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Status
Public on Mar 14, 2007
Title
mwg-10k-3
Technology type
spotted oligonucleotide
Distribution
non-commercial
Organism
Mus musculus Manufacturer
Gentron
Manufacture protocol
50 mer mouse 10K oligo-set (MWG, Germany) were resuspended in 15 ul of DMSO 50% (Stock solution plates). 5 ul from each oligo of the stock solution were transferred to a 384 well plate (Whatman Inc) (Working solution plates). Oligos in the working solution plates were printed onto UltraGap Slides (Corning) using a Versarray chipwriter (Bio-Rad) with Stealth Micro Spotting quill pins (Telechem). Humidity was set at 45%, and temperature was 22-25°C. Pins were loaded in the working plate during 0.5 sec. Distance between spots was 292 um, Dwell time for printing was 0.05 sec, each oligo was spotted one time per slide. After the pins were loaded in the working plate and before printing the slides, a blotting slide was printed 5 times in order to discard the excess of oligo on the pins. After printing on slides, and before loading a new oligo, pins were washed with 3 cycles of 5 sec. wash with water, followed by 1 sec vacuum, the last vacuum time was 10 sec. After printing was completed, oligos were UV crosslinked to the slides on a CL-1000 UV crosslinker (UVP). Printed slides were stored in a dissecator
Support
glass
Coating
aminosilane
Contributor(s)
Salibe MC , Alvarez MJ , Serra CG , Celi DC , Vila-Ortiz G , Ramirez A , Podhajcer OL , Pitossi FJ Submission date
Mar 09, 2007
Last update date
Mar 14, 2007
Contact name
Mariano Cesar Salibe
E-mail(s)
msalibe@leloir.org.ar Organization name
Gentron LLC
Street address
Av. Patricias Argentinas 435
City
Ciudad de Buenos Aires
ZIP/Postal code
1425
Country
Argentina
Samples (16)
GSM175083 , GSM175084 , GSM175085 , GSM175110 , GSM175111 , GSM175112
Series (6)
GSE7287 profiling of prefrontal cortex from mice s.c. injected with CT-26wt colon carcinoma cells vs. PBS
GSE7294 profiling of hypothalamus from mice s.c. injected with CT-26wt colon carcinoma cells vs. PBS
GSE7295 profiling of hypothalamus from mice s.c. injected with LL/2(LLC1) lung carcinoma cells vs. PBS
GSE7296 profiling of hypothalamus from mice s.c. injected with 4T-1 mammary carcinoma cells vs. PBS
GSE7297 profiling of midbrain from mice s.c. injected with CT-26wt colon carcinoma cells vs. PBS
GSE7565 Profiling brain and liver tissues from mice
Data table header descriptions ID GB_ACC GenBank accession number of sequence used to design oligonucleotide probe
GENE_NAME Descriptive gene name
SPOT_IDENTIFIER Alternative spot identifier
TIP_ID Print tip ID
M_ROW Grid row
M_COLUMN Grid column
SPOT_ID spot identifier
Data table ID GB_ACC GENE_NAME SPOT_IDENTIFIER TIP_ID M_ROW M_COLUMN SPOT_ID 1
NM_019446 barh-like drosophila barhl1
mwgmouse10K00442
1
1
1
2
NM_013751 hras suppressor expressed in skeletal muscle heart brain and bone marrow; 18 days embryo riken cdna clone:1190010i23
mwgmouse10K00341
1
1
1
3
NM_020267 brain cdna mc7-pending; putative mc7 protein
mwgmouse10K00229
1
1
1
4
NM_020483 11 days embryo riken cdna clone:2700016d05 full insert sequence; hcngp
mwgmouse10K00121
1
1
1
5
NM_011977 solute carrier family 27 fatty acid transporter member slc27a1
mwgmouse10K00446
1
1
1
6
AK015698 adult male testis riken cdna clone:4930505a04
mwgmouse10K00345
1
1
1
7
NM_030703 carboxypeptidase n small subunit
mwgmouse10K00235
1
1
1
8
NM_015797 adult male kidney riken cdna clone:0610039i14; f-box protein fbx6b
mwgmouse10K00127
1
1
1
9
NM_008672 nucleosome assembly protein 1-like 4 nap1l4
mwgmouse10K00451
1
1
1
10
AK008024 adult male small intestine riken cdna clone:2010001p20
mwgmouse10K00349
1
1
1
11
NM_008747 brain neurotensin receptor g-protein coupled
mwgmouse10K00240
1
1
1
12
NM_024187 adult male small intestine riken cdna clone:2010107d16; 10 11 days embryo clone:2810032c18
mwgmouse10K00133
1
1
1
13
AK006318 adult male testis riken cdna clone:1700025a19
mwgmouse10K00456
1
1
1
14
NM_025505 adult male testis riken cdna clone:1700030g05; 1700030g05 1700030g05rik
mwgmouse10K00354
1
1
1
15
NM_010221 fk506 binding protein 6 65 kda fkbp6
mwgmouse10K00244
1
1
1
16
AK013374 10 11 days embryo riken cdna clone:2810462m08
mwgmouse10K00137
1
1
1
17
AK009097 adult male tongue riken cdna clone:2310002l09
mwgmouse10K00868
1
1
1
18
NM_020588 hypothetical protein mncb-2755 clone mgc:7071; adult male liver riken cdna clone:1300007b12
mwgmouse10K00764
1
1
1
19
NM_009523 wingless-related mmtv integration site 4 wnt4
mwgmouse10K00660
1
1
1
20
AK005108 adult male cerebellum riken cdna clone:1500002c15
mwgmouse10K00554
1
1
1
10080 870 Kbytes .Supplementary data files not provided
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