NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Platform GPL28697 Query DataSets for GPL28697
Status Public on Dec 27, 2020
Title Microarrays of Bacillus encoded tRNAs
Technology type spotted oligonucleotide
Distribution non-commercial
Organism Bacillus subtilis
Manufacturer Fraunhofer Institute for Biomedical Research (Potsdam, Germany)
Manufacture protocol 1. Oligos were arrayed in 384 well plates with a final concentration of 10 µM in 3× SSC/0.01% SDS in a total volume of 25 µl.
2. Arrays were printed onto (3-Glycidyloxypropyl)trimethoxysilane (Sigma) activated glas slides using a contact-spotter (QArray 2, Moleculare Devices).
3. After spotting arrays were incubated overnight at 4°C in a humidified atmosphere and subsequently dried for 30 min at 30°C.
4. Arrays were stored in a dry atmosphere at room temperature until use.
 
 
Submission date Jun 16, 2020
Last update date Jun 16, 2020
Contact name Zoya Ignatova
E-mail(s) zoya.ignatova@chemie.uni-hamburg.de
Organization name Universitaet Hamburg
Street address Martin -Luther-King-Platz 6
City Hamburg
ZIP/Postal code 20146
Country Germany
 
Samples (3) GSM4618504, GSM4618505, GSM4618506
Series (1)
GSE152592 Structural Basis for Bacterial Ribosome-Associated Quality Control by RqcH and RqcP

Data table header descriptions
ID
Codon Codons read by the detected tRNAs
Anticodon
SEQUENCE Probe sequences (5' to 3') are DNA oligonucleotides that cover each full-length tRNA.

Data table
ID Codon Anticodon SEQUENCE
BL_AlaGGC Ala-GCA/G/T Ala-IGC TGGAGCATAGCGGGCTCGAACCGCTGACCTCTACACTGCCAGTGTAGCGCTCTCCCAGCTGAGCTAATGCCCC
BL_AlaTGC Ala-GCC/T Ala-A/C/UGC TGGAGCCTAGCGGGATCGAACCGCTGACCTCCTGCGTGCAAAGCAGGCGCTCTCCCAGCTGAGCTAAGGCCCC
BL_ArgACG Arg-AGA Arg-ICG CGCGCCCGAGAGGAGTCGAACCCCTAACCTTTTGATCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGC
BL_ArgCCG Arg-AGG Arg-C/UCG CGCGCTCGGAGGGATTCGAACCCCCGGCAGACGTGGTACCGGAAACCACCGCTCTATCCAGCTGAGCTACGAGCGC
BL_ArgCCT Arg-CGC/T/A Arg-CCU CGTCCCAGGAGAGATTCGAACTCCCGACACACAGCTTAGGAGGCTGTTGCTCTATCCACCTGAGCTACTGGGAC
BL_ArgTCT Arg-CGG Arg-UCU TGACGTCCCAGGAGAGATTCGAACTCCCGACCGACGGCTTAGAAGGCCGTTGCTCTATCCAGCTGAGCTACTGGGAC
BL_AsnGTT Asn-AAC/T Asn-GUU CTCCRCAGGCAGGATTCGAACCTGCGACCGATCGGTTAACAGCCGATAGCTCTACCACTGAGCTACTGTGGA
BL_AspGTC Asp-GAC/T Asp-GUC GTCCGGACGGGACTCGAACCCGCGACCTCCTGCGTGACAGGCAGGCATTCTAACCAACTGAACTACCGGACC
BL_CysGCA Cys-TGC/T Cys-UGU/C AGGCGACACCCGGATTCGAACCGGGGATAAAGGTTTTGCAGACCTCTGCCTTACCACTTGGCTATGCCGCC
BL_GlnTTG Gln-CAA/G Gln-C/UUG CTGGGCTAGCTGGATTCGAACCAGCGCRTGASGGAGTCAAAGTCCGTTGCCTTACCGCTTGGCTATAGCCCA
BL_GluTTC Glu-GAA/G Glu-UUC TGACCCGTACGGGATTCGAACCCGTGTTACCGCCGTGAAAGGGCGGTGTCTTAACCACTTGACCAACGGGCC
BL_GlyGCC Gly-GGA/G/T Gly-G/CCC AGCGGAAGACGGGATTCGAACCCGCGACCCCCACCTTGGCAAGGTGGTGTTCTACCACTGAACTACTTCCGC
BL_GlyTCC Gly-GGC/T Gly-UCC AGCGGGTGATGRGAATCGAACYCACGACATCAGCTTGGAAGGCTGAGGTTTTACCACTAAACTACACCCGC
BL_HisGTG His-CAC/T His-GUG GGCGGTTGATGGGAATCGAACCCACGAATGAGAGCCACAATCTGGTGCGTTAACCACTTCGCCACAACCGC
BL_IleGAT Ile-ATC/T Ile-IAU TGGGCCTGAGTGGACTCGAACCAYCGACCTCACGCTTATCAGGCGTGCGCTCTAACCAGCTGAGCTACAGGCCCG
BL_LeuCAA Leu-CTA/G Leu-A/UAG TGATACCGGTGGTCGGGGTCGAACCGACACTCCCGAAGGAACACGATTTTGAGTCGTGCGCGTCTGCCAATTCCGCCACACCGGC
BL_LeuCAG Leu-CTC/T Leu-CAG TGCGGATGAAGGGACTTGAACCCCCACGTCTTTAAAAGACACTAGAGCCTGATTCTAGCGCGTCTGCCAATTCCGCCACATCCGC
BL_LeuGAG Leu-CTG Leu-CAA TGCGGCCGAGAGGACTTGAACCTCCACGGGTTATTCACCCACTAGGCCCTCAACCTAGCGCGTCTGCCATTCCGCCACGACCGC
BL_LeuTAA Leu-TTA/G Leu-UAA1 TGCCGAGGGCCGGACTTGAACCGGCACGGTAGTCACCTACCGCAGGATTTTAAGTCCTGTGTGTCTGCCAATTCCACCACCCCGGC
BL_LeuTAG Leu-TTG Leu-UAA2 TGCGGGTGAAGGGACTCGAACCCCCACGCCGTAAAGACACTAGATCCTAAGTCTAGCGCGTCTGCCAATTCCGCCACACCCGC

Total number of rows: 40

Table truncated, full table size 4 Kbytes.




Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp

Supplementary data files not provided
| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap