|
| Status |
Public on Apr 11, 2023 |
| Title |
Neisseria meningitidis Protein Microarray (OMV) |
| Technology type |
spotted peptide or protein |
| Distribution |
non-commercial |
| Organism |
Neisseria meningitidis |
| Manufacturer |
Molecular Biology and Bacteriology lab, GSK, Siena |
| Manufacture protocol |
Purified recombinant proteins were diluted at final concentration of 0.5 mg/ml in 40% glycerol. Recombinant E. coli GMMA and OMV from NZ98/254 were prepared at 1 mg/ml in 20% glycerol and then serially diluted in 20% glycerol (step-2 dilutions). Samples were transferred into 384-well plates. Two droplets of 0.1 nL protein solution was deposited per spot on nitrocellulose-coated slides (FAST slides, Maine Manufacturing) using the non-contact printer Marathon Argus Spotter (Arrayjet). Printing was performed in a cabinet with controlled humidity and temperature (50-55% and 18°C, respectively). Each protein was printed in replicates on each slide. Printed protein microarrays were kept at room temperature for 5 hours, dried under a stream of nitrogen and store at 4°C. Only the highest concentration of the vesicles was included in the final dataset.
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| |
|
| Description |
spotted microarray containing OMV (E.coli outer membrane vesicles)
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| |
|
| Submission date |
Feb 02, 2023 |
| Last update date |
Apr 13, 2023 |
| Contact name |
Erika Bartolini |
| Organization name |
GSK Vaccines
|
| Department |
AIMB
|
| Lab |
Molecular Biology
|
| Street address |
Via Fiorentina 1
|
| City |
Siena |
| State/province |
Italy |
| ZIP/Postal code |
53100 |
| Country |
Italy |
| |
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| Samples (4) |
GSM7020126, GSM7020127, GSM7020128, GSM7020129 |
| Series (2) |
| GSE224291 |
ARTICLE OPEN OpcA and PorB are novel bactericidal antigens of the 4CMenB vaccine in mice and humans |
| GSE224348 |
OpcA and PorB are novel bactericidal antigens of the 4CMenB vaccine in mice and humans |
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