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Status
Public on Jul 03, 2006
Title
Su(Ste) tiling and microRNA array (#4)
Technology type
in situ oligonucleotide
Distribution
custom-commercial
Organism
Drosophila melanogaster Manufacturer
LC Sciences (Houston, TX, USA)
Manufacture protocol
MicroRNA probes were designed by LC Sciences (based on Sanger Institute miRBase repository sequences on November 3, 2005). LNA-modified RNA probes were synthesized in situ using photogenerated acid coupled with conventional DMT chemistry (LNA-modification ensures Tm-normalization). Synthesis was performed on a µParaflo microfluidic chip.http://www.lcsciences.com for additionnal details.
Catalog number
UM_050496
Contributor(s)
Vagin VV , Sigova A , Li C , Seitz H , Gvozdev V , Zamore PD Submission date
May 25, 2006
Last update date
Jul 03, 2006
Contact name
Hervé Seitz
Phone
(508)856 1220
Fax
(508)856 2003
URL
http://www.umassmed.edu/bmp/faculty/zamore.cfm?start=0& Organization name
University of Massachusetts Medical School
Department
Biochemistry and Molecular Pharmacology
Lab
870 P, Lazare Research Building
Street address
364 Plantation Street
City
Worcester
State/province
MA
ZIP/Postal code
01605-2324
Country
USA
Samples (1)
GSM111136 Series (1)
GSE4932 A small RNA mechanism distinct from the RNAi and microRNA pathways silences selfish genetic elements in Drosophila.
Data table header descriptions ID Probe_ID Name of the detected RNA
SEQUENCE Sequence of the detected RNA
Row Position on array (row)
Column Position on array (column)
Position Position on array (single-cell format)
SPOT_ID
Data table ID Probe_ID SEQUENCE Row Column Position SPOT_ID 1
1
1
1-1:1
-- empty
2
dme-miR-1
UGGAAUGUAAAGAAGUAUGGAG
1
2
1-1:2
3
dme-miR-276b
UAGGAACUUAAUACCGUGCUCU
1
3
1-1:3
4
s-dme-bantam
CCAAAUCGAAAACCGGUUUGACU
1
4
1-1:4
5
s-dme-mir-263b
GGUUCUGCGGGUGCCAAAAC
1
5
1-1:5
6
snoRNA:U21
AUUGUGAUUAUUCAAGUUUAUUU
1
6
1-1:6
7
ANTISu(Ste 173
CCAAAGAUUUUCCCCGGCACGU
1
7
1-1:7
8
ANTISu(Ste 301
GCGUAAAUUUAAACGCACUUUA
1
8
1-1:8
9
ANTISu(Ste 429
UUAUAAUUUUGAUUCAAUCAUU
1
9
1-1:9
10
1
10
1-1:10
-- empty
11
ANTISu(Ste 557
UUUCCUGACUACAUCAAAUAUU
1
11
1-1:11
12
ANTISu(Ste 685
UCAUUCGAGUAGCAAAGCAUAC
1
12
1-1:12
13
ANTISu(Ste 813
CAGAAAUAUUUGUCCGAUAUAU
1
13
1-1:13
14
ANTISu(Ste 941
UCGCCCAAGUCCUCACCAAAGA
1
14
1-1:14
15
ANTISu(Ste 1069
GAGACCGCCCCUGGACAACCCA
1
15
1-1:15
16
ANTISu(Ste 1197
UGUGUGGGGCUAGUCAACGGUG
1
16
1-1:16
17
ANTISu(Ste 1325
CACGCCCGAUACAUCAGGUCGG
1
17
1-1:17
18
ANTISu(Ste 1453
UUUCAGGACACGUUCAGCCAGA
1
18
1-1:18
19
ANTISu(Ste 1581
UAUAGCAAGUCACAGUAAAAUC
1
19
1-1:19
20
Su(Ste) 58
CUUGCUAUAAUUUCUAUAGGAA
1
20
1-1:20
3968 226 Kbytes .Supplementary data files not provided
