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Status
Public on May 14, 2007
Title
OSU-CCC MicroRNA Microarray Version 2.0
Technology type
spotted oligonucleotide
Distribution
non-commercial
Organisms
Homo sapiens ; Mus musculus Manufacturer
Microarray Shared Resource, Comprehensive Cancer Center, The Ohio State University (OSU-CCC)
Manufacture protocol
Manufacured using GE Healthcare's CodeLink (TM) 3-D Gel Matrix Technology. CodeLink(tm) Activated Slides are designed to covalently immoblize amine-modified DNA for microarrays. The slides are coated with a 3-D surface chemistry comprised of a long-chain, hydrophilic polymer containing amine-reactive groups. This polymer is covalently crosslinked to itself and to the surface of the slide. The crosslinked polymer, combined with end-point attachment, orients the immobilized DNA, and holds it away from the surface of the slide. The 1x printing buffer consists of 50 mM sodium phosphate at pH 8.5. Codelink slides are manufactured by SurModics and were formally sold as 3D-Link(tm) Activated Slides. ¹ Microarrays are printed with an Omnigrid robotic arrayer by Genomic Solutions that is fitted with a printhead and 16 SMP3 print tips from Telechem. The microRNA microarray probe set is based on 40mer oligonucleotides from Sigma-Genosys. ¹ Oligonucleotide probes are distributed into 384-well polypropylene plates (Genetix, 384 micro array) and dissolved in print buffer (150mM sodium phosphate, pH 8.5, 4% sodium sulfate, 0.0001% N-lauryl sarcosyl) to a normalized 25 micromolar concentration. The miRNA microarray spot densities are 200 microns (center-to-center). The microarrays are printed on Codelink Activated Slide (PN300011)) and treated by a method that is an adaptation of the manufacturer's instructions to allow higher throughput processing.
Support
glass
Coating
polyacrymide hydrogel polymer
Submission date
Dec 26, 2006
Last update date
May 14, 2007
Contact name
Xin Wei Wang
E-mail(s)
xw3u@nih.gov Phone
240-760-6858
Organization name
National Cancer Institute
Department
Laboratory of Human Carcinogenesis
Lab
Liver Carcinogenesis Unit
Street address
37 Convent Drive
City
Bethesda
State/province
MD
ZIP/Postal code
20892-4255
Country
USA
Samples (709)
GSM157887 , GSM157888 , GSM157889 , GSM157890 , GSM157891 , GSM157892
GSM157893 ,
GSM157894 ,
GSM157895 ,
GSM157896 ,
GSM157897 ,
GSM157898 ,
GSM157899 ,
GSM157900 ,
GSM157901 ,
GSM157902 ,
GSM157903 ,
GSM157904 ,
GSM157905 ,
GSM157906 ,
GSM157907 ,
GSM157908 ,
GSM157909 ,
GSM157910 ,
GSM157911 ,
GSM157912 ,
GSM157913 ,
GSM157914 ,
GSM157915 ,
GSM157916 ,
GSM157917 ,
GSM157918 ,
GSM157919 ,
GSM157920 ,
GSM157921 ,
GSM157922 ,
GSM157923 ,
GSM157924 ,
GSM157925 ,
GSM157926 ,
GSM157927 ,
GSM157928 ,
GSM157929 ,
