This cDNA Yeast Chip was developed in-house at NIEHS. A complete listing of the 6,218 open reading frames on this chip is available at the following website: https://tools.niehs.nih.gov/maps/index.cfm cDNA microarray chips were prepared as previously described (DeRisi, Penland et al. 1996; Hauser, Vingron et al. 1998). The spotted cDNAs were derived from a collection of primers of yeast ORFs (Research Genetics, Carlsbad, CA). These primers were used to amplify yeast cDNAs from genomic DNA in a 100µl PCR reaction mixture. A sample of the PCR products (10 ul) was separated on 2% agarose gels to ensure quality of the amplifications. The remaining PCR products were purified by ethanol precipitation, resuspended in ArrayIt Spotting Solution buffer (Telechem, San Jose CA) and spotted onto poly-L-lysine coated glass slides using a modified, robotic DNA arrayer (Beecher Instruments, Bethesda MD).
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