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Status
Public on Jul 01, 2012
Title
Agilent_024261 OGT_Streptococcus uberis_3780 features_8X15K_v1.0
Technology type
spotted oligonucleotide
Distribution
custom-commercial
Organism
Streptococcus uberis 0140J
Manufacturer
Oxford Gene Technology, Begbroke Science Park, Sandy Lane, Yarnton, Oxford, OX5 1PF, United Kingdom
Manufacture protocol
S. uberis 0140J DNA microarray encompassed the whole bacterial genome, composed of 3780 features, with optimised 60mer oligonucleotide probes printed in triplicate. Two separate probes were designed to match each individual gene determined from the S. uberis 0140J genome annotation with regions of homology discounted. Arrays were printed on glass microscope slides following described protocols (Hughes, Mao et al, Nature Biotechnol 2001, 9(4)342-7).
Description
DesignID : 024261
Submission date
Oct 24, 2011
Last update date
Jul 01, 2012
Contact name
Sharon Egan
E-mail(s)
sharon.egan@nottingham.ac.uk
Organization name
University of Nottingham
Department
School of Veterinary Medicine and Sciences
Street address
Sutton Bonington Campus
City
Sutton Bonington
State/province
Leicestershire
ZIP/Postal code
LE125RD
Country
United Kingdom
Samples (12)
GSM821482 , GSM821483 , GSM821484 , GSM821485 , GSM821486 , GSM821487
Series (2)
GSE33186
Gene expression based analysis of Streptococcus uberis 0140J and vru (sub0144) mutant
GSE36677
Gene expression-based analysis of Streptococcus uberis 0140J and the MtuR (sub0472) mutant
Data table header descriptions
ID
FeatureNum
Row
Location of probe
Column
Location of probe
ProbeName
Individual S. uberis gene and probe number combined
ORF
ORF Name
SEQUENCE
Sequence of oligonucleotide probe
SPOT_ID
Data table
ID
Row
Column
ProbeName
ORF
SEQUENCE
SPOT_ID
1
1
1
GE_BrightCorner
--GE_BrightCorner
2
1
2
DarkCorner
--DarkCorner
3
1
3
DarkCorner
--DarkCorner
4
1
4
SUB1489_1
SUB1489
AACTATACCAGAAGTTTTACGTCCGTATATGGGCGGAGCAGAAATGATTTCTCCAAAGTA
5
1
5
SUB0921_1
SUB0921
ATACCAAATCGCTAAAAGAGGTATCCCTGTTAAACTTTATGAAATGCGTGGGGTTAAAGC
6
1
6
SUB1128_1
SUB1128
TTTTGATTGTAGATGACTTGTTAGCAACTGGAGGAACGGTCAAAGCCACTATTGAAATGG
7
1
7
SUB0538_2
SUB0538
GGATTGATCTGAAACAATATGGTCCAAATCCTCAAAAAGAGGAAGCTTTCCGACGTTATT
8
1
8
SUB0760_2
SUB0760
AGCATTATTGTTTACTGGTACCATAGAAGAGAACATCAAATATGGTAAAGAGCAAGCAAC
9
1
9
SUB0344_2
SUB0344
CTAATGAATGAAGGCAATGTAGTGGCAGATTTTGATAGTGACTTAATCGGTGACGGAAGT
10
1
10
SUB1784_2
SUB1784
AAATAATGCAATTTATACTTGCAATTACCGTGACTCTATTTGCAACTGTCGGTATGAAAA
11
1
11
SUB0993_2
SUB0993
TCGTTTAGTAGAAGGTGATCATAATATTGATTGTAAAATTGATGGTTTTGGTGCCATGAA
12
1
12
SUB0874_1
SUB0874
TAGTCAGGCAAATGCCGCGGCTTACATGGCTTTAATTGAATCAGGTGATACAGTCTTAGG
13
1
13
SUB0786_1
SUB0786
CGTAGAAGAATTACTAGGCTTTGAGTTACCTTTTGATAAAGAAAGGAACTTAAAGAATGG
14
1
14
SUB1398_1
SUB1398
CACTTGTTTTACTTATTCTGATTGCCTTGTCTGGAAGTATTTTGGGTGGAGAATTTTTAA
15
1
15
SUB1665_2
SUB1665
AAGTTATTCAGAGGGTAATGATCCAAACCGCTATGTGGTTGTCAGTCTTCAAAAACAATA
16
1
16
SUB1190_2
SUB1190
AAAGTTATACAGGAGGATTGCCTCTTTACCCGGTCAGTGAAAAACCGAAAAGAAGTGGGG
18
1
18
SUB1383_2
SUB1383
TAGCACAAGAATTCATCAGTAATACCTTAAAACATGCCAAAGCAAGTCAATTGGAAGTAT
19
1
19
SUB1787_1
SUB1787
ATCACAGTAGTGTCAAAGCATATAAGGACTCAGAAAAGAGATTGAAAGATCTTCCTCAAA
20
1
20
SUB1762_2
SUB1762
TTTAAGAGATGAACCTACAGACTGGGTTTCAAAATTCGGTGGGAATGCTTGGGCACCATT
21
1
21
SUB0436_1
SUB0436
TAGAAAATTTATCCATTAACTATGGTGCTATTGAGGCTGTTAAAGAGGTTTCTTTCCGTG
Total number of rows: 15120 Table truncated, full table size 1324 Kbytes .
Supplementary data files not provided