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Platform GPL17653 Query DataSets for GPL17653
Status Public on Aug 30, 2013
Title PIQOR (TM) Immunology Microarray, human antisense
Technology type spotted DNA/cDNA
Distribution commercial
Organism Homo sapiens
Manufacturer Miltenyi Biotec GmbH, Cologne, Germany
Manufacture protocol Defined 200-400 bp fragments of selected cDNAs were generated by RT-PCR, cloned and sequence-verified. Amplified and purified cDNA inserts were spotted in quadruplicate on PIQOR slides using a proprietary piezoelectric spotter. The PIQOR slides were coated in a 2 step process generating a surface which covalently binds DNA molecules.
 
Description The PIQOR (TM) Immunology Microarray is composed of 1076 human specific cDNAs. These cDNAs include for each array six house-keeping genes as positive controls. In addition four E. coli DNA fragments and the corresponding in vitro transcribed RNAs (spiking controls) provide a control for the labeling and hybridisation efficiency. Two negative controls are given by spotted herring sperm DNA and buffer, respectively. All features are spotted in quadruplicate. The Platform data table reflects a condensed representation of the quadruplicate features.
 
Web link http://www.miltenyibiotec.com
Contributor(s) Gerstmayer B, Bosio A
Submission date Aug 29, 2013
Last update date Sep 03, 2013
Contact name Silvia Rueberg
E-mail(s) silvia@miltenyibiotec.de
Organization name Miltenyi Biotec GmbH
Department Genomic Services
Street address Friedrich-Ebert-Str. 68
City Bergisch Gladbach
ZIP/Postal code 51429
Country Germany
 
Samples (29) GSM1225012, GSM1225013, GSM1225014, GSM1225015, GSM1225016, GSM1225017 
Series (1)
GSE50634 Gene expression profiles in individuals with asymptomatic infections against cognate household members with dengue fever

Data table header descriptions
ID
uniprot UniProt accession number
GB_LIST RefSeq accession numbers
ORF Name
miltenyi_id cDNA identifier at Miltenyi Biotec GmbH
SPOT_ID

Data table
ID uniprot GB_LIST ORF miltenyi_id SPOT_ID
1 P01584, Q96HE5, Q9UCT6, Q7RU01 NM_000576 IL1B 3
2 P01585, Q13169, P60568 NM_000586 IL2 5
3 P08700 NM_000588 IL3 7
4 P05113, Q13840 NM_000879 IL5 14
5 P13232 NM_000880 IL7 16
6 P10145, Q9C077, Q96RG6, Q6FGF6, Q6LAE6 NM_000584 IL8_HUMAN 18
7 P15248 NM_000590 IL9 20
8 P22301 NM_000572 IL10 22
9 P20809 NM_000641 IL11 24
10 P29459, Q96QZ1 NM_000882 IL12A 26
11 P29460 NM_002187 IL12B 28
12 P35225, O43644 NM_002188 IL13 30
13 P40222, Q8N2Y3, Q66K62, Q86T54, Q86T85, Q86T86, Q86Y86, Q86YW3 NM_175852 TXLN 32
14 P40933, Q93058, O43512, O00440, Q9UBA3 NM_000585, NM_172174 IL15_1 33
15 Q14005, Q16435, Q9UP18 NM_172217, NM_004513 IL16 35
16 Q16552 NM_002190 IL17A 37
17 Q14116, O75599 NM_001562 IL18 39
18 P01375, O43647, Q9P1Q2, Q9UIV3 NM_000594 TNF 41
19 P19438 NM_001065 TNFR1 43
20 P01579 NM_000619 IFNG 45

Total number of rows: 1074

Table truncated, full table size 57 Kbytes.




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Supplementary data files not provided
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