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Platform GPL14800 Query DataSets for GPL14800
Status Public on Oct 27, 2011
Title IBMT Campylobacter jejuni Halolink fusion-protein array
Technology type spotted peptide or protein
Distribution custom-commercial
Organism Campylobacter jejuni
Manufacturer IBMT
Manufacture protocol Spotting was performed with a Qarray2
 
Description Spotted fusion protein.
HaloTag-fusion proteins derived from Campylobacter jejuni and expressed in KRX E.coli overexpression strains.
We incorporated a fusion tag prior to our genes of interest and attached the expressed fusion proteins covalently on microarrays. This enhances the specific binding of the proteins compared to nitrocellulose.
Platform Halolink Arrays (Promega), spotting performed at IBMT.
 
Submission date Oct 27, 2011
Last update date Oct 27, 2011
Contact name Sebastian Hoppe
E-mail(s) sebastian.hoppe@izi-bb.fraunhofer.de
Organization name Fraunhofer Institute for Cell Therapy and Immunology
Department Bioanalytics and Biosensorics
Lab Molecular Bioengineering
Street address Am Mühlenberg 13
City Potsdam
ZIP/Postal code 14476
Country Germany
 
Samples (5) GSM823536, GSM823537, GSM823539, GSM823541, GSM823543
Series (1)
GSE33295 Detection of immunogenic proteins from bacteria

Data table header descriptions
ID
Block subarray on the slide
spot Spot number within a subarray
Name Common Protein name or sample name
PROTEIN_ACC UniprotKB/Swiss-Prot
SPOT_ID

Data table
ID Block spot Name PROTEIN_ACC SPOT_ID
1 1 1 HaloTag-GST HaloTag-GST
2 1 2 HaloTag-GST HaloTag-GST
3 1 3 HaloTag-GST HaloTag-GST
4 1 4 HaloTag-GST HaloTag-GST
5 1 5 HaloTag-GST HaloTag-GST
6 1 6 HaloTag-GST HaloTag-GST
7 1 7 cjaA P94643 cjaA
8 1 8 hisJ Q46125 hisJ
9 1 9 flaA P56963 flaA
10 1 10 flaC P96747 flaC
11 1 11 peb1 Q0P9X8 peb1
12 1 12 gapA Q0P8L1 gapA
13 1 13 pyrC Q0PBP6 pyrC
14 1 14 argC Q9PIS0 argC
15 1 15 pal Q46123 pal
16 1 16 pseB Q0P8W4 pseB
17 1 17 KRX extract KRX extract
18 1 18 PBS PBS
19 1 19 HaloTag-GST HaloTag-GST
20 1 20 HaloTag-GST HaloTag-GST

Total number of rows: 192

Table truncated, full table size 5 Kbytes.




Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp

Supplementary data files not provided

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