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Platform GPL30116 Query DataSets for GPL30116
Status Public on Aug 18, 2021
Title Microarrays of Bacillus encoded tRNAs [2020_bacillus.gal]
Technology type spotted oligonucleotide
Distribution non-commercial
Organism Enterococcus faecalis
Manufacturer Fraunhofer Institute for Biomedical Research (Potsdam, Germany)
Manufacture protocol 1. Oligos were arrayed in 384 well plates with a final concentration of 10 µM in 3× SSC/0.01% SDS in a total volume of 25 µl.
2. Arrays were printed onto (3-Glycidyloxypropyl)trimethoxysilane (Sigma) activated glas slides using a contact-spotter (QArray 2, Moleculare Devices).
3. After spotting arrays were incubated overnight at 4°C in a humidified atmosphere and subsequently dried for 30 min at 30°C.
4. Arrays were stored in a dry atmosphere at room temperature until use.
 
 
Submission date May 11, 2021
Last update date Jun 21, 2021
Contact name Zoya Ignatova
E-mail(s) zoya.ignatova@chemie.uni-hamburg.de
Organization name Universitaet Hamburg
Street address Martin -Luther-King-Platz 6
City Hamburg
ZIP/Postal code 20146
Country Germany
 
Samples (19) GSM5290167, GSM5290168, GSM5290169, GSM5290170, GSM5290171, GSM5290172 
Series (1)
GSE174254 RqcH and RqcP catalyze processive poly-alanine synthesis

Data table header descriptions
ID Identity of detected tRNAs based on their anticodons and amino acid they bear
Recognized Codon Codons read by the detected tRNAs
Corresponding Anticodon
Sequence Probe sequences (5' to 3') are DNA oligonucleotides that cover each full-length tRNA.

Data table
ID Recognized Codon Corresponding Anticodon Sequence
ArgACG Arg-CGC/T/A Arg-U/A/GCG CGCGCCCGAGAGGAGTCGAACCCCTAACCTTTTGATCCGTAGTCAAACGCTCTATCCAATTGAGCTACGGGCGC
ArgCCG Arg-CGG Arg-CCG CGCGCTCGGAGGGATTCGAACCCCCGGCAGACGTGGTACCGGAAACCACCGCTCTATCCAGCTGAGCTACGAGCGC
ArgCCT Arg-AGG Arg-CCU CGTCCCAGGAGAGATTCGAACTCCCGACACACAGCTTAGGAGGCTGTTGCTCTATCCACCTGAGCTACTGGGAC
ArgTCT Arg-AGA Arg-UCU TGACGTCCCAGGAGAGATTCGAACTCCCGACCGACGGCTTAGAAGGCCGTTGCTCTATCCAGCTGAGCTACTGGGAC
HisGTG His-CAC/T His-A/GUG GGCGGTTGATGGGAATCGAACCCACGAATGAGAGCCACAATCTGGTGCGTTAACCACTTCGCCACAACCGC
LysTTT Lys-AAA/G Lys-UUU TGAGCCATGAAGGACTCGAACCTTCGACCCTCTGATTAAAAGTCAGATGCTCTACCAACTGAGCTAATGGCTC
AspGTC Asp-GAC/T Asp-A/GUC GTCCGGACGGGACTCGAACCCGCGACCTCCTGCGTGACAGGCAGGCATTCTAACCAACTGAACTACCGGACC
GluTTC Glu-GAA/G Glu-C/UUC TGACCCGTACGGGATTCGAACCCGTGTTACCGCCGTGAAAGGGCGGTGTCTTAACCACTTGACCAACGGGCC
AsnGTT Asn-AAC/T Asn-A/GUU CTCCRCAGGCAGGATTCGAACCTGCGACCGATCGGTTAACAGCCGATAGCTCTACCACTGAGCTACTGTGGA
CysGCA Cys-TGC/T Cys-A/GCA AGGCGACACCCGGATTCGAACCGGGGATAAAGGTTTTGCAGACCTCTGCCTTACCACTTGGCTATGCCGCC
GlnTTG Gln-CAA/G Gln-C/UUG CTGGGCTAGCTGGATTCGAACCAGCGCRTGASGGAGTCAAAGTCCGTTGCCTTACCGCTTGGCTATAGCCCA
SerGCT Ser-AGC/T Ser-A/GCU CGGAGAAGGAGGGATTTGAACCCTCGCGCCGCTTACGCGACCTACACCCTTAGCAGGGGCGCCTCTTCAGCCACTTGAGTACTTCTCC
SerGGA Ser-TCC/T Ser-A/GGA CGGAGAGCAAGGGATTCGAACCCTTGAGACGGCGTTGGCCGCCTACACGATTTCCAATCGTGCTCCTTCGACCACTCGGACAGCTCTCC
SerTGA Ser-TCA/G/T Ser-A/C/UGA CGGAGGAAGAGGGATTCGAACCCCCGCGGGCTTTGACACCCCTGTCGGTTTTCAAGACCGATCCCTTCAGCCAGACTTGGGTATTCCTCC
ThrGGT Thr-ACC/T Thr-A/GGU AGCTTCCAAGCGGGCTCGAACCGCTGACCTCTTCCTTACCATGGAAGTGCTCTACCTGCTGAGCTATGGAAGC
ThrTGT Thr-ACA/G/T Thr-A/C/UGU TGCCGGCCAGAGGACTTGAACCCCCAACCTACTGATTACAAGTCAGTTGCTCTACCAATTGAGCTAGACCGGC
ThrYGT Thr-ACG Thr-CGU TGCCGGCAAGAGGACTTGAACCCCCAACCTACTGATTACGATTCAGTTGCTCTACCAATTGAGCTACACCGGC
AlaGGC Ala-GCC/T Ala-A/GGC TGGAGCATAGCGGGCTCGAACCGCTGACCTCTACACTGCCAGTGTAGCGCTCTCCCAGCTGAGCTAATGCCCC
AlaTGC Ala-GCA/G/T Ala-A/C/UGC TGGAGCCTAGCGGGATCGAACCGCTGACCTCCTGCGTGCAAAGCAGGCGCTCTCCCAGCTGAGCTAAGGCCCC
GlyGCC Gly-GGC/T Gly-A/GCC AGCGGAAGACGGGATTCGAACCCGCGACCCCCACCTTGGCAAGGTGGTGTTCTACCACTGAACTACTTCCGC

Total number of rows: 40

Table truncated, full table size 4 Kbytes.




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