GEO help: Mouse over screen elements for information.
Status
Public on Oct 12, 2006
Title
MBPL Human 30k P4
Technology type
spotted DNA/cDNA
Distribution
non-commercial
Organism
Homo sapiens Manufacturer
MBPL in house arrayer
Manufacture protocol
Arrayer obtained from Dr. Patrick O. Brown MGuide II and ArrayMarker program version 2.3.2 (Dr. Joseph DeRisi, university of California, San Francisco) with 48 spotting pins (TeleChem).We control printing condition in temperature 25~27°C and humidity about 41~43%, and printing in June 2003.cDNA clones were purchased from Incyte Genomics and 7334 sequence-verified clones were amplified by PCR.We purified these 7334 clones by isopropanol precipitation and used 3×saline-sodium citrate (SSC) as spotting solution. cDNA spotted on GAPS Ⅱ coated slides (Corning Incorporated)coating of pure gamma amino propylsilane.
Support
glass
Coating
other
Description
There are 32448 spots within a array.7334 human clone were printing 4 duplicate in a array. Additionally, we printing 10 doping control genes (SpotReport?-10 Array Validation System , Stratagene) and one house-keeping gene , 5 negative control gene as quality control indicator. total 16 quality control indicator were printing 2 duplicate in each block.
Web link
http://mbpl.ns.nthu.edu.tw/chinese/chinese.htm Contributor(s)
Hsu IC , Tsai M , Lin C , Cheng W , Huang C , Chang C , Hsieh Y , Chang K , Weng T , Chang C Submission date
Jun 25, 2005
Last update date
Oct 12, 2006
Contact name
ChengWei Chang
E-mail(s)
d924546@oz.nthu.edu.tw Organization name
nthu
Department
bmes
Lab
mbpl
Street address
101, Section 2 Kuang Fu Road
City
Hsinchu
ZIP/Postal code
30013
Country
Taiwan
Samples (48)
GSM77003 , GSM77004 , GSM77005 , GSM77006 , GSM77085 , GSM77149
GSM77153 ,
GSM77156 ,
GSM77160 ,
GSM77162 ,
GSM77166 ,
GSM83154 ,
GSM83155 ,
GSM83156 ,
GSM83157 ,
GSM83158 ,
GSM83159 ,
GSM83160 ,
GSM83886 ,
GSM83887 ,
GSM83888 ,
GSM84191 ,
GSM84192 ,
GSM84193 ,
GSM84194 ,
GSM84195 ,
GSM84196 ,
GSM84197 ,
GSM84207 ,
GSM85347 ,
GSM182440 ,
GSM182441 ,
GSM182442 ,
GSM182443 ,
GSM182444 ,
GSM182445 ,
GSM182446 ,
GSM182447 ,
GSM182448 ,
GSM182449 ,
GSM1014585 ,
GSM1014587 ,
GSM1014590 ,
GSM1014593 ,
GSM1014599 ,
GSM1014601 ,
GSM1014605 ,
GSM1014606 Series (5)
GSE3421 cDNA microarray analysis of human keratinocytes irradiated by ELF-EMF
GSE3623 Transcriptional Activation of Mitochondria Respiratory Chain by UV irradiation
GSE3692 The Study of Gene Expression Profiles of KG-1 Cells Treated with Antrodia Camphorata Extracts using cDNA microarray.
GSE7589 UVB-induced persistent activation of cellular pathways revealed by a loop-designed microarray approach
GSE41319 Study of UVB responses of SV40 transformed human lung fibroblast: Control vs. UVB irradiated
Data table header descriptions ID BLOCK subarray (4 X 12 matix, up to 48)
COLUMN column within block(up to 26)
ROW row within block(up to 26)
SERIAL NUMBER Assignment of a number from 1 to 7334 to each cDNA clone
GB_ACC genebank accession number
SPOT_ID UNIGENE unigen accession number
ORGANISM source of organism
NAME full name of genes
SYMBOL symbol of genes
Data table ID BLOCK COLUMN ROW SERIAL NUMBER GB_ACC SPOT_ID UNIGENE ORGANISM NAME SYMBOL 1
1
1
1
100001
X56062 Arabidopsis thaliana
doping control
NULL
2
1
2
1
100003
U91966 Arabidopsis thaliana
doping control
NULL
3
1
3
1
100005
AF159803 Arabidopsis thaliana
doping control
NULL
4
1
4
1
100007
AF168390 Arabidopsis thaliana
doping control
NULL
5
1
5
1
100009
AF247559 Arabidopsis thaliana
doping control
NULL
6
1
6
1
100011
house-keeping gene Beta-actin
Homo Sapiens
house-keeping gene
Beta-actin
7
1
7
1
100013
NULL
NULL
NULL
HAT4
8
1
8
1
100015
NULL
NULL
NULL
ATPS
9
1
9
1
100016
NULL
NULL
NULL
ASA1
10
1
10
1
100014
NULL
NULL
NULL
HAT22
11
1
11
1
100012
NULL
NULL
NULL
GA4
12
1
12
1
100010
X58149 Arabidopsis thaliana
doping control
NULL
13
1
13
1
100008
AF198054 Arabidopsis thaliana
doping control
NULL
14
1
14
1
100006
AF191028 Arabidopsis thaliana
doping control
NULL
15
1
15
1
100004
AF159801 Arabidopsis thaliana
doping control
NULL
16
1
16
1
100002
X14212 Arabidopsis thaliana
doping control
NULL
17
1
17
1
4
H27491.1 460988
Homo Sapiens
Core-binding factor, beta subunit
CBFB
18
1
18
1
36
AA292388.1 535961
Homo Sapiens
Topoisomerase I binding, arginine/serine-rich
TOPORS
19
1
19
1
8
T29883.1 377894
Homo Sapiens
Grancalcin, EF-hand calcium binding protein
GCA
20
1
20
1
40
H82727.1 236774
Homo Sapiens
High mobility group nucleosomal binding domain 4
HMGN4
32448 2804 Kbytes .Supplementary data files not provided
Less...
More...