W.M. Keck Center, University of Illinois Urbana-Champaign
Manufacture protocol
cDNAs were printed in 3xSSC/1.5M betaine on amino-silane coated slides.
Description
The 7,872 cDNAs used in this microarray were obtained from normalized and subtracted cattle placenta and spleen cDNA libraries (see Band et al., 2002 Anim. Biotechnol. 13:163-172; Everts et al., 2005 Vet. Immunol. Immunopathol. 105:235-245). Positive controls include beta actin (ACTB), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and hypoxanthine phosphoribosyltransferase (HPRT). Exogenous spiking controls are the soybean genes chlorophyll ab binding protein (CAB), Rubisco small chain 1 (RBS1), and major latex protein (MSG). Negative controls are Cot1 DNA, genomic DNA, spotting buffer, poly-A, and water. All PCR products were printed in duplicate on the array. This is an alternative version of GPL2108. Keywords: cDNA, cow, bovine, microarray
Feed restriction, but not L-carnitine infusion, affects the liver transcriptome with an evident and inhibition of energy production and sterol synthesis and increase of gluconeogenesis in mid-lactating dairy cows
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