University of Verona - Scientific and Technological Department - Plant Functional Genomics Centre
Manufacture protocol
Probes are directly synthesized on a 90k Combimatrix array by an in situ oligo synthesis system developed by CombiMatrix Corporation (Mukilteo, USA) using the CombiMatrix CustomArray synthesizer. Standard synthesis protocol provided by CombiMatrix was used.
Support
silicon
Coating
unknown
Description
18,883 specific oligos (35-40mer) were designed on Bean Gene Index Version 3 (Release 3.0. May 22, 2009) tentative consensus (TC) sequences and 11,205 specific oligos (35-40mer) were designed on Soybean UniGene Built #38 by using the program design OligoArray 2.1 (Rouillard et al. 2003). 141 oligonucleotides sequences provided by the manufacturer were used as quality and negative controls. The 90K BeanArrayTM 1.0 was designed with three replicates of each probe randomly distributed across the array to allow measurement of the variability within the array. All the procedures provided were performed as indicated by CombiMatrix protocols available at the CombiMatrix website (www.combimatrix.com).