir-181a2-P hsa-mir-181a2-p \5AmMC6\GAAAAAACCACTGACCGTTGACTGTACCTTGGGGTCCTTA
5 hsa-mir-127-A hsa-mir-127-a \5AmMC6\TCGGATCCGTCTGAGCTTGGCTGGTCGGAAGTCTCATCAT
6 hsa-mir-128a-A hsa-mir-128a-a \5AmMC6\ACATTTCTCACAGTGAACCGGTCTCTTTTTCAGCTGCTTC
7 hsa-mir-128a-A hsa-mir-128a-a \5AmMC6\ACATTTCTCACAGTGAACCGGTCTCTTTTTCAGCTGCTTC
8 hsa-mir-181c-A hsa-mir-181c-a \5AmMC6\TGCCAAGGGTTTGGGGGAACATTCAACCTGTCGGTGAGTT
9 hsa-mir-128b-A hsa-mir-128b-a \5AmMC6\TCACAGTGAACCGGTCTCTTTCCCTACTGTGTCACACTCC
10 hsa-mir-128b-A hsa-mir-128b-a \5AmMC6\TCACAGTGAACCGGTCTCTTTCCCTACTGTGTCACACTCC
11 hsa-mir-182-5p-A hsa-mir-182-5p-a \5AmMC6\TTTGGCAATGGTAGAACTCACACTGGTGAGGTAACAGGAT
12 hsa-mir-182-5p-A hsa-mir-182-5p-a \5AmMC6\TTTGGCAATGGTAGAACTCACACTGGTGAGGTAACAGGAT
13 hsa-mir-129-1-P hsa-mir-129-1-p \5AmMC6\CCTCTCAACAGTAGTCAGGAAGCCCTTACCCCAAAAAGTA
14 hsa-mir-129-1-P hsa-mir-129-1-p \5AmMC6\CCTCTCAACAGTAGTCAGGAAGCCCTTACCCCAAAAAGTA
15 hsa-mir-130a-A hsa-mir-130a-a \5AmMC6\GTCTGCACCTGTCACTAGCAGTGCAATGTTAAAAGGGCAT
16 hsa-mir-132-A hsa-mir-132-a \5AmMC6\TGTGGGAACTGGAGGTAACAGTCTACAGCCATGGTCGCCC
17 hsa-mir-132-A hsa-mir-132-a \5AmMC6\TGTGGGAACTGGAGGTAACAGTCTACAGCCATGGTCGCCC
18 hsa-mir-593-P hsa-mir-593-p \5AmMC6\AAGGAGTGGTGCTGGGTTTGTCTCTGCTGGGGTTTCTCCT
19 hsa-mir-593-P hsa-mir-593-p \5AmMC6\AAGGAGTGGTGCTGGGTTTGTCTCTGCTGGGGTTTCTCCT
20 hsa-mir-563-P hsa-mir-563-p \5AmMC6\AGCAAAGAAGTGTGTTGCCCTCTAGGAAATGTGTGTTGCT

Total number of rows: 6572

Table truncated, full table size 569 Kbytes.




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