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Platform GPL4486 Query DataSets for GPL4486
Status Public on Oct 31, 2006
Title Fox Chase Cancer Center Homo sapiens moNIA15k
Technology type spotted oligonucleotide
Distribution non-commercial
Organism Mus musculus
Manufacturer Fox Chase Cancer Center Microarray Facility
Manufacture protocol Targets:
cDNA (50-200ng/µl in 50% DMSO) 10µl
or
Oligo (20-100 µM in 50% DMSO) 10 µl
Targets are stored in a dessicator at –80 ºC after printing
Slides:
Home made Poly-L-Lysine coated slides should be aged for at least 3 weeks before printing. Coated slide can be stored at RT for at least 6 months.
Arrayer
OmniGrid (GeneMachines) with MicroSpotting pins (Telechem)
Printing Configuration
Pin 4 x 4
Spot-spot spacing 240 µm
Spot size 150-200 µm
Subgrid 18 x 18 spots
Grid 12 x 4
Humidity 45%
Temperature 20-25 ºC
Post-Processing of Arrays
Following printing, the slides are allowed to dry and spotted DNA is bound to slide by baking slides at 80 ºC for 2 hours. Then printed slides are blocked with succinic anhydride (Brown protocol).
Arrays are used immediately or stored in dessicator at RT for at least 6 months.
 
 
Contributor(s) Capo-chichi CD, Smedberg JL, Rula ME, Nicolas E, Yeung A, Adamo RF, Frolov A, Godwin AK, Xu X
Submission date Oct 25, 2006
Last update date Oct 31, 2006
Contact name Xiangxi Xu
E-mail(s) Xiangxi.Xu@fccc.edu
URL http://www.fccc.edu
Organization name Fox Chase Cancer Center
Department Ovarian Cancer and Tumor Cell Biology
Street address 333 Cottman Ave
City Philadelphia
State/province PA
ZIP/Postal code 19111
Country USA
 
Samples (4) GSM142368, GSM142371, GSM142373, GSM142375
Series (1)
GSE6133 Alteration of differentiation potentials by modulating GATA transcription factors in murine embryonic stem cells

Data table header descriptions
ID
GB_ACC Genbank accession number, where available
PROBE_ID Genbank or NIA identifier
ID_TYPE Indicator of identifier source for PROBE_ID
SPOT_ID
DESCRIPTION Genbank Locus, or other, decriptor where available

Data table
ID GB_ACC PROBE_ID ID_TYPE SPOT_ID DESCRIPTION
FCCC_moNIA15k-1 AA409587 BG063085 GenBank Accession Number H3002A02-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3002A02-3', MRNA sequence
FCCC_moNIA15k-2 AA409614 H3002A04 NIA Mouse cDNA Clone ID H3002A04 NIA Mouse 15K cDNA Clone Set (no GB accession # found)
FCCC_moNIA15k-3 AA409620 BG063088 GenBank Accession Number H3002A06-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3002A06-3', MRNA sequence
FCCC_moNIA15k-4 AA409627 BG063090 GenBank Accession Number H3002A08-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3002A08-3', MRNA sequence
FCCC_moNIA15k-5 AA409650 BG063092 GenBank Accession Number H3002A10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3002A10-3', MRNA sequence
FCCC_moNIA15k-6 AA409520 BG063094 GenBank Accession Number H3002A12-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3002A12-3', MRNA sequence
FCCC_moNIA15k-7 AA409782 BG062940 GenBank Accession Number H3002C02-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3002C02-3', MRNA sequence
FCCC_moNIA15k-8 AA409789 BG062942 GenBank Accession Number H3002C04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3002C04-3', MRNA sequence
FCCC_moNIA15k-9 AA409792 BG062943 GenBank Accession Number H3002C06-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3002C06-3', MRNA sequence
FCCC_moNIA15k-10 AA409744 BG062945 GenBank Accession Number H3002C08-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3002C08-3', MRNA sequence
FCCC_moNIA15k-11 AA409751 BG062946 GenBank Accession Number H3002C10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3002C10-3', MRNA sequence
FCCC_moNIA15k-12 AA409838 BG062948 GenBank Accession Number H3002C12-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3002C12-3', MRNA sequence
FCCC_moNIA15k-13 AA409980 BG062961 GenBank Accession Number H3002E02-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3002E02-3', MRNA sequence
FCCC_moNIA15k-14 AA409988 H3002E04 NIA Mouse cDNA Clone ID H3002E04 NIA Mouse 15K cDNA Clone Set (no GB accession # found)
FCCC_moNIA15k-15 AA410009 BG062963 GenBank Accession Number H3002E06-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3002E06-3', MRNA sequence
FCCC_moNIA15k-16 AA410017 H3002E08 NIA Mouse cDNA Clone ID H3002E08 NIA Mouse 15K cDNA Clone Set (no GB accession # found)
FCCC_moNIA15k-17 AA410155 BG062966 GenBank Accession Number H3002E10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3002E10-3', MRNA sequence
FCCC_moNIA15k-18 AA410169 BG062968 GenBank Accession Number H3002E12-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3002E12-3', MRNA sequence
FCCC_moNIA15k-19 AA409886 BG062981 GenBank Accession Number H3002G02-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3002G02-3', MRNA sequence
FCCC_moNIA15k-20 AA409995 BG062983 GenBank Accession Number H3002G04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3002G04-3', MRNA sequence

Total number of rows: 15552

Table truncated, full table size 2289 Kbytes.




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