GSM157930 ,
GSM157931 ,
GSM157932 ,
GSM157933 ,
GSM157934 ,
GSM157935 ,
GSM157936 ,
GSM157937 ,
GSM157938 ,
GSM157939 ,
GSM157940 ,
GSM157941 ,
GSM157942 ,
GSM157943 ,
GSM157944 ,
GSM157945 ,
GSM157946 ,
GSM157947 ,
GSM157948 ,
GSM157949 ,
GSM157950 ,
GSM157951 ,
GSM157952 ,
GSM157953 ,
GSM157954 ,
GSM157955 ,
GSM157956 ,
GSM157957 ,
GSM157958 ,
GSM157959 ,
GSM157960 ,
GSM157961 ,
GSM157962 ,
GSM157963 ,
GSM157964 ,
GSM157965 ,
GSM157966 ,
GSM157967 ,
GSM157968 ,
GSM157969 ,
GSM157970 ,
GSM157971 ,
GSM157972 ,
GSM157973 ,
GSM157974 ,
GSM157975 ,
GSM157976 ,
GSM157977 ,
GSM157978 ,
GSM157979 ,
GSM157980 ,
GSM157981 ,
GSM157982 ,
GSM157983 ,
GSM157984 ,
GSM157985 ,
GSM157986 ,
GSM157987 ,
GSM157988 ,
GSM157989 ,
GSM157990 ,
GSM157991 ,
GSM157992 ,
GSM157993 ,
GSM157994 ,
GSM157995 ,
GSM157996 ,
GSM157997 ,
GSM157998 ,
GSM157999 ,
GSM158000 ,
GSM158001 ,
GSM158002 ,
GSM158003 ,
GSM158004 ,
GSM158005 ,
GSM158006 ,
GSM158007 ,
GSM158008 ,
GSM158009 ,
GSM158010 ,
GSM158011 ,
GSM158012 ,
GSM158013 ,
GSM158014 ,
GSM158015 ,
GSM158016 ,
GSM158017 ,
GSM158018 ,
GSM158019 ,
GSM158020 ,
GSM158021 ,
GSM158022 ,
GSM158023 ,
GSM158024 ,
GSM158025 ,
GSM158026 ,
GSM158027 ,
GSM158028 ,
GSM158029 ,
GSM158030 ,
GSM158031 ,
GSM158032 ,
GSM158033 ,
GSM158034 ,
GSM158035 ,
GSM158036 ,
GSM158037 ,
GSM158038 ,
GSM158039 ,
GSM158040 ,
GSM158041 ,
GSM158042 ,
GSM158043 ,
GSM158044 ,
GSM158045 ,
GSM158046 ,
GSM158047 ,
GSM158048 ,
GSM158049 ,
GSM158050 ,
GSM158051 ,
GSM158052 ,
GSM158053 ,
GSM158054 ,
GSM158055 ,
GSM158056 ,
GSM158057 ,
GSM158058 ,
GSM158059 ,
GSM158060 ,
GSM158061 ,
GSM158062 ,
GSM158063 ,
GSM158064 ,
GSM158065 ,
GSM158066 ,
GSM158067 ,
GSM158068 ,
GSM158069 ,
GSM158070 ,
GSM158071 ,
GSM158072 ,
GSM158073 ,
GSM158074 ,
GSM158075 ,
GSM158076 ,
GSM158077 ,
GSM158078 ,
GSM158079 ,
GSM158080 ,
GSM158081 ,
GSM158082 ,
GSM158083 ,
GSM158084 ,
GSM158085 ,
GSM158086 ,
GSM158087 ,
GSM158088 ,
GSM158089 ,
GSM158090 ,
GSM158091 ,
GSM158092 ,
GSM158093 ,
GSM158094 ,
GSM158095 ,
GSM158096 ,
GSM158097 ,
GSM158098 ,
GSM158099 ,
GSM158100 ,
GSM158101 ,
GSM158102 ,
GSM158103 ,
GSM158104 ,
GSM158105 ,
GSM158106 ,
GSM158107 ,
GSM158108 ,
GSM158109 ,
GSM158110 ,
GSM158111 ,
GSM158112 ,
GSM158113 ,
GSM158114 ,
GSM158115 ,
GSM158116 ,
GSM158117 ,
GSM158118 ,
GSM158119 ,
GSM158120 ,
GSM158121 ,
GSM158122 ,
GSM158123 ,
GSM158124 ,
GSM158125 ,
GSM158126 ,
GSM158127 ,
GSM170754 ,
GSM170755 ,
GSM170756 ,
GSM170757 ,
GSM170758 ,
GSM170759 ,
GSM170760 ,
GSM170761 ,
GSM170762 ,
GSM170763 ,
GSM170764 ,
GSM170765 ,
GSM170766 ,
GSM170767 ,
GSM170768 ,
GSM170769 ,
GSM170770 ,
GSM170771 ,
GSM170772 ,
GSM170773 ,
GSM170774 ,
GSM170775 ,
GSM170776 ,
GSM170777 ,
GSM170778 ,
GSM170779 ,
GSM170780 ,
GSM170781 ,
GSM170782 ,
GSM170783 ,
