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Status
Public on Jul 03, 2006
Title
roo tiling and microRNA array
Technology type
in situ oligonucleotide
Distribution
custom-commercial
Organism
Drosophila melanogaster Manufacturer
LC Sciences (Houston, TX, USA)
Manufacture protocol
MicroRNA probes were designed by LC Sciences (based on Sanger Institute miRBase repository sequences on December 7, 2005). LNA-modified RNA probes were synthesized in situ using photogenerated acid coupled with conventional DMT chemistry (LNA-modification ensures Tm-normalization). Synthesis was performed on a µParaflo microfluidic chip.http://www.lcsciences.com for additionnal details.
Catalog number
UM_050579
Contributor(s)
Vagin VV , Sigova A , Li C , Seitz H , Gvozdev V , Zamore PD Submission date
May 25, 2006
Last update date
Jul 03, 2006
Contact name
Hervé Seitz
Phone
(508)856 1220
Fax
(508)856 2003
URL
http://www.umassmed.edu/bmp/faculty/zamore.cfm?start=0& Organization name
University of Massachusetts Medical School
Department
Biochemistry and Molecular Pharmacology
Lab
870 P, Lazare Research Building
Street address
364 Plantation Street
City
Worcester
State/province
MA
ZIP/Postal code
01605-2324
Country
USA
Samples (1)
GSM111154 Series (1)
GSE4932 A small RNA mechanism distinct from the RNAi and microRNA pathways silences selfish genetic elements in Drosophila.
Data table header descriptions ID Probe_ID Name of the detected RNA
SEQUENCE Sequence of the detected RNA
Row Position on array (row)
Column Position on array (column)
Position Position on array (single-cell format)
SPOT_ID
Data table ID Probe_ID SEQUENCE Row Column Position SPOT_ID 1
1
1
1-1:1
-- empty
2
dme-miR-1
UGGAAUGUAAAGAAGUAUGGAG
1
2
1-1:2
3
dme-miR-79
UAAAGCUAGAUUACCAAAGCAU
1
3
1-1:3
4
s-dme-bantam
AGUCAAACCGGUUUUCGAUUUGG
1
4
1-1:4
5
s-dme-mir-263a
GAUCUCUUAGUGGCAUCUAUGG
1
5
1-1:5
6
snoRNA:U21
AUUGUGAUUAUUCAAGUUUAUUU
1
6
1-1:6
7
ANTIRoo 1
AUCUCAGCUUUGCUGGCCCUUUGGCAGAAU
1
7
1-1:7
8
ANTIRoo 641
CGGCAAGAACCGACGAUAAAAUUCUAGUUG
1
8
1-1:8
9
ANTIRoo 1281
AAUCACGGUAGGCUAAAUACAAAUAUUCUC
1
9
1-1:9
10
1
10
1-1:10
-- empty
11
ANTIRoo 1921
UAAAACCUCAGCAAUAUAUCUAGACCCGCU
1
11
1-1:11
12
ANTIRoo 2561
AUGAAGAAAUGUCAACUCUUUUAUGUCAAA
1
12
1-1:12
13
ANTIRoo 3201
AUAAAAAAUGCUCAUAAGGAAACAUUGCAU
1
13
1-1:13
14
ANTIRoo 3841
AUGGAUUAACAGUGGUCAAAGUAAGAUCAA
1
14
1-1:14
15
ANTIRoo 4481
ACAAUUCCAAAAUAUUAACCACUGUGGAGG
1
15
1-1:15
16
ANTIRoo 5121
AAUCUGGAGAAAAAACUAAAAAGAAAUGAA
1
16
1-1:16
17
ANTIRoo 5761
AUUGAUUGAUAGUGGCUCUCAAAGCACAAU
1
17
1-1:17
18
ANTIRoo 6401
UGGAUAUGCCAAAUUUAAAUUUAGAAUCCA
1
18
1-1:18
19
ANTIRoo 7041
UUAAACCUUGAAAUGCGUGGUCACCCUGAA
1
19
1-1:19
20
ANTIRoo 7681
GACUACAAAAAUAAUAAAACGUGCCAAAAA
1
20
1-1:20
3968 241 Kbytes .Supplementary data files not provided
