GEO help: Mouse over screen elements for information.
Status
Public on Mar 14, 2007
Title
mwg-10k-2
Technology type
spotted oligonucleotide
Distribution
non-commercial
Organism
Mus musculus Manufacturer
Gentron
Manufacture protocol
50 mer mouse 10K oligo-set (MWG, Germany) were resuspended in 15 ul of DMSO 50% (Stock solution plates). 5 ul from each oligo of the stock solution were transferred to a 384 well plate (Whatman Inc) (Working solution plates). Oligos in the working solution plates were printed onto UltraGap Slides (Corning) using a Versarray chipwriter (Bio-Rad) with Stealth Micro Spotting quill pins (Telechem). Humidity was set at 45%, and temperature was 22-25°C. Pins were loaded in the working plate during 0.5 sec. Distance between spots was 292 um, Dwell time for printing was 0.05 sec, each oligo was spotted one time per slide. After the pins were loaded in the working plate and before printing the slides, a blotting slide was printed 5 times in order to discard the excess of oligo on the pins. After printing on slides, and before loading a new oligo, pins were washed with 3 cycles of 5 sec. wash with water, followed by 1 sec vacuum, the last vacuum time was 10 sec. After printing was completed, oligos were UV crosslinked to the slides on a CL-1000 UV crosslinker (UVP). Printed slides were stored in a dissecator
Support
glass
Coating
aminosilane
Contributor(s)
Salibe MC , Alvarez MJ , Serra CG , Celi DC , Vila-Ortiz G , Ramirez A , Podhajcer OL , Pitossi FJ Submission date
Mar 09, 2007
Last update date
Mar 14, 2007
Contact name
Mariano Cesar Salibe
E-mail(s)
msalibe@leloir.org.ar Organization name
Gentron LLC
Street address
Av. Patricias Argentinas 435
City
Ciudad de Buenos Aires
ZIP/Postal code
1425
Country
Argentina
Samples (25)
GSM174918 , GSM174919 , GSM174920 , GSM174921 , GSM175060 , GSM175061
GSM175082 ,
GSM175109 ,
GSM175140 ,
GSM175141 ,
GSM175142 ,
GSM175143 ,
GSM175225 ,
GSM175226 ,
GSM175227 ,
GSM175228 ,
GSM175306 ,
GSM175376 ,
GSM175377 ,
GSM175378 ,
GSM175379 ,
GSM175409 ,
GSM175410 ,
GSM175411 ,
GSM175412 Series (10)
GSE7287 profiling of prefrontal cortex from mice s.c. injected with CT-26wt colon carcinoma cells vs. PBS
GSE7293 profiling of prefrontal cortex from mice s.c. injected with 4T-1 mammary carcinoma cells vs. PBS
GSE7294 profiling of hypothalamus from mice s.c. injected with CT-26wt colon carcinoma cells vs. PBS
GSE7295 profiling of hypothalamus from mice s.c. injected with LL/2(LLC1) lung carcinoma cells vs. PBS
GSE7296 profiling of hypothalamus from mice s.c. injected with 4T-1 mammary carcinoma cells vs. PBS
GSE7297 profiling of midbrain from mice s.c. injected with CT-26wt colon carcinoma cells vs. PBS
GSE7298 profiling of midbrain from mice s.c. injected with 4T-1 mammary carcinoma cells vs. PBS
GSE7299 profiling of midbrain from mice s.c. injected with LL/2(LLC1) lung carcinoma cells vs. PBS
GSE7300 profiling of prefrontal cortex from mice s.c. injected with LL/2(LLC1) lung carcinoma cells vs. PBS
GSE7565 Profiling brain and liver tissues from mice
Data table header descriptions ID GB_ACC GenBank accession number of sequence used to design oligonucleotide probe
GENE_NAME Descriptive gene name
SPOT_IDENTIFIER Alternative spot identifier
TIP_ID Print tip ID
M_ROW Grid row
M_COLUMN Grid column
SPOT_ID spot identifier
Data table ID GB_ACC GENE_NAME SPOT_IDENTIFIER TIP_ID M_ROW M_COLUMN SPOT_ID 1
NM_019446 barh-like drosophila barhl1
mwgmouse10K00442
1
1
1
2
AK016573 adult male testis riken cdna clone:4932443l08
mwgmouse10K00375
1
1
1
3
AF109905 major histocompatibility locus class iii regions hsc70t smrnp g7a ng23 muts homolog clcp ng24 ng25 and ng26s unknown
mwgmouse10K00302
1
1
1
4
NM_020267 brain cdna mc7-pending; putative mc7 protein
mwgmouse10K00229
1
1
1
5
NM_008084 Replicate adult male kidney riken cdna clone:0610007c02; glyceraldehyde-3-phosphate dehydrogenase
mwgmouse10K06197-r1
1
1
1
6
NM_054096 clone mgc:7116
mwgmouse10K00085
1
1
1
7
NM_011977 solute carrier family 27 fatty acid transporter member slc27a1
mwgmouse10K00446
1
1
1
8
AK007868 10 day old male pancreas riken cdna clone:1810055g02
mwgmouse10K00379
1
1
1
9
AK009960 adult male tongue riken cdna clone:2310057j16
mwgmouse10K00308
1
1
1
10
NM_030703 carboxypeptidase n small subunit
mwgmouse10K00235
1
1
1
11
AK005678 13 days embryo liver riken cdna clone:2510044o04
mwgmouse10K00162
1
1
1
12
NM_007642 glycoprotein cd28 precursor; 3 end
mwgmouse10K00089
1
1
1
13
NM_008672 nucleosome assembly protein 1-like 4 nap1l4
mwgmouse10K00451
1
1
1
14
BC004710 adult male tongue riken cdna clone:2310045l10; 2310045l10 clone mgc:7806
mwgmouse10K00385
1
1
1
15
NM_030239 atp-binding cassette sub-family g white member abcg3
mwgmouse10K00312
1
1
1
16
NM_008747 brain neurotensin receptor g-protein coupled
mwgmouse10K00240
1
1
1
17
NM_011066 period 2 per2 kiaa0347 encoded by genbank accession number ab002345 and the d. melanogaster circadian clock pe; homolog drosophila
mwgmouse10K00166
1
1
1
18
NM_007498 transcription factor lrg-21 leucine zipper protein; activating 3 atf3
mwgmouse10K00094
1
1
1
19
AK006318 adult male testis riken cdna clone:1700025a19
mwgmouse10K00456
1
1
1
20
--Arabidopsis Control Oligo
2-ara7
1
1
1
--Arabidopsis Control Oligo
10080 868 Kbytes .Supplementary data files not provided
Less...
More...