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Platform GPL19411 Query DataSets for GPL19411
Status Public on Nov 14, 2014
Title Wilson human antibody microarray H3/H7
Technology type antibody
Distribution non-commercial
Organism Homo sapiens
Manufacturer Patrick Wilson's laboratory, University of Chicago using ArrayIt technology (SpotBot3 microarrayer)
Manufacture protocol 1. 6-8µl of each human monoclonal antibodies at 500µg/ml are deposited in a 384 wells plate (ArrayIt)
2. the same volume of 2X Printing Buffer (ArrayIt) is added in each well. Mix well.
3. antibodies are then printed, in triplicate, on SuperEpoxy treated glass slides (ArrayIt). The same set of antibody is spotted 24 times on the same slide allowing 24 samples to be applied.
4. After the run, slides are kept at 4 degres before use.
 
Description Human monoclonal antibodies spotted.
The mAbs have been isolated from different individuals. Some antibodies are from the same individual (i.e., the ones starting by the same 3 numbers, for example 011) but they have been cloned from different cells (we isolate single B cells by flow cytometry and clone the heavy and light chain to make the mAbs).
 
Submission date Nov 13, 2014
Last update date Nov 14, 2014
Contact name Patrick C Wilson
E-mail(s) wilsonp@uchicago.edu
Phone 17737029009
Organization name University of Chicago
Department Medicine, Rheumatology
Street address 924 E 57th street, Knapp building
City CHICAGO
State/province Illinois
ZIP/Postal code 60637
Country USA
 
Samples (9) GSM1544409, GSM1544410, GSM1544411, GSM1544412, GSM1544413, GSM1544414 
Series (1)
GSE63249 Identification of H3N2 specific antibodies cross-reacting with H7 strains

Data table header descriptions
ID name of the antibody as mentioned in the manuscript
SPOT_ID influenza strain that the antibody is specific to

Data table
ID SPOT_ID
ID Strain
037-10036 5A01 A/Perth/16/2009 H3N2
011-10069 2C01 A/Perth/16/2009 H3N2
011-10069 3E06 A/Perth/16/2009 H3N2
011-10069 3G01 A/Perth/16/2009 H3N2
011-10069 5C01 A/Perth/16/2009 H3N2
011-10069 5D01 A/Perth/16/2009 H3N2
011-10069 5G01 A/Perth/16/2009 H3N2
011-10069 5G04 A/Perth/16/2009 H3N2
019-10117 3A06 A/Perth/16/2009 H3N2
019-10117 3C06 A/Perth/16/2009 H3N2
019-10117 3F01 A/Perth/16/2009 H3N2
019-10117 3F02 A/Perth/16/2009 H3N2
019-10117 1B02 A/Perth/16/2009 H3N2
028-10134 4F03 A/Perth/16/2009 H3N2
008-10053 5E04 A/Perth/16/2009 H3N2
008-10053 5G01 A/Perth/16/2009 H3N2
008-10053 6C05 A/Perth/16/2009 H3N2
008-10053 1G05 A/Perth/16/2009 H3N2
017-10117 5B03 A/Perth/16/2009 H3N2

Total number of rows: 84

Table truncated, full table size 2 Kbytes.




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