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Status
Public on Nov 05, 2004
Title
Hopkins Tag Array
Technology type
spotted oligonucleotide
Distribution
non-commercial
Organism
Saccharomyces cerevisiae
Description
Comprehensive array of all 6018 UpTag/DnTag pairs designed for incorporation into yeast deletion strains by the Yeast Deletion Project (June 2002). Custom-designed negative and positive controls comprise almost half of the 215x105 array. Keywords = yeast, deletion, knockout, tag, barcode, synthetic, control, oligonucleotide
Contributor(s)
Yuan DS , Boeke JD
Citation(s)
15994458
Submission date
Sep 13, 2004
Last update date
Jul 20, 2005
Contact name
Xuewen Pan
E-mail(s)
xpan2@jhmi.edu
Phone
410-955-2477
Organization name
Johns Hopkins University School of Medicine
Department
Department of Molecular Biology and Genetics
Lab
Boeke Lab
Street address
733 N. Broadway, Room 331
City
Baltimore
State/province
MD
ZIP/Postal code
21205
Country
USA
Samples (218)
GSM30549 , GSM30550 , GSM30551 , GSM30552 , GSM30553 , GSM30554
GSM30555 ,
GSM30556 ,
GSM30557 ,
GSM30558 ,
GSM30559 ,
GSM30560 ,
GSM30561 ,
GSM30570 ,
GSM30571 ,
GSM30572 ,
GSM30573 ,
GSM30574 ,
GSM30575 ,
GSM30576 ,
GSM30577 ,
GSM30578 ,
GSM30579 ,
GSM61945 ,
GSM61948 ,
GSM61949 ,
GSM61950 ,
GSM61951 ,
GSM81980 ,
GSM81981 ,
GSM81982 ,
GSM81983 ,
GSM81984 ,
GSM81985 ,
GSM81987 ,
GSM81988 ,
GSM81989 ,
GSM81990 ,
GSM81991 ,
GSM81992 ,
GSM81993 ,
GSM81994 ,
GSM81995 ,
GSM81996 ,
GSM81997 ,
GSM81998 ,
GSM81999 ,
GSM82000 ,
GSM82001 ,
GSM82002 ,
GSM82003 ,
GSM82004 ,
GSM82005 ,
GSM82006 ,
GSM82007 ,
GSM82008 ,
GSM82009 ,
GSM82010 ,
GSM82011 ,
GSM82012 ,
GSM82013 ,
GSM82014 ,
GSM82015 ,
GSM82016 ,
GSM82017 ,
GSM82018 ,
GSM82019 ,
GSM82020 ,
GSM82021 ,
GSM82022 ,
GSM82023 ,
GSM82024 ,
GSM82025 ,
GSM82026 ,
GSM82027 ,
GSM82028 ,
GSM82029 ,
GSM82030 ,
GSM82031 ,
GSM82032 ,
GSM82033 ,
GSM82034 ,
GSM82035 ,
GSM82036 ,
GSM82037 ,
GSM82038 ,
GSM82039 ,
GSM82040 ,
GSM82041 ,
GSM82042 ,
GSM82043 ,
GSM82044 ,
GSM82045 ,
GSM82046 ,
GSM82047 ,
GSM82048 ,
GSM82049 ,
GSM82050 ,
GSM82051 ,
GSM82052 ,
GSM82053 ,
GSM82054 ,
GSM82055 ,
GSM82056 ,
GSM82057 ,
GSM82058 ,
GSM82059 ,
GSM82060 ,
GSM82061 ,
GSM82062 ,
GSM82063 ,
GSM82064 ,
GSM82065 ,
GSM82066 ,
GSM82067 ,
GSM82068 ,
GSM82069 ,
GSM82070 ,
GSM82071 ,
GSM82072 ,
GSM82073 ,
GSM82074 ,
GSM82075 ,
GSM82076 ,
GSM82077 ,
GSM82078 ,
GSM82079 ,
GSM82080 ,
GSM82081 ,
GSM82082 ,
GSM82083 ,
GSM82084 ,
GSM82085 ,
GSM82086 ,
GSM82087 ,
GSM82088 ,
GSM82089 ,
GSM82090 ,
GSM82091 ,
GSM82092 ,
GSM82093 ,
GSM82094 ,
GSM82095 ,
GSM82096 ,
GSM82097 ,
GSM82098 ,
GSM82099 ,
GSM85470 ,
GSM85471 ,
GSM94607 ,
GSM138553 ,
GSM138554 ,
GSM138555 ,
GSM138556 ,
GSM138557 ,
GSM138558 ,
GSM138559 ,
GSM138560 ,
GSM138561 ,
GSM246442 ,
GSM246443 ,
GSM246444 ,
GSM246445 ,
GSM246446 ,
GSM246447 ,
GSM246448 ,
GSM246449 ,
GSM246450 ,
GSM246451 ,
GSM246452 ,
GSM246453 ,
GSM246454 ,
GSM246455 ,
GSM246456 ,
GSM246457 ,
GSM246458 ,
GSM246459 ,
GSM246460 ,
GSM246461 ,
GSM246462 ,
GSM246463 ,
GSM246464 ,
GSM246465 ,
GSM246466 ,
GSM246467 ,
GSM246468 ,
GSM246469 ,
GSM246470 ,
GSM246471 ,
GSM246472 ,
GSM246473 ,
GSM246474 ,
GSM246475 ,
GSM246476 ,
GSM246477 ,
GSM246478 ,
GSM246479 ,
GSM246480 ,
GSM246481 ,
GSM246482 ,
GSM246483 ,
GSM254974 ,
GSM254975 ,
GSM319211 ,
GSM319212 ,
GSM319213 ,
GSM319214 ,
GSM319215 ,
GSM319216 ,
GSM319217 ,
GSM319218 ,
GSM319219 ,
