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Status
Public on Aug 22, 2013
Title
miRXplore TM Microarray (968)
Technology type
spotted DNA/cDNA
Distribution
commercial
Organisms
Homo sapiens ; Mus musculus ; Rattus norvegicus Manufacturer
Miltenyi Biotec GmbH, Cologne, Germany
Manufacture protocol
1.2 nl of 10 µM HPLC purified, 5’ amino-modified DNA oligonucleotides (Metabion) were spotted on aminopropyltrimethoxysilane / phenylendiisothiocyanate coated glass slides in quadruplicates using a customized piezoelectric spotter.
Description
The miRXplore TM Microarray is composed of 6428 DNA oligonucleotides with a sequence being reverse complement to the respective mature miRNA or control RNA, representing a total of 1510 miRNA sequences and 97 controls with all probes spotted in quadruplicates. The 1510 miRNA sequences covered sequence verified miRNAs of the species human (898) mouse (742) and rat (505) as well as viral miRNAs (148) as deposited in the miRBase sequence database version 17.0. The 97 positive and negative control probes were chosen as follows. Three sets of controls, miRControl 1 (four sequences used to assess efficiency and sensitivity of labelling), miRControl 2 (single sequence used to assess efficiency of small RNA enrichment) and miRControl 3 (43 sequences used to normalize signal intensities across different samples and arrays) were artificial sequences chosen not be present in any of the human, mouse, rat or viral genome. A fourth set of positive controls presented 36 positive small but non-miRNA sequences including probes e.g. for 5.1 sRNA, tRNAs, and U6 RNAs. A fifth set of oligonucleotides included 13 negative controls like different single or double mismatch, antisense, or scrambled miRNA sequences. Microarrays were manufactured by Miltenyi Biotec. Briefly, 1.2 nl of 10 µM HPLC purified, 5’ amino-modified DNA oligonucleotides (Metabion) were spotted on aminopropyltrimethoxysilane / phenylendiisothiocyanate coated glass slides in quadruplicates using a customized piezoelectric spotter.
Web link
http://www.miltenyibiotec.com Contributor(s)
Gerstmayer B , Bosio A Submission date
Aug 21, 2013
Last update date
Aug 22, 2013
Contact name
Silvia Rueberg
E-mail(s)
silvia@miltenyibiotec.de Organization name
Miltenyi Biotec GmbH
Department
Genomic Services
Street address
Friedrich-Ebert-Str. 68
City
Bergisch Gladbach
ZIP/Postal code
51429
Country
Germany
Samples (76)
GSM1215472 , GSM1215473 , GSM1215474 , GSM1215475 , GSM1215476 , GSM1215477
GSM1215478 ,
GSM1215479 ,
GSM1215480 ,
GSM1215481 ,
GSM1215482 ,
GSM1215483 ,
GSM1215484 ,
GSM1215485 ,
GSM1215486 ,
GSM1215487 ,
GSM1215488 ,
GSM1215489 ,
GSM1215490 ,
GSM1215491 ,
GSM1215492 ,
GSM1215493 ,
GSM1215494 ,
GSM1215495 ,
GSM1215496 ,
GSM1215497 ,
GSM1215498 ,
GSM1215499 ,
GSM1215500 ,
GSM1215501 ,
GSM1215502 ,
