Fraunhofer Institute for Biomedical Research (Potsdam, Germany)
Manufacture protocol
1. Oligos were arrayed in 384 well plates with a final concentration of 10 µM in 3× SSC/0.01% SDS in a total volume of 25 µl. 2. Arrays were printed onto (3-Glycidyloxypropyl)trimethoxysilane (Sigma) activated glas slides using a contact-spotter (QArray 2, Moleculare Devices). 3. After spotting arrays were incubated overnight at 4°C in a humidified atmosphere and subsequently dried for 30 min at 30°C. 4. Arrays were stored in a dry atmosphere at room temperature until use.
Not4 and Not5 modulate translation elongation by Rps7A ubiquitination, Rli1 moonlighting, and condensates that exclude eIF5A
Data table header descriptions
ID
Anticodon
Identity of detected tRNAs based on their anticodons and amino acid they bear
Codon
Codons read by the detected tRNAs
SEQUENCE
Full length probe sequences (5' to 3') listed in the first sheet of the file; K=G/T, M=A/C, R=G/A, S=G/C, W=A/T, Y=T/C, B=G/T/C, D=G/A/T, H=A/T/C, V=G/A/C, N=G/A/T/C
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