GSM170784 ,
GSM170785 ,
GSM170786 ,
GSM170787 ,
GSM170788 ,
GSM170789 ,
GSM170790 ,
GSM170791 ,
GSM170792 ,
GSM170793 ,
GSM170794 ,
GSM170795 ,
GSM170796 ,
GSM170797 ,
GSM170798 ,
GSM170799 ,
GSM170800 ,
GSM170801 ,
GSM170802 ,
GSM170803 ,
GSM170804 ,
GSM170805 ,
GSM170806 ,
GSM170807 ,
GSM170808 ,
GSM170809 ,
GSM170810 ,
GSM189827 ,
GSM189828 ,
GSM189829 ,
GSM189830 ,
GSM189831 ,
GSM189832 ,
GSM189833 ,
GSM189834 ,
GSM189835 ,
GSM189836 ,
GSM189837 ,
GSM189838 ,
GSM189839 ,
GSM189840 ,
GSM189841 ,
GSM189842 ,
GSM189843 ,
GSM189844 ,
GSM189845 ,
GSM189846 ,
GSM189847 ,
GSM189848 ,
GSM189849 ,
GSM189850 ,
GSM189851 ,
GSM189852 ,
GSM189853 ,
GSM189854 ,
GSM189855 ,
GSM189856 ,
GSM189857 ,
GSM189858 ,
GSM189859 ,
GSM189860 ,
GSM189861 ,
GSM189862 ,
GSM189863 ,
GSM189864 ,
GSM189865 ,
GSM189866 ,
GSM189867 ,
GSM189868 ,
GSM189869 ,
GSM189870 ,
GSM189871 ,
GSM189872 ,
GSM189873 ,
GSM189874 ,
GSM189875 ,
GSM189876 ,
GSM189877 ,
GSM189878 ,
GSM189879 ,
GSM189880 ,
GSM189881 ,
GSM189882 ,
GSM189883 ,
GSM189884 ,
GSM189885 ,
GSM189886 ,
GSM189887 ,
GSM189888 ,
GSM189889 ,
GSM189890 ,
GSM189891 ,
GSM189892 ,
GSM189893 ,
GSM189894 ,
GSM189895 ,
GSM189896 ,
GSM189897 ,
GSM189898 ,
GSM189899 ,
GSM189900 ,
GSM189901 ,
GSM189902 ,
GSM189903 ,
GSM189904 ,
GSM189905 ,
GSM189906 ,
GSM189907 ,
GSM189908 ,
GSM189909 ,
GSM189910 ,
GSM189911 ,
GSM189912 ,
GSM189913 ,
GSM189914 ,
GSM189915 ,
GSM189916 ,
GSM189917 ,
GSM189918 ,
GSM189919 ,
GSM189920 ,
GSM189921 ,
GSM189922 ,
GSM189923 ,
GSM189924 ,
GSM189925 ,
GSM189926 ,
GSM189927 ,
GSM189928 ,
GSM189929 ,
GSM189930 ,
GSM189931 ,
GSM189932 ,
GSM189933 ,
GSM189934 ,
GSM189935 ,
GSM189936 ,
GSM189937 ,
GSM189938 ,
GSM189939 ,
GSM189940 ,
GSM189941 ,
GSM189942 ,
GSM189943 ,
GSM189944 ,
GSM189945 ,
GSM189946 ,
GSM189947 ,
GSM189948 ,
GSM189949 ,
GSM189950 ,
GSM189951 ,
GSM189952 ,
GSM189953 ,
GSM189954 ,
GSM189955 ,
GSM189956 ,
GSM189957 ,
GSM189958 ,
GSM189959 ,
GSM189960 ,
GSM189961 ,
GSM189962 ,
GSM189963 ,
GSM189964 ,
GSM189965 ,
GSM189966 ,
GSM189967 ,
GSM189968 ,
GSM189969 ,
GSM189970 ,
GSM189971 ,
GSM189972 ,
GSM189973 ,
GSM189974 ,
GSM189975 ,
GSM189976 ,
GSM189977 ,
GSM189978 ,
GSM189979 ,
GSM189980 ,
GSM189981 ,
GSM189982 ,
GSM189983 ,
GSM189984 ,
GSM189985 ,
GSM189986 ,
GSM189987 ,
GSM189988 ,
GSM189989 ,
GSM189990 ,
GSM189991 ,
GSM189992 ,
GSM189993 ,
GSM189994 ,
GSM189995 ,
GSM189996 ,
GSM337903 ,
GSM337904 ,
GSM337905 ,
GSM337906 ,
GSM337907 ,
GSM337908 ,
GSM337909 ,
GSM337910 ,
GSM337911 ,
GSM337912 ,
GSM337913 ,
GSM337914 ,
GSM337915 ,
GSM337916 ,
GSM337917 ,
GSM337918 ,
GSM337919 ,
GSM337920 ,
GSM337921 ,
GSM337922 ,
GSM337923 ,
GSM337924 ,
GSM337925 ,
GSM337926 ,
GSM337927 ,
GSM337928 ,
GSM337929 ,
GSM337930 ,
GSM337931 ,
GSM337932 ,
GSM337933 ,
GSM337934 ...
Accession list truncated, click here to browse through all related public accessions You can also download a list of all accessions here Series (3)
GSE6857 Unique MicroRNA Expression Signature Predicts Survival, Metastases and Recurrence of HCC
GSE7055 Expression of microRNAs and Protein-coding Genes Associated with Perineural Invasion in Prostate Cancer
GSE7828 MicroRNA profiles of 84 colon adenocarcinomas and paired nontumorous
Data table header descriptions ID unique spot id
Slide_block Array block location
Slide_column Array column location
Slide_row Array row location
OLIGO_ID Oligo identifier
DESCRIPTION Description of content
MATURE_SANGER_REGISTRY_NAME Registry name in Sanger's mirBASE, http://microrna.sanger.ac.uk
ORF ORGANISM SPOT_ID
Data table ID Slide_block Slide_column Slide_row OLIGO_ID DESCRIPTION MATURE_SANGER_REGISTRY_NAME ORF ORGANISM SPOT_ID 1-1-1
1
1
1
hsa-mir-033b-prec
hsa-mir-033b-prec
hsa-mir-033b
mirn033b
Homo sapiens
1-1-2
1
1
2
hsa-mir-125a-precNo2
hsa-mir-125a-precNo2
hsa-mir-125a
mirn125a
Homo sapiens
1-1-3
1
1
3
hsa-mir-153-1-prec1
hsa-mir-153-1-prec1
hsa-mir-153-1
mirn153-1
Homo sapiens
1-1-4
1
1
4
uc.124+
Control
Control
1-1-5
1
1
5
uc.156+
Control
Control
1-1-6
1
1
6
uc.6+A
Control
Control
1-1-7
1
1
7
uc.52+A
Control
Control
1-1-8
1
1
8
uc.84+A
Control
Control
1-1-9
1
1
9
uc.320+A
Control
Control
1-1-10
1
1
10
uc.364+A
Control
Control
1-1-11
1
1
11
mmu-mir-138-1No2
mmu-mir-138-1No2
mmu-mir-138-1
mirn138-1
Mus musculus
1-1-12
1
1
12
mmu-mir-96No2
mmu-mir-96No2
mmu-mir-96
mirn96
Mus musculus
1-1-13
1
1
13
hsa-mir-101-2No1
hsa-mir-101-2No1
hsa-mir-101-2
mirn101-2
Homo sapiens
1-1-14
1
1
14
hsa-mir-371No1
hsa-mir-371No1
hsa-mir-371
mirn371
Homo sapiens
1-1-15
1
1
15
Blank
Blank
Blank
1-1-16
1
1
16
Blank
Blank
Blank
1-1-17
1
1
17
Blank
Blank
Blank
1-1-18
1
1
18
Blank
Blank
Blank
1-2-1
1
2
1
hsa-mir-033b-prec
hsa-mir-033b-prec
hsa-mir-033b
mirn033b
Homo sapiens
1-2-2
1
2
2
hsa-mir-125a-precNo2
hsa-mir-125a-precNo2
hsa-mir-125a
mirn125a
Homo sapiens
11520 610 Kbytes .Supplementary data files not provided
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