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Platform GPL3719 Query DataSets for GPL3719
Status Public on May 01, 2007
Title Yang et. al. Oligoarray of CfMNPV select ORFs
Technology type spotted oligonucleotide
Distribution non-commercial
Organism Choristoneura fumiferana multiple nucleopolyhedrovirus
Manufacturer Biotechnology Research Institute, Montreal, Canada
Manufacture protocol Spotted 70 mer nucleotides on glass
Support glass
Coating aminosilane
 
Description The array included 46 oligonucleotides (1.0 µg/µl) representing 23 CfMNPV ORFs, one for the coding strand and the other for the non-coding strand. Purified viral genomic DNA (50, 25, 5 ng/µl) was used as a positive control. A mixture of 18 viral oligonucleotides (50 ng/µl each) was served as a second positive control. The array included 2 oligos (At1 and At2) of Arabidopsis chlorophyll synthase gene to be hybridized with the spike RNA, and 2 oligos (HAT4, YaaJ) as negative controls. The array also included 5 insect internal control oligos. Each probe was printed in triplicate.
 
Contributor(s) Yang D, Barari M, Krell PJ
Submission date May 02, 2006
Last update date Apr 01, 2008
Contact name Dan-Hui Yang
E-mail(s) dyang@uoguelph.ca
Phone 1-519-8244120 Ext.53264
Fax 1-519-8371802
URL http://www.uoguelph.ca/mcb
Organization name University of Guelph
Department Dept. of Molecular and Cellular Biology
Lab Dr. Krell's lab
Street address 488 Gordon Street
City Guelph
State/province Ontario
ZIP/Postal code N1G 2W1
Country Canada
 
Samples (14) GSM107978, GSM108049, GSM108050, GSM108053, GSM108056, GSM108057 
Series (1)
GSE4783 Transcriptional analysis of select CfMNPV genes and some antisense transcripts by an oligonucleotide microarray

Data table header descriptions
ID
OLIGO_ID oligonucleotide name in the array
Block array
Column column in the array
Row row in the array
GI GenBank identifier
SEQUENCE oligonucleotide sequence
Organism
SPOT_ID
PT_GI

Data table
ID OLIGO_ID Block Column Row GI SEQUENCE Organism SPOT_ID PT_GI
1 11 1 1 1 57903534 CGCACGGTCACATCAAGGAAGAAGCCAAGCGTTCAGAAACAATCTACAGCTAGTAATTAACAATGACCTCGCGCACGGTT Choristoneura fumiferana nucleopolyhedrovirus
2 11 1 2 1 57903534 CGCACGGTCACATCAAGGAAGAAGCCAAGCGTTCAGAAACAATCTACAGCTAGTAATTAACAATGACCTCGCGCACGGTT Choristoneura fumiferana nucleopolyhedrovirus
3 11 1 3 1 57903534 CGCACGGTCACATCAAGGAAGAAGCCAAGCGTTCAGAAACAATCTACAGCTAGTAATTAACAATGACCTCGCGCACGGTT Choristoneura fumiferana nucleopolyhedrovirus
4 11-nc 1 4 1 57903534 AACCGTGCGCGAGGTCATTGTTAATTACTAGCTGTAGATTGTTTCTGAACGCTTGGCTTCTTCCTTGATGTGACCGTGCG Choristoneura fumiferana nucleopolyhedrovirus
5 11-nc 1 5 1 57903534 AACCGTGCGCGAGGTCATTGTTAATTACTAGCTGTAGATTGTTTCTGAACGCTTGGCTTCTTCCTTGATGTGACCGTGCG Choristoneura fumiferana nucleopolyhedrovirus
6 11-nc 1 6 1 57903534 AACCGTGCGCGAGGTCATTGTTAATTACTAGCTGTAGATTGTTTCTGAACGCTTGGCTTCTTCCTTGATGTGACCGTGCG Choristoneura fumiferana nucleopolyhedrovirus
7 89 1 7 1 57903534 TATGTTTCACATGAGTTGGTTGATGCAAGTTGCTGATGTATTACCCGTCGTCGTTGTGCAAAACTCCTGAAAGCTTATTGA Choristoneura fumiferana nucleopolyhedrovirus
8 89 1 8 1 57903534 TATGTTTCACATGAGTTGGTTGATGCAAGTTGCTGATGTATTACCCGTCGTCGTTGTGCAAAACTCCTGAAAGCTTATTGA Choristoneura fumiferana nucleopolyhedrovirus
9 89 1 9 1 57903534 TATGTTTCACATGAGTTGGTTGATGCAAGTTGCTGATGTATTACCCGTCGTCGTTGTGCAAAACTCCTGAAAGCTTATTGA Choristoneura fumiferana nucleopolyhedrovirus
10 89-nc 1 10 1 57903534 TCAATAAGCTTTCAGGAGTTTTGCACAACGACGACGGGTAATACATCAGCAACTTGCATCAACCAACTCATGTGAAACATA Choristoneura fumiferana nucleopolyhedrovirus
11 89-nc 1 11 1 57903534 TCAATAAGCTTTCAGGAGTTTTGCACAACGACGACGGGTAATACATCAGCAACTTGCATCAACCAACTCATGTGAAACATA Choristoneura fumiferana nucleopolyhedrovirus
12 89-nc 1 12 1 57903534 TCAATAAGCTTTCAGGAGTTTTGCACAACGACGACGGGTAATACATCAGCAACTTGCATCAACCAACTCATGTGAAACATA Choristoneura fumiferana nucleopolyhedrovirus
13 slp 1 13 1 57903534 GGCGCAACCCGATTACGTGGAACGAGCTCGAGTACATTGGCGGTAACAACTCGGGACTAGTGCCTAACCC Choristoneura fumiferana nucleopolyhedrovirus
14 slp 1 14 1 57903534 GGCGCAACCCGATTACGTGGAACGAGCTCGAGTACATTGGCGGTAACAACTCGGGACTAGTGCCTAACCC Choristoneura fumiferana nucleopolyhedrovirus
15 slp 1 15 1 57903534 GGCGCAACCCGATTACGTGGAACGAGCTCGAGTACATTGGCGGTAACAACTCGGGACTAGTGCCTAACCC Choristoneura fumiferana nucleopolyhedrovirus
16 slp-nc 1 16 1 57903534 GGGTTAGGCACTAGTCCCGAGTTGTTACCGCCAATGTACTCGAGCTCGTTCCACGTAATCGGGTTGCGCC Choristoneura fumiferana nucleopolyhedrovirus
17 slp-nc 1 17 1 57903534 GGGTTAGGCACTAGTCCCGAGTTGTTACCGCCAATGTACTCGAGCTCGTTCCACGTAATCGGGTTGCGCC Choristoneura fumiferana nucleopolyhedrovirus
18 slp-nc 1 18 1 57903534 GGGTTAGGCACTAGTCCCGAGTTGTTACCGCCAATGTACTCGAGCTCGTTCCACGTAATCGGGTTGCGCC Choristoneura fumiferana nucleopolyhedrovirus
19 blank 1 19 1 --blank
20 blank 1 20 1 --blank

Total number of rows: 192

Table truncated, full table size 24 Kbytes.






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