Printing by NIAID / Microarray Research Facility Surface type: non-adsorptive, polylysine-coated glass Attachment: covalent, (UV-treated polylysine) Array description: The custom microarrays are manufactured by the Microarray Research Facility of the National Institute of Allergy and Infectious Diseases (NIH/NIAID). Microarrays are printed with an Omnigrid robotic arrayer by Genomic Solutions (http://www.genomicsolutions.com ) that is fitted with a printhead and 32 SMP3 print tips from Telechem (http://www.arrayit.com ). The mouse microarray (Mmaa) probe set is based on 70mer oligonucleotides from Qiagen's Mouse Genome Oligo Set V1.0 (http://oligos.qiagen.com/arrays/omad.php ) -- version 1.0 contains oligo IDs M000001_01 through M006868_01 of the version 1.1 set listed at this URL. Oligonucleotide probes are distributed into 384-well polypropylene plates (Genetix, 384 micro array) and dissolved in 4XSSC print buffer at a normalized 25 micromolar concentration. The Mmaa microarray spot densities are 190 microns (center-to-center). Each probe is printed in duplicate as side-by-side features. The microarrays are printed on Fisher Premium microscope slides (cat# 12-544-1) coated with poly-L-lysine and post-processed according to the procedures on the Stanford website: http://cmgm.stanford.edu/pbrown/protocols/index.html .
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