|
| Status |
Public on Mar 28, 2006 |
| Title |
Eurogentec S. pombe ORF array (D040E) |
| Technology type |
spotted DNA/cDNA |
| Distribution |
custom-commercial |
| Organism |
Schizosaccharomyces pombe |
| Manufacturer |
Eurogentec AG |
| Manufacture protocol |
Primary PCR: The PCR reactions are performed using tagged genome specific primers in the presence of genomic DNA (provided by your laboratory). We normally achieve a success rate of 93 % of PCR reactions. For the failed PCR reactions we perform three different protocols of PCR conditions, which include different MgCl2 concentrations and different Taq polymerase. We should normally get an additional 2 % working and reach a success rate of 95 % in total. We check 5 % of the total oligos by Mass Spectrometry and will re-synthesize maximum 2 % of the oligos based on a newly designed primer. We will perform the PCR reactions with the new designed oligonucleotides with the standard PCR protocol. Following this process we reach usually 97 % success rate.
|
| Support |
glass |
| Coating |
unknown |
| |
|
| Description |
11520 ORFs were spotted in duplicate on glass slide
|
| |
|
| Submission date |
Mar 25, 2006 |
| Last update date |
Jan 18, 2013 |
| Contact name |
Claes M Gustafsson |
| E-mail(s) |
claes.gustafsson@medkem.gu.se
|
| Organization name |
Gothenburg University
|
| Department |
Department of Medical biochemistry and cell biology
|
| Street address |
Medcinaregatan 9A
|
| City |
Gothenburg |
| ZIP/Postal code |
405 30 |
| Country |
Sweden |
| |
|
| Samples (4) |
GSM102843, GSM102848, GSM102850, GSM102851 |
| Series (1) |
| GSE3213 |
Genome-wide occupancy profile of mediator and the srb8-11 module reveals interactions with coding regions. |
|