GSM319220 ,
GSM524004 ,
GSM524005 ,
GSM524006 ,
GSM946550 ,
GSM946551
Series (16)
GSE1758
CDC102 synthetic lethality
GSE1759
LCD1-overexpression synthetic lethality
GSE1760
TUB1 synthetic haploinsufficiency
GSE1765
SML1 synthetic lethality
GSE1838
dSLAM
GSE2832
Improved statistical analysis of budding yeast TAG microarrays
GSE3574
A DNA integrity network in the yeast Saccharomyces cerevisiae
GSE4144
Synthetic lethality/slow growth with acs2-Ts8
GSE5973
Trivalent arsenic inhibits the functions of chaperonin complex
GSE9771
A comprehensive synthetic genetic interaction network governing yeast histone acetylation and deacetylation
GSE12733
Phenotypic profiling of adaptation to oxidative stress
GSE20961
Functional Variomics Library testing for drug resistance in yeast
GSE38601
The Fun30 ATP-dependent nucleosome remodeler promotes resection of DNA double-strand break ends
Data table header descriptions
ID
Serial identifier for probe
ROW
Row number in the array as scanned with GenePix scanner
COLUMN
Column number in the array as scanned with GenePix scanner
TAGTYPE
Code for whether tag is 5' (Up) or 3' (Dn) relative to the open reading frame (ORF)
PROBE
Code for singleton probes arrayed in ORF order (ArrA, ArrB), five-fold replicate probes arrayed in randomized order (Rpts), systematic mutations arrayed across the center of the array (Muts), negative controls (NegT), or probes peripheral to the array as specified by the manufacturer (Edge)
ORF
Systematic ORF name (from SGD, Feb 2003)
GENE
Standard gene name (SGD) (or ORF if not available)
SEQUENCE
DNA sequence of probe (includes custom-designed sequences for 193 YA* and YM* ORFs missing DnTags)
SGDID
Unique ORF identifier from SGD; 'S000000000' denotes missing value
SPOT_ID
spot identifier; ('YQL' ORFs denote custom-designed sequences; 'NegA', 'NegB', 'PosA', 'PosB' denote proprietary sequences specified by the manufacturer)
Data table
ID
ROW
COLUMN
TAGTYPE
PROBE
ORF
GENE
SEQUENCE
SGDID
SPOT_ID
1
213
104
Up
ArrA
YAL001C
TFC3
ACTATATGTGAAGGCATGGC
S000000001
2
211
104
Up
ArrA
YAL002W
VPS8
ATACTGACAGCACGCATGGC
S000000002
3
209
104
Up
ArrA
YAL003W
EFB1
GACATATCAGCATACATGGC
S000000003
4
207
104
Up
ArrA
YAL004W
YAL004W
TATGGCACGGCAGACATTCC
S000002136
5
205
104
Up
ArrA
YAL005C
SSA1
AGGCATACTACACAGATTCC
S000000004
6
203
104
Up
ArrA
YAL007C
ERP2
GAGTGATCCATACACATTCC
S000000005
7
201
104
Up
ArrA
YAL008W
FUN14
ATGAACTTGCGCTCAATTCC
S000000006
8
199
104
Up
ArrA
YAL009W
SPO7
GCCAGATCATCAATAGGCAC
S000000007
9
197
104
Up
ArrA
YAL010C
MDM10
CACTATATGTCAACAGGCAC
S000000008
10
195
104
Up
ArrA
YAL011W
SWC1
CAGTAATCAGAAGCTCGCAC
S000000009
11
193
104
Up
ArrA
YAL012W
CYS3
TAGCACACGGTCGAATTTCC
S000000010
12
191
104
Up
ArrA
YAL013W
DEP1
GATACGCGCCAATCTCTATA
S000000011
13
189
104
Up
ArrA
YAL014C
SYN8
CCGCATACCGAATTGCTATA
S000000012
14
187
104
Up
ArrA
YAL015C
NTG1
GCGCAGAAGGCAATGCTATA
S000000013
15
185
104
Up
ArrA
YAL016W
TPD3
CGCCTAAGACCGGATAAAGC
S000000014
16
183
104
Up
ArrA
YAL017W
PSK1
GCACATCTGACAACAGGCGA
S000000015
17
181
104
Up
ArrA
YAL018C
YAL018C
CATACACGTAAAGTGCGCGA
S000000016
18
179
104
Up
ArrA
YAL019W
FUN30
TGCACAGCGACAATACGCGA
S000000017
19
177
104
Up
ArrA
YAL020C
ATS1
CGATTCGATAAATGACGCGA
S000000018
20
175
104
Up
ArrA
YAL021C
CCR4
CAGTCGATCACAAGCGAGCA
S000000019
Total number of rows: 22575 Table truncated, full table size 1494 Kbytes .
Supplementary data files not provided