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| Status |
Public on Aug 22, 2024 |
| Title |
IFC UNAM Mouse 22K v1 |
| Technology type |
spotted oligonucleotide |
| Distribution |
non-commercial |
| Organism |
Mus musculus |
| Manufacturer |
Unidad de Microarreglos, IFC UNAM, México |
| Manufacture protocol |
1. The Mouse 65-mer oligo library from Sigma-Genosys oligo sets was used. It contains 22,000 gene specific oligonucleotide probes representing the 70% of mouse genome.
2. Oligo library was resuspended to 50µM in Micro Spotting solution (ArrayIt Brand Products.).
3. SuperAmine coated slides 25x75 mm (TeleChem International INC) were printed using SDDC robot (Virtek) with 48 pins (Y= 12 meta rows; X= 4 meta columns; Y1= 22 rows; X1= 22 columns; with a dot spacing of Y1= 190 micron and X1= 190 microns). Fixed at 80°C for 4 hours.
4. For pre-hybridization the slides were re-hydrated with water vapor at 60°C, and fixed with two cycles of UV light (1200J). After boiling for two minutes at 92°C, slides were washed with 95% ethanol for one minute and prehybridzed in 5X SSC, 0.1% SDS and 1% BSA for one hour at 42°C.
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| Submission date |
Aug 16, 2024 |
| Last update date |
Aug 22, 2024 |
| Contact name |
Ana Soledad Sandoval-Rodriguez |
| E-mail(s) |
anasol44@gmail.com
|
| Phone |
+52-3339526314
|
| Organization name |
University of Guadalajara
|
| Department |
Molecular Biology and Genomics
|
| Lab |
Institute of Molecular Biology in Medicina and Gene Therapy
|
| Street address |
Sierra Mojada 950
|
| City |
Guadalajara |
| State/province |
Jalisco |
| ZIP/Postal code |
44340 |
| Country |
Mexico |
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| Samples (3) |
GSM8465144, GSM8465145, GSM8465146 |
| Series (1) |
| GSE275069 |
Mice livers: high fat diet versus high fat diet (HF) supplemented with pirfenidone (HFP) |
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