Agilent’s non-contact industrial inkjet printing process uniformly deposits oligo monomers onto specially-prepared glass slides. Custom microarrays are manufactured using Agilent’s non-contact in situ synthesis process of printing 60-mer length oligonucleotide probes, base-by-base, from digital sequence files. This is achieved with an inkjet process which delivers extremely small, accurate volumes (picoliters) of the chemicals to be spotted. Standard phosphoramidite chemistry used in the reactions allows for very high coupling efficiencies to be maintained at each step in the synthesis of the full-length oligonucleotide. Precise quantities are reproducibly deposited “on the fly.” This engineering feat is achieved without stopping to make contact with the slide surface and without introducing surface-contact feature anomalies, resulting in consistent spot uniformity and traceability.
Expression analysis and in silico characterization of intronic long noncoding RNAs in renal cell carcinoma: emerging functional associations (RCC expression)
Expression analysis and in silico characterization of intronic long noncoding RNAs in renal cell carcinoma: emerging functional associations
Data table header descriptions
ID
Spot Type
Genomic localization in protein-coding gene: Exonic (maps to a known exon), TIN (maps totally inside an intron), PIN (maps partially to an intron), Probe Type 1-4 (probes reserved for other studies), Agilent control (Agilent name of control)
Strand
Orientation of the sequence in comparison to the orientation of the protein-coding gene mapping to the same genomic locus: Sense (same direction as protein-coding gene), Antisense (opposite direction as protein-coding gene)
Locus Gene ID
The GeneID number from GenBank of a protein-coding gene mapping to the same genomic locus where the probe sequence maps
SEQUENCE
Sequence of probe (5' to 3')
SPOT_ID
Chromosome Map Location
Chromosome region of protein-coding gene (hg17 assembly, NCBI Build 35)
Less...
More...