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Status
Public on Dec 20, 2011
Title
CNIO Genomics Unit Custom Multi-Species miRNA Microarray
Technology type
spotted oligonucleotide
Distribution
custom-commercial
Organisms
Caenorhabditis elegans ; Drosophila melanogaster ; Danio rerio ; Homo sapiens ; Mus musculus ; Rattus norvegicus Manufacturer
Genomics Unit. CNIO (Spanish National Cancer Research Centre). Madrid. Spain.
Manufacture protocol
NCode Multi-Species miRNA V2 probeset (Invitrogen, cat. # MIRMPS2-01) was printed on Nexterion epoxy E slides (Schott) by following manufacturer’s recommendations. The probeset contains a number of internal and negative control probes and includes 1,140 oligonucleotides that target all of the known mature miRNAs in the Sanger miRBase Sequence Database, Release 9.0 complementary to C. elegans, Drosophila, zebrafish, mouse, rat, and human miRNAs.
Submission date
Dec 19, 2011
Last update date
Dec 20, 2011
Contact name
Luis LOMBARDIA
E-mail(s)
llombardia@cnio.es Organization name
CNIO (Spanish National Cancer Center)
Department
Molecualr Pathology Programme
Lab
Molecular Diagnostics Unit
Street address
Melchor Fernandez Almagro, 3
City
MADRID
State/province
Madrid
ZIP/Postal code
28029
Country
Spain
Samples (6)
GSM851103 , GSM851104 , GSM851105 , GSM851106 , GSM851107 , GSM851108 Series (1)
GSE34564 MicroRNA-22 upregulation by vitamin D mediates its protective action against colon cancer.
Data table header descriptions ID Column The column location of probe within array
Row The row location of probe within array
Reporter Name Name for probes on the microarray
CLONE_ID miRBase accession number of miRNA
Reporter BioSequence Polymer Type The polymer type of probes on the microarray
Reporter Group [role] Column to identify probes as experimental or control.
Reporter Control Type Column to specify the type of control for control spots.
SEQUENCE The sequence of the mature miRNA
miRNA_LIST SPOT_ID
Data table ID Column Row Reporter Name CLONE_ID Reporter BioSequence Polymer Type Reporter Group [role] Reporter Control Type SEQUENCE miRNA_LIST SPOT_ID 2
1
1
NcodeControl00-2
DNA
Control
control_spike_calibration
--control_spike_calibration
4
2
1
mmu-miR-294
MIMAT0000372
DNA
Experimental
aaagugcuucccuuuugugugu
mmu-miR-294
6
3
1
hsa-miR-193a
MIMAT0000459
DNA
Experimental
aacuggccuacaaagucccag
hsa-miR-193a
8
4
1
hsa-miR-224
MIMAT0000281
DNA
Experimental
caagucacuagugguuccguuua
hsa-miR-224
10
5
1
hsa-miR-302d
MIMAT0000718
DNA
Experimental
uaagugcuuccauguuugagugu
hsa-miR-302d
12
6
1
dme-miR-8
MIMAT0000113
DNA
Experimental
uaauacugucagguaaagauguc
dme-miR-8
14
7
1
dme-miR-92b
MIMAT0000358
DNA
Experimental
aauugcacuagucccggccugc
dme-miR-92b
16
8
1
hsa-miR-368
MIMAT0000720
DNA
Experimental
acauagaggaaauuccacguuu
hsa-miR-368
18
9
1
dme-miR-6
MIMAT0000111
DNA
Experimental
uaucacaguggcuguucuuuuu
dme-miR-6
20
10
1
hsa-miR-373*
MIMAT0000726
DNA
Experimental
gaagugcuucgauuuuggggugu
hsa-miR-373*
22
11
1
rno-miR-349
MIMAT0000599
DNA
Experimental
cagcccugcugucuuaaccucu
rno-miR-349
24
12
1
hsa-miR-34c
MIMAT0000686
DNA
Experimental
aggcaguguaguuagcugauugc
hsa-miR-34c
98
13
1
dme-miR-33
MIMAT0000342
DNA
Experimental
aggugcauuguagucgcauug
dme-miR-33
100
14
1
dme-miR-2c
MIMAT0000411
DNA
Experimental
uaucacagccagcuuugaugggc
dme-miR-2c
102
15
1
cel-miR-65
MIMAT0000037
DNA
Experimental
uaugacacugaagcguaaccgaa
cel-miR-65
104
16
1
cel-miR-60
MIMAT0000032
DNA
Experimental
uauuaugcacauuuucuaguuca
cel-miR-60
106
17
1
cel-miR-245
MIMAT0000301
DNA
Experimental
auugguccccuccaaguagcuc
cel-miR-245
108
18
1
cel-miR-358
MIMAT0000700
DNA
Experimental
caauugguaucccugucaagg
cel-miR-358
110
1
2
cel-miR-36
MIMAT0000007
DNA
Experimental
ucaccgggugaaaauucgcaug
cel-miR-36
112
2
2
hsa-miR-103
MIMAT0000101
DNA
Experimental
agcagcauuguacagggcuauga
hsa-miR-103
2592 206 Kbytes .Supplementary data files not provided
