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Platform GPL3466 Query DataSets for GPL3466
Status Public on May 17, 2006
Title NCI Mus musculus 10K FCRF UniGEM2
Technology type spotted DNA/cDNA
Distribution non-commercial
Organism Mus musculus
Manufacturer NCI-Frederick/LMT Microarray Research Facility
Manufacture protocol The custom arrays are manufactured by the NCI Frederick Laboratory of Molecular Technology Microarray Facility (NIH/NCI/SAIC/LMT). Microarrays are printed with BioRobotics TAS II robotic arrayer from Genomic Solutions that is fitted with a printhead containing 32 SMP3 printing pins from Telechem International. The mouse cDNA probe set is based on 9984 PCR products from Incyte's mouse UniGEM 2.0 library. The set includes 9234 Unique GenBank Accessions; 8882 mapped into Mm UniGene Build #1201; 8656 Unique UniGene Clusters; 3463 EST clusters; 5193 Named clusters. The cDNA probes are distributed into 384-well polypropylene plates (Genetix, 384 micro array) and dissolved in 3XSSC print buffer at a concentration of 50-100 ug/ml. The microarray spot densities are 235 microns (center-to-center), and one spot is printed per probe per slide. The microarrays are printed on Fisher Premium microscope slides (cat# 12-544-1) coated with poly-L-lysine and post-processed according to the procedures on the mAdb NCI website.
Support glass
Coating polysine
 
 
Submission date Feb 17, 2006
Last update date Jan 17, 2013
Contact name Paolo Fardin
E-mail(s) paolofardin@ospedale-gaslini.ge.it
Organization name Istiuto Giannina Gaslini
Department Molecular Biology
Street address Largo G. Gaslini 5
City Genoa
ZIP/Postal code 16147
Country Italy
 
Samples (2) GSM97184, GSM97185
Series (1)
GSE4274 ANA1 picolinic acid

Data table header descriptions
ID
MADB_WELL_ID NIH mAdb plate-well identifier
CLONE_ID Clone identifier
GENE Gene Name
UNIGENE UniGene identifier
DESCRIPTION Gene description
SPOT_ID Alternative spot identifier

Data table
ID MADB_WELL_ID CLONE_ID GENE UNIGENE DESCRIPTION SPOT_ID
641311_1 641311 IMAGE:313176 Ppara 212789 Peroxisome proliferator activated receptor alpha
641312_1 641312 IMAGE:539908 Mrpl27 34951 RIKEN cDNA A630055F16 gene
641313_1 641313 IMAGE:313156 Unknown
641314_1 641314 IMAGE:540258 Arts1 83526 Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator
641315_1 641315 IMAGE:314211 AW551984 208855 Expressed sequence AW551984
641316_1 641316 IMAGE:540466 Ndnl2 19944 Necdin-like 2
641317_1 641317 IMAGE:315069 E130106K10Rik 227117 RIKEN cDNA 130106K10 gene
641318_1 641318 IMAGE:540673 Phf6 26870 PHD finger protein 6
641319_1 641319 IMAGE:315392 2500002K03Rik 29181 RIKEN cDNA 2500002K03 gene
641320_1 641320 IMAGE:539514 BC058674 219137 CDNA sequence BC058674
641321_1 641321 IMAGE:314814 B430006D22Rik 211030 CDNA sequence BC037432
641322_1 641322 IMAGE:539758 Uchl5 261004 Ubiquitin carboxyl-terminal esterase L5
641323_1 641323 IMAGE:317393 Notch4 173813 Notch gene homolog 4 (Drosophila)
641324_1 641324 IMAGE:539104 Rhoe 46497 Ras homolog gene family, member E
641325_1 641325 IMAGE:438498 Dtnb 286202 Dystrobrevin, beta
641326_1 641326 IMAGE:539212 Ppil2 253614 Peptidylprolyl isomerase (cyclophilin)-like 2
641327_1 641327 IMAGE:427435 Cav3 3924 Caveolin 3
641328_1 641328 IMAGE:539308 Unknown
641329_1 641329 IMAGE:438308 Ap3s2 220173 Adaptor-related protein complex 3, sigma 2 subunit
641330_1 641330 IMAGE:538698 Zfp143 10815 Zinc finger protein 143

Total number of rows: 9984

Table truncated, full table size 728 Kbytes.




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Supplementary data files not provided
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