Dendron-modified glass slides (NSB27 NHS Slide) were preparedby using a standard (1 x 3) glass slide (Schott NEXTERION Glass B, ultrasonically cleaned, #1055132).). The slides were purchased (NSB POSTECH, Korea) and used for the phosphokinase antibody array. Antibodies in a printing buffer (0.01%BSA, 0.01% Tween 20, 0.5% glycerol in 1X PBS buffer (pH 8.5)) were spotted on the NHS-activated NSB27 slides by Genetix QArray-Mini microarray spotter. After the printing, the printed slides were incubated in a 70~85% humidified chamber for 5~12 hrs and then washed in 1XPBST (Sigma P3563-10PAK) and subsequently in 1X PBS (Sigma P4417-100TAB). Any chance of the printed slides getting dried in the process of washing was avoided.
Description
Dendron-coated phosphokinase antibody array (DPA) with 88 spots of mouse antibodies.
Age-dependent changes in phosphorylation of signaling molecules in brain tissues of Alzheimer's disease (AD) model mice
Data table header descriptions
ID
Position
Position on the array(Block, Column, Row)
NSB#
phosphosite identifier of Dendron-coated phosphokinase antibody array (DPA)
PT_LIST
Uniprot accession number of spotted antibodies
CST#
Accession number of spotted antibodies purchased from Cell Signaling Technology (http://www.cellsignal.com).
Protein name
The name of proteins which are captured by printed phospho-site specific antibodies
Phospho-site(Human, Mouse)
The location of phospho-site which are captured by printed phospho-site specific antibodies based on PhosphoSitePlus (www.phosphosite.org). S, T, Y means Serine, Threonine and Tyrosine.
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