Microarrays containing these oligos are synthesized on glass with Expedite DNA synthesizers (Applied Biosystems, Foster City, CA) in conjunction with Maskless Array Synthesis units (NimbleGen Systems, Madison, WI).
Description
Oligo nucleotides 36mer in length are designed to represent sense and antisense strands of the nonrepetitive DNA of the indica rice genome. The probes were selected from the August 1, 2003 indica sequence (Annotated by Beijing Genomics Institue).
Chromosome from which the oligonucleotide sequence was selected
POSITION
Chromosome start position of the oligonucleotide relative to Beijing Genomics Institue rice assembly, Aug 1, 2003
STRAND
Sense or antisense strand of the chromosome sequence represented by the oligonucleotide probe
SEQUENCE
DNA sequence of the oligonucleotide
FREQUENCY
Oligomer frequency information calculated to determine the uniqueness of every 36mer subsequence in the genome. This measure was used to select probes that occur fewer than 5 times on average to reduce the potential for cross-hybridization.