GSM1215503 ,
GSM1215504 ,
GSM1215505 ,
GSM1215506 ,
GSM1215507 ,
GSM1215508 ,
GSM1215509 ,
GSM1215510 ,
GSM1215511 ,
GSM1215512 ,
GSM1215513 ,
GSM1215514 ,
GSM1215515 ,
GSM1215516 ,
GSM1215517 ,
GSM1215518 ,
GSM1215519 ,
GSM1276464 ,
GSM1276465 ,
GSM1276466 ,
GSM1276467 ,
GSM2120750 ,
GSM2120751 ,
GSM2120752 ,
GSM2120753 ,
GSM2120754 ,
GSM2120755 ,
GSM2120756 ,
GSM2120757 ,
GSM2120758 ,
GSM2120759 ,
GSM2120760 ,
GSM2120761 ,
GSM2120762 ,
GSM2120763 ,
GSM2120764 ,
GSM2120765 ,
GSM2120766 ,
GSM2120767 ,
GSM2120768 ,
GSM2120769 ,
GSM2120770 ,
GSM2120771 ,
GSM2120772 ,
GSM2120773 Series (3)
GSE50194 Identification of candidate microRNAs in colorectal adenomas and adenocarcinomas
GSE52802 MicroRNA analysis of breast cancer cell lines
GSE80201 Ewing's sarcoma: an analysis of miRNA expression profiles and target genes in paraffin-embedded primary tumor tissue
Data table header descriptions ID stem-loop_acc Stem-loop_acc
mature_acc Mature_acc
name Name
miltenyi_id cDNA identifier at Miltenyi Biotec GmbH
miRNA_ID miRNA identifiers
SPOT_ID
Data table ID stem-loop_acc mature_acc name miltenyi_id miRNA_ID SPOT_ID 1
MI0000558 , MI0000829 , MI0000063 , MI0005453 , MI0005351 , MI0001172 MIMAT0000522 , MIMAT0000775 , MIMAT0000063 , MIMAT0004331 , MIMAT0004162 , MIMAT0001102 LET-7B
35816
LET-7B 2
MI0000064 , MI0000559 , MI0000560 , MI0000830 , MI0000831 , MI0005454 , MI0002445 , MI0001174 MIMAT0000776 , MIMAT0000523 , MIMAT0000064 , MIMAT0004332 , MIMAT0002151 , MIMAT0001104 LET-7C
35817
LET-7C 3
MI0000103 , MI0000739 , MI0000148 , MI0000886 , MI0002719 , MI0004735 , MI0002717 , MI0002723 , MI0002725 , MI0005283 , MI0005282 , MI0002720 , MI0002722 , MI0002721 , MI0002718 , MI0001270 , MI0016974 , MI0007610 MIMAT0000133 , MIMAT0000099 , MIMAT0000823 , MIMAT0002427 , MIMAT0003520 , MIMAT0002425 , MIMAT0002431 , MIMAT0002433 , MIMAT0004098 , MIMAT0002428 , MIMAT0002430 , MIMAT0002429 , MIMAT0002426 , MIMAT0001185 MIR-101-101C
35823
MIR-101-101C 4
MI0000266 , MI0000685 , MI0000841 , MI0002791 , MI0005007 , MI0002788 , MI0005273 , MI0002792 , MI0002790 MIMAT0000782 , MIMAT0000648 , MIMAT0000253 , MIMAT0002489 , MIMAT0003786 , MIMAT0002486 , MIMAT0004089 , MIMAT0002490 , MIMAT0002488 MIR-10A
35824
MIR-10A 5
MI0000442 , MI0000256 , MI0000891 , MI0005063 , MI0005287 , MI0002413 , MI0001277 , MI0001280 MIMAT0000827 , MIMAT0000246 , MIMAT0000421 , MIMAT0003849 , MIMAT0004101 , MIMAT0002119 , MIMAT0001190 MIR-122
35826
MIR-122 6
MI0000897 , MI0000446 , MI0000470 , MI0000152 , MI0000725 , MI0000896 , MI0002510 , MI0002575 , MI0004753 , MI0005457 , MI0002509 , MI0002574 , MI0002514 , MI0002579 , MI0002517 , MI0002581 , MI0005291 , MI0005292 , MI0002511 , MI0002513 , MI0002578 , MI0002512 , MI0002577 , MI MIMAT0000830 , MIMAT0000136 , MIMAT0000423 , MIMAT0002221 , MIMAT0003539 , MIMAT0002220 , MIMAT0002225 , MIMAT0002228 , MIMAT0004103 , MIMAT0002222 , MIMAT0002224 , MIMAT0002223 , MIMAT0002226 , MIMAT0002120 , MIMAT0001105 MIR-125B
35830
MIR-125B 7
MI0000471 , MI0000153 , MI0000898 , MI0004754 , MI0001244 MIMAT0000137 , MIMAT0000444 , MIMAT0000831 , MIMAT0004328 , MIMAT0003723 MIR-126-5P
35832
MIR-126-5P 8
MI0000473 , MI0000585 , MI0000637 , MI0000902 MIMAT0000601 , MIMAT0000544 , MIMAT0004605 , MIMAT0017120 MIR-129-3P
35835
MIR-129-3P 9
MI0000252 , MI0000473 , MI0000222 , MI0000585 , MI0000637 , MI0000902 , MI0005294 MIMAT0000600 , MIMAT0000209 , MIMAT0000242 , MIMAT0004105 MIR-129-5P
35836
MIR-129-5P 10
MI0000448 , MI0000156 , MI0000903 , MI0002525 , MI0002524 , MI0002526 , MI0002527 MIMAT0000836 , MIMAT0000141 , MIMAT0000425 , MIMAT0002236 , MIMAT0002235 , MIMAT0002237 , MIMAT0002238 MIR-130A
35837
MIR-130A 11
MI0000904 , MI0000748 , MI0000408 MIMAT0000837 , MIMAT0000387 , MIMAT0000691 MIR-130B
35838
MIR-130B 12
MI0000474 , MI0000160 , MI0000907 , MI0002589 , MI0002591 , MI0002592 , MI0002590 MIMAT0000840 , MIMAT0000447 , MIMAT0000146 , MIMAT0002288 , MIMAT0002290 , MIMAT0002291 , MIMAT0002289 MIR-134
35841
MIR-134 13
MI0000475 , MI0000162 , MI0000909 , MI0002594 , MI0001523 , MI0002596 , MI0002593 , MI0002595 , MI0002452 MIMAT0000148 , MIMAT0000448 , MIMAT0000842 , MIMAT0002293 , MIMAT0001417 , MIMAT0002295 , MIMAT0002292 , MIMAT0002294 , MIMAT0002158 MIR-136
35843
MIR-136 14
MI0000454 , MI0000163 , MI0000910 , MI0005299 , MI0001221 MIMAT0000843 , MIMAT0000429 , MIMAT0000149 , MIMAT0004110 , MIMAT0001153 MIR-137
35844
MIR-137 15
MI0000456 , MI0000611 , MI0000165 , MI0002547 , MI0002437 , MI0001229 MIMAT0000573 , MIMAT0000431 , MIMAT0000151 , MIMAT0002255 , MIMAT0002143 , MIMAT0001159 MIR-140-5P
35847
MIR-140-5P 16
MI0000457 , MI0000166 , MI0000914 , MI0002487 , MI0002485 , MI0002489 MIMAT0000846 , MIMAT0000153 , MIMAT0000432 , MIMAT0002198 , MIMAT0002196 , MIMAT0002200 MIR-141
35849
MIR-141 17
MI0000917 , MI0000460 , MI0000168 , MI0002556 , MI0002557 , MI0002554 , MI0002555 MIMAT0000156 , MIMAT0000436 , MIMAT0000850 , MIMAT0002264 , MIMAT0002265 , MIMAT0002262 , MIMAT0002263 MIR-144
35854
MIR-144 18
MI0000461 , MI0000169 , MI0000918 , MI0004756 , MI0002560 , MI0002558 , MI0002562 , MI0005305 , MI0002559 , MI0002561 , MI0002417 MIMAT0000851 , MIMAT0000157 , MIMAT0000437 , MIMAT0003542 , MIMAT0002268 , MIMAT0002266 , MIMAT0002270 , MIMAT0004116 , MIMAT0002267 , MIMAT0002269 , MIMAT0002123 MIR-145
35855
MIR-145 19
MI0000477 , MI0000170 , MI0000919 , MI0001235 MIMAT0000852 , MIMAT0000158 , MIMAT0000449 , MIMAT0001163 MIR-146A
35856
MIR-146A 20
MI0000253 , MI0000550 , MI0004737 , MI0002418 , MI0001189 MIMAT0000516 , MIMAT0000243 , MIMAT0003522 , MIMAT0002124 , MIMAT0001120 MIR-148A
35857
MIR-148A
1607 129 Kbytes .Supplementary data files not provided
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