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Status
Public on Oct 01, 2013
Title
S. cerevisiae flocculation array
Technology type
spotted oligonucleotide
Distribution
non-commercial
Organism
Saccharomyces cerevisiae Manufacturer
TCI University of Hanover
Manufacture protocol
Oligonucleotide design and synthesis: 70mer oligonucleotides with a 5’ aminolink were designed and synthesised by Operon (Cologne, Germany). Spotting protocol: To produce microarrays silylated slides were purchased from TeleChemTM International Inc. (Sunnyvale, CA, USA) and the printing of the oligonucleotides was performed by using an AffymetrixTM 417 arrayer. Oligonucleotide solutions were prepared in 96-well-plates containing each 30 µl. The concentration of the printed oligonucleotides was 50 µM in 1x ArrayItTM Microarray Spotting Solution Plus. After spotting, the slides were incubated at 80 °C for 2 h. Slides were blocked in SSC containing 1% BSA (Sigma) for 45 min and rinsed with water for 5 min.
Submission date
Apr 10, 2013
Last update date
Sep 11, 2013
Contact name
Frank Stahl
E-mail(s)
stahl@iftc.uni-hannover.de Phone
0511-7622968
Organization name
Universität Hannover
Department
Institut für Technische Chemie
Street address
Callinstr. 3
City
Hannover
ZIP/Postal code
30167
Country
Germany
Samples (80)
GSM1120081 , GSM1120082 , GSM1120083 , GSM1120084 , GSM1120085 , GSM1120086
GSM1120087 ,
GSM1120088 ,
GSM1120089 ,
GSM1120090 ,
GSM1120091 ,
GSM1120092 ,
GSM1120093 ,
GSM1120094 ,
GSM1120095 ,
GSM1120096 ,
GSM1120097 ,
GSM1120098 ,
GSM1120099 ,
GSM1120100 ,
GSM1120101 ,
GSM1120102 ,
GSM1120103 ,
GSM1120104 ,
GSM1120105 ,
GSM1120106 ,
GSM1120107 ,
GSM1120108 ,
GSM1120109 ,
GSM1120110 ,
GSM1120111 ,
GSM1120112 ,
GSM1120113 ,
GSM1120114 ,
GSM1120115 ,
GSM1120134 ,
GSM1120135 ,
GSM1120136 ,
GSM1120137 ,
GSM1120138 ,
GSM1120139 ,
GSM1120140 ,
GSM1120141 ,
GSM1120142 ,
GSM1120143 ,
GSM1120144 ,
GSM1120145 ,
GSM1120146 ,
GSM1120147 ,
GSM1120148 ,
GSM1120149 ,
GSM1120150 ,
GSM1120151 ,
GSM1120152 ,
GSM1120153 ,
GSM1120154 ,
GSM1120155 ,
GSM1120156 ,
GSM1120157 ,
GSM1120158 ,
GSM1120159 ,
GSM1120160 ,
GSM1120161 ,
GSM1120162 ,
GSM1120163 ,
GSM1120164 ,
GSM1120165 ,
GSM1120166 ,
GSM1120167 ,
GSM1120168 ,
GSM1120169 ,
GSM1120170 ,
GSM1120171 ,
GSM1120172 ,
GSM1120173 ,
GSM1120174 ,
GSM1120175 ,
GSM1120176 ,
GSM1120177 ,
GSM1120178 Series (6)
GSE45943 Comparison between all runs of industrial serial repitching 1
GSE45944 Comparison between all runs of industrial serial repitching 2
GSE45946 Comparison between all runs of industrial serial repitching 3
GSE45947 Comparison between all runs of industrial serial repitching 4
GSE45948 Comparison between stationary phase S. cerevisiae H155 cells, exponential phase cells and stressed cells
GSE45951 Serial industrial repitching and stress
Data table header descriptions ID BLOCK Sub-Grid
ROW Column of sub-grid
COLUMN Row of sub-grid
ACC_NO Accession number
GENE SYMBOL Abbreviation of gene name
DESCRIPTION Gene description
SEQUENCE Oligonucleotide sequence
ORF SPOT_ID
Data table ID BLOCK ROW COLUMN ACC_NO GENE SYMBOL DESCRIPTION SEQUENCE ORF SPOT_ID 1
1
1
1
null
null
null
--null
2
1
1
2
null
null
null
--null
3
1
1
3
null
null
null
--null
4
1
1
4
null
null
null
--null
5
1
1
5
null
null
null
--null
6
1
1
6
null
null
null
--null
7
1
2
1
null
null
null
--null
8
1
2
2
null
null
null
--null
9
1
2
3
null
null
null
--null
10
1
2
4
null
null
null
--null
11
1
2
5
null
null
null
--null
12
1
2
6
null
null
null
--null
13
1
3
1
null
null
null
--null
14
1
3
2
null
null
null
--null
15
1
3
3
null
null
null
--null
16
1
3
4
null
null
null
--null
17
1
3
5
null
null
null
--null
18
1
3
6
null
null
null
--null
19
1
4
1
null
null
null
--null
20
1
4
2
null
null
null
--null
864 79 Kbytes .Supplementary data files not provided
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