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Platform GPL4700 Query DataSets for GPL4700
Status Public on May 14, 2007
Title OSU-CCC MicroRNA Microarray Version 2.0
Technology type spotted oligonucleotide
Distribution non-commercial
Organisms Homo sapiens; Mus musculus
Manufacturer Microarray Shared Resource, Comprehensive Cancer Center, The Ohio State University (OSU-CCC)
Manufacture protocol Manufacured using GE Healthcare's CodeLink (TM) 3-D Gel Matrix Technology. CodeLink(tm) Activated Slides are designed to covalently immoblize amine-modified DNA for microarrays. The slides are coated with a 3-D surface chemistry comprised of a long-chain, hydrophilic polymer containing amine-reactive groups. This polymer is covalently crosslinked to itself and to the surface of the slide. The crosslinked polymer, combined with end-point attachment, orients the immobilized DNA, and holds it away from the surface of the slide. The 1x printing buffer consists of 50 mM sodium phosphate at pH 8.5. Codelink slides are manufactured by SurModics and were formally sold as 3D-Link(tm) Activated Slides. ¹ Microarrays are printed with an Omnigrid robotic arrayer by Genomic Solutions that is fitted with a printhead and 16 SMP3 print tips from Telechem. The microRNA microarray probe set is based on 40mer oligonucleotides from Sigma-Genosys. ¹ Oligonucleotide probes are distributed into 384-well polypropylene plates (Genetix, 384 micro array) and dissolved in print buffer (150mM sodium phosphate, pH 8.5, 4% sodium sulfate, 0.0001% N-lauryl sarcosyl) to a normalized 25 micromolar concentration. The miRNA microarray spot densities are 200 microns (center-to-center). The microarrays are printed on Codelink Activated Slide (PN300011)) and treated by a method that is an adaptation of the manufacturer's instructions to allow higher throughput processing.
Support glass
Coating polyacrymide hydrogel polymer
Submission date Dec 26, 2006
Last update date May 14, 2007
Contact name Xin Wei Wang
Phone 240-760-6858
Organization name National Cancer Institute
Department Laboratory of Human Carcinogenesis
Lab Liver Carcinogenesis Unit
Street address 37 Convent Drive
City Bethesda
State/province MD
ZIP/Postal code 20892-4255
Country USA
Samples (709) GSM157887, GSM157888, GSM157889, GSM157890, GSM157891, GSM157892 
Series (3)
GSE6857 Unique MicroRNA Expression Signature Predicts Survival, Metastases and Recurrence of HCC
GSE7055 Expression of microRNAs and Protein-coding Genes Associated with Perineural Invasion in Prostate Cancer
GSE7828 MicroRNA profiles of 84 colon adenocarcinomas and paired nontumorous

Data table header descriptions
ID unique spot id
Slide_block Array block location
Slide_column Array column location
Slide_row Array row location
OLIGO_ID Oligo identifier
DESCRIPTION Description of content
MATURE_SANGER_REGISTRY_NAME Registry name in Sanger's mirBASE,

Data table
1-1-1 1 1 1 hsa-mir-033b-prec hsa-mir-033b-prec hsa-mir-033b mirn033b Homo sapiens
1-1-2 1 1 2 hsa-mir-125a-precNo2 hsa-mir-125a-precNo2 hsa-mir-125a mirn125a Homo sapiens
1-1-3 1 1 3 hsa-mir-153-1-prec1 hsa-mir-153-1-prec1 hsa-mir-153-1 mirn153-1 Homo sapiens
1-1-4 1 1 4 uc.124+ Control Control
1-1-5 1 1 5 uc.156+ Control Control
1-1-6 1 1 6 uc.6+A Control Control
1-1-7 1 1 7 uc.52+A Control Control
1-1-8 1 1 8 uc.84+A Control Control
1-1-9 1 1 9 uc.320+A Control Control
1-1-10 1 1 10 uc.364+A Control Control
1-1-11 1 1 11 mmu-mir-138-1No2 mmu-mir-138-1No2 mmu-mir-138-1 mirn138-1 Mus musculus
1-1-12 1 1 12 mmu-mir-96No2 mmu-mir-96No2 mmu-mir-96 mirn96 Mus musculus
1-1-13 1 1 13 hsa-mir-101-2No1 hsa-mir-101-2No1 hsa-mir-101-2 mirn101-2 Homo sapiens
1-1-14 1 1 14 hsa-mir-371No1 hsa-mir-371No1 hsa-mir-371 mirn371 Homo sapiens
1-1-15 1 1 15 Blank Blank Blank
1-1-16 1 1 16 Blank Blank Blank
1-1-17 1 1 17 Blank Blank Blank
1-1-18 1 1 18 Blank Blank Blank
1-2-1 1 2 1 hsa-mir-033b-prec hsa-mir-033b-prec hsa-mir-033b mirn033b Homo sapiens
1-2-2 1 2 2 hsa-mir-125a-precNo2 hsa-mir-125a-precNo2 hsa-mir-125a mirn125a Homo sapiens

Total number of rows: 11520

Table truncated, full table size 610 Kbytes.

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp

Supplementary data files not provided